Construction method of genome-wide mRNA 3' end gene library

A technology of gene library and whole genome, applied in the field of preparation of whole genome mRNA 3' end library, can solve the problems affecting the sequencing process and results, and achieve the effect of increasing anchoring efficiency, simple sample preparation steps, and ensuring integrity.
CN102181527BInactive Publication Date: 2014-10-22SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2014-10-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of a terminal gene library of full genome mRNA3' and is suitable for high-throughput sequencing. A cDNA-chain is synthesized by transforming a template, and mutation is induced into polymerase chain reaction (PCR) to obtain a non-translated regional deoxyribonucleic acid (DNA) sequence of the full genome mRNA3'. The data yield of the library prepared by the preparation method of the terminal library of the full genome mRNA3' is large; the sample preparation steps are simple and economic; and the preparation method is beneficial for analysis of later-stage bioinformatics, and is easy for large-scale applications.
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Description

technical field

[0001] The invention relates to a method for constructing a gene library, in particular to a method for preparing a whole-genome mRNA 3' end library suitable for high-throughput sequencing. Background technique

[0002] The 3' untranslated region (3'UTR for short) is a special part of messenger RNA (mRNA) located at the 3' end of the coding region of a gene. 3'UTR is an important regulatory element of mRNA function, and has the following regulatory sequences: polyadenylation site, usually AAUAAA; protein binding site and miRNA target site, etc. 3'UTR can not only control the in vivo stability and degradation rate of mRNA, regulate the subcellular localization and translation level of mRNA, but also determine the type of cell it is expressed in, control the utilization efficiency of mRNA, and assist in the identification of special codons. Mutations in the 3'UTR can affect the expression of one or more genes, leading to disease. Human genes often have multip...

Claims

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