Method for extracting selaginella biflavone

A technology of biflavonoids and biflavones of Selaginella, applied in the field of extracting biflavones of Selaginella, can solve the problems of reducing the adsorption effect of resin columns, achieve the effects of low cost, simple extraction process, and reduced energy consumption

Inactive Publication Date: 2011-09-14
NANJING ZELANG AGRI DEV
View PDF2 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not go through concentration, and is directly applied to the polyamide column f

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Weigh 200g of 80-mesh Selaginella powder, add 1600ml of petroleum ether to carry out Soxhlet extraction, the extraction temperature is 60-90°C, the extraction time is 1h, the residual petroleum ether is volatilized from the raffinate, and the petroleum ether raffinate is obtained. The remainder was extracted with 10 times the amount of acetone under heating and reflux, and repeated twice. The extracts were combined and concentrated by rotary evaporation to obtain the acetone concentrate; the pH of the acetone concentrate was adjusted to 3.5, and polyethylene glycol 6000 and ammonium sulfate were added in sequence to make the formed The mass concentration of polyethylene glycol in the aqueous two-phase extraction system is 15%, the mass concentration of ammonium sulfate is 12%, stirring and extracting at 25°C for half an hour, and then standing for 10 hours to form two phases, Selaginella biflavone enters For the polyethylene glycol upper phase, use an ultrafiltration mem...

Embodiment 2

[0013] Weigh 200g of 40-mesh Selaginella powder, add 1400ml of petroleum ether to carry out Soxhlet extraction, the extraction temperature is 60-90°C, the extraction time is 2h, the residual petroleum ether is volatilized from the raffinate, and the petroleum ether raffinate is obtained. The remainder was extracted with 8 times the amount of acetone under heating and reflux, repeated 3 times, the extracts were combined, concentrated by rotary evaporation to obtain the acetone concentrate; the pH of the acetone concentrate was adjusted to 4.0, and polyethylene glycol 4000 and sodium sulfate were added in sequence to make the formed The mass concentration of polyethylene glycol in the aqueous two-phase extraction system is 30%, the mass concentration of sodium sulfate is 13%, stirring and extracting at a temperature of 30°C for half an hour, and then standing for 13 hours to form two phases, Selaginella biflavone enters For the polyethylene glycol upper phase, use an ultrafiltrat...

Embodiment 3

[0015] Weigh 200g of 40-mesh Selaginella powder, add 2000ml of petroleum ether to carry out Soxhlet extraction, the extraction temperature is 60-90°C, the extraction time is 2h, the residual petroleum ether is volatilized from the raffinate, and the petroleum ether raffinate is obtained. The remainder was extracted with 12 times the amount of acetone under reflux, repeated twice, the extracts were combined, concentrated by rotary evaporation to obtain the acetone concentrate; the pH of the acetone concentrate was adjusted to 3.0, and polyethylene glycol 6000 and potassium carbonate were added in sequence to make the formed The mass concentration of polyethylene glycol in the aqueous two-phase extraction system is 25%, the mass concentration of potassium carbonate is 15%, stirring and extracting at 28°C for half an hour, and then standing for 12 hours to form two phases, Selaginella biflavone enters For the polyethylene glycol upper phase, use an ultrafiltration membrane with a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting selaginella biflavone, and belongs to the technical field of chemical extraction process of medicinal plants. The method comprises the following steps of: extracting selaginella serving as a raw material by using petroleum ether and acetone sequentially, adding acetone extracting solution into inorganic salt solution and polyethylene glycol, stirring, mixing fully for extracting, and standing to form two phases; and allowing selaginella biflavone to enter a polyethylene glycol top phase, separating the selaginella biflavone from polyethylene glycol by top phase liquid through an ultrafiltration membrane method, concentrating the selaginella biflavone by using a nanofiltration membrane, reconcentrating and drying to obtain a selaginella biflavone product. In the method, the technology of two aqueous phase extraction and membrane concentration is adopted, so the method adopts a simple extraction process and has low requirement on equipment, and is suitable for industrial production.

Description

Technical field: [0001] The invention relates to a method for extracting selaginella biflavones. Background technique: [0002] Selaginella is widely distributed, and there are more than 700 species in the world, and there are more than 70 species in my country. Modern pharmacological studies have shown that Selaginella not only has the traditional anti-inflammatory and detoxifying effects, but also has the effects of lowering blood pressure, lowering blood sugar, antibacterial, antiviral, enhancing immune function, and inhibiting oxidative stress. The chemical components of Selaginella are flavonoids, alkaloids, phenols, organic acids, amino acids and polysaccharides, etc., and the content of biflavonoids, mainly biflavonoids, is the highest. The method disclosed in Chinese Patent Application No. 200810197033.4 is to dry Selaginella chinensis plant material, remove impurities, pulverize, reflux degrease with petroleum ether, then reflux extract with 40%-95% ethanol solutio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/11A61K31/352
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap