Method for biological detoxication of pretreated lignocellulose biomass

A lignocellulose and pretreatment technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems affecting the continuity of the simultaneous saccharification fermentation process and the long detoxification period.

Inactive Publication Date: 2011-09-21
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially the detoxification effect of fungi in the hydrolyzed solution after pretreatment is the most obvious. Lopez equals to 2004 and screened out Coniochaeta ligniaria, which can completely degrade furfural and hydroxymethylfurfural (concentrations are respectively 20mM and 15mM) in the hydrolyzed solution. The disadvantage is that the detoxification period is long
Regardless of the detoxification method, most of the detoxification methods currently used are to detoxify the hydrolyzate after enzymatic hydrolysis, which seriously affects the continuity of the simultaneous saccharification and fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]The corn stalks obtained by steam expansion pretreatment were detoxified by A.resinae ZN1, and then the low-solid content shake-flask synchronous saccharification and fermentation: A.resinae ZN1 spores were eluted from the slant of the test tube with sterile water. The spore suspension was added to the steam-expanded pretreated corn stover (10% inoculum size, 60% water content). Place them in a 25°C incubator and cultivate them for 3 days as seeds. The steam-expanded corn stalks with a water content of 60% were put into a 250ml shaker flask, and the seed stalks were inserted and treated continuously for 5 days (25° C.). Add citric acid buffer solution with pH 4.8 and an appropriate amount of enzyme solution to the shake flask, so that the solid content is 10%, and the enzyme activity is 15 FPU / gDM. After 12 hours of saccharification (50°C, 200rpm), sample HPLC was used to detect the concentration of inhibitors, and the fermentation was continued with acclimated yeast fo...

Embodiment 2

[0034] The corn stalks obtained by the steam expansion pretreatment were detoxified by A. resinae ZN1, and the high solid content was saccharified and fermented in a 5L reactor simultaneously: the seed corn stalks were cultivated in the same way as in Example 1. Use deionized water to adjust the fresh ones, and treat the obtained corn stalks in the same way to make the water content 60%. The seeds were inserted into corn stalks and treated continuously for 4 days (25°C). The detoxified corn stalks, YPD nutrient salt solution, and enzyme solution were placed in a 5L reactor, with a final solid content of 30% and an enzyme activity of 15FPU / gDM. And set the temperature at 50° C., rotate at 150 rpm, saccharify for 12 hours, and take samples to detect the concentration of inhibitors therein. Immediately insert acclimatized yeast seeds (inoculum size 10%), ferment for 60 hours at 37° C. and 150 rpm, and regularly take samples to detect ethanol production. Under the same condition...

Embodiment 3

[0036] Corn stover pretreated with dilute acid was detoxified by A.resinae ZN1 and low solids content shake-flask simultaneous saccharification and fermentation: pH of corn stover treated with dilute acid was adjusted with sodium hydroxide solution. Cultivate seed corn stalks in the same manner as in Example 1. Lignocellulosic raw materials were pretreated with dilute acid with a water content of 60%, put into a 250ml shake flask, inserted with seed stalks, and treated continuously for 4 days (25° C.). Add citrate buffer solution with a pH of 4.8 and an appropriate amount of enzyme solution to the shake flask, so that the solid content is 10%, and the enzyme activity is 15 FPU / gDM. After saccharification for 12h (50°C, 200rpm), samples were taken to detect the concentration of inhibitors by HPLC, and then acclimatized yeast were added for fermentation (37°C, 150rmp, 24h) to be sampled to detect the concentration of ethanol. Under the same conditions, the concentrations of var...

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Abstract

The invention relates to a method for biological detoxication of pretreated lignocellulose biomass, which comprises the following steps: step one, performing pretreatment for preparation of raw materials; and step two, performing biological detoxication and fermentation: inoculating fungi Amorphotheca resinae ZN1 by using PDA as a medium, followed by cultivation at a temperature of 20 to 30 DEG C; performing elution to obtain A. resinae ZN1 spores, inserting the A. resinae ZN1 spores into the pretreated lignocellulose biomass raw materials, and carrying out cultivation until the growing of mycelia, so that a first order seed is obtained; and inserting the first order seed into the raw materials, wherein the water content is 10 to 90%, adjusting the pH of the raw materials to 4 to 7, and then carrying out cultivation for 1 to 7 days at a temperature of 20 to 40 DEG C. The method for biological detoxication of the pretreated lignocellulose biomass provided in the invention has advantages of simple operation and low requirement for equipment. Furthermore, various mainly inhibitors produced during the pretreatment processing can be degraded. Therefore, the continuity of simultaneous saccharification and fermentation of high-solid content can be ensured, processing steps can be reduced, and working procedure time can be shorten and working procedure cost can be reduced.

Description

【Technical field】 [0001] The invention relates to a method for rapid biological detoxification of degradation products produced by lignocellulosic biomass pretreated by physical, chemical or physicochemical methods in the form of solid-state culture of fungi. After the pretreated lignocellulosic biomass is biologically detoxified, even under the condition of high solid content (high inhibitor concentration will be caused without detoxification), it can also normally carry out synchronous saccharification and fermentation operations to produce ethanol, Specifically, it is a method for biological detoxification of lignocellulosic biomass after pretreatment. 【Background technique】 [0002] The production of fuel ethanol from lignocellulosic biomass can not only solve the energy crisis caused by the depletion of fossil fuels, but also alleviate the greenhouse effect caused by fossil fuels. More importantly, most lignocellulosic biomass is agricultural and forestry waste, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/645
CPCY02E50/16Y02E50/10
Inventor 鲍杰朱智楠张建
Owner EAST CHINA UNIV OF SCI & TECH
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