Method for preparing lactalbumin hydrolysate without chloropropanol by utilizing soybean meal
A technology of chloropropanol protein and soybean protein, which is applied in the field of protein hydrolyzate preparation, can solve the problems of affecting the taste and high cost of enzymatic hydrolysis, and achieve the effects of prolonging the shelf life, improving the overall flavor, and shortening the production cycle
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[0030] Example one
[0031] The method for preparing non-chloropropanol protein hydrolysate using soybean meal, the steps are as follows:
[0032] (1) Raw material pretreatment
[0033] Weigh 150kg of defatted soybean meal and crush it to 60 mesh;
[0034] (2) Hydrolysis of soy protein
[0035] 2.1 Put the defatted soybean meal into a 2000 liter reaction tank, add 850 liters of pure water, and stir at a speed of 100 rpm; steam the interlayer and raise it to 60°C;
[0036] 2.2 Adjust pH 5.0;
[0037] 2.3 Add 0.075kg papain, stir and react at 60-62°C for 12 hours at a stirring speed of 100rpm;
[0038] 2.4 Add 0.075kg of Novozymes flavor protease, stir and react at 60-62°C for 12 hours at a stirring speed of 100rpm;
[0039] (3) Fermentation of proteolytic solution
[0040] 3.1 Cool the proteolysis solution to 30°C;
[0041] 3.2 Add 0.15kg of lactic acid bacteria powder and 0.15kg of yeast powder, and ferment at 30-32℃ for 24 hours;
[0042] 3.3 Raise the temperature to 95°C for 10 minutes to st...
Example Embodiment
[0053] Example two
[0054] The method for preparing non-chloropropanol protein hydrolysate using soybean meal, the steps are as follows:
[0055] (1) Raw material pretreatment
[0056] Weigh 150kg of defatted soybean meal and crush it to 70 mesh;
[0057] (2) Hydrolysis of soy protein
[0058] 2.1 Put the defatted soybean meal into a 2000 liter reaction tank, add 850 liters of pure water, and stir at a stirring speed of 100 rpm; steam the interlayer and heat it up to 40°C;
[0059] 2.2 Adjust pH 7.0;
[0060] 2.3 Add 3kg of neutral protease, stir and react at 60 to 62°C for 1 hour at a stirring speed of 100rpm;
[0061] 2.4 Add 3kg of Danisco protein exonuclease, stir and react at 60-62°C for 1 hour at a stirring speed of 100rpm;
[0062] (3) Fermentation of proteolytic solution
[0063] 3.1 Cool the proteolysis solution to 30°C;
[0064] 3.2 Add 2kg of lactic acid bacteria powder and 2kg of yeast powder, and ferment at 30-32℃ for 6 hours;
[0065] 3.3 Raise the temperature to 95°C for 10 min...
Example Embodiment
[0073] Example three
[0074] The method for preparing non-chloropropanol protein hydrolysate using soybean meal, the steps are as follows:
[0075] (1) Raw material pretreatment
[0076] Weigh 150kg of defatted soybean meal and crush it to 80 mesh;
[0077] (2) Hydrolysis of soy protein
[0078] 2.1 Put the defatted soybean meal into a 2000 liter reaction tank, add 850 liters of pure water, and stir at a speed of 100 rpm; steam the interlayer and raise it to 60°C;
[0079] 2.2 Adjust pH to 8.0;
[0080] 2.3 Add 1.5kg of alkaline protease papain, stir and react at 60 to 62°C for 6 hours at a stirring speed of 100rpm;
[0081] 2.4 Add 1.5kg of Novozymes flavor protease, stir and react at 60-62°C for 6 hours at a stirring speed of 100rpm;
[0082] (3) Fermentation of proteolytic solution
[0083] 3.1 Cool the proteolysis solution to 30°C;
[0084] 3.2 Add 1kg of lactic acid bacteria powder and 1kg of yeast powder, and ferment at 30-32℃ for 12 hours;
[0085] 3.3 Raise the temperature to 95°C for...
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