Calicivirus inactivation method
A calicivirus inactivation technology, applied in the direction of antiviral agents, chemical instruments and methods, botanical equipment and methods, etc., can solve problems such as rough skin, unknown effect of norovirus, strong odor, etc., and achieve the purpose of inhibiting the spread Effect
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example 1
[0068] Example 1 (reference example) to feline calicivirus infectious inactivation effect test (alcohol concentration dependence)
[0069] Use M / 100 phosphate buffer solution pH7.2 (hereinafter referred to as PBS) to dilute the 99.5% special grade ethanol reagent into 80%, 70%, 60%, 50%, 40%, 30%, 20% in W / W% %, thereby preparing a sample, and measuring the disinfection effect (virus inactivation effect) of the sample by the test method shown below. The results are shown in Table 1.
example 2
[0086] Example 2 (reference example) to the infectious inactivation effect test of feline calicivirus (pH dependence containing ethanol solution) 1
[0087] Prepare samples with the composition shown in Table 2, and measure the disinfection effect (virus inactivation effect) of the sample according to the test method described in Example 1. The results are shown in Table 3.
[0088] Table 2
[0089] Composition of samples 1 and 2 (%W / W)
[0090]
sample 1
sample 2
ethanol
50.0
68.0
Glycerin Fatty Acid Ester※
2.0
5.0
-
10.0
2.0
-
water
46.0
17.0
[0091] ※Glycerin fatty acid ester: glycerin (mono)caprate
[0092] table 3
[0093] Test result 2
[0094]
[0095] Summary: It can be seen that the disinfection effect on feline calicivirus was not confirmed in the sample 1 showing alkalinity; the disinfection effect was confirmed in the sample 2 ...
example 3
[0096] Example 3 (reference example) to the infectious inactivation effect test of feline calicivirus (pH dependence containing ethanol solution) 2
[0097] Add an equal amount of feline calicivirus stock solution to the sample described in Table 4, and after reacting at room temperature for 1 minute, dilute to 10 times with 5-fold concentration of broth (80 mg / ml of protein) to make the cells Toxicity disappears. Then after performing 10-fold serial dilution and diluting to 10 -6 For the dilution series of the samples, use MEM as the diluent. Inoculate 100 μl of each dilution in 3 wells of a 6-well plate in confluent cat liver cells (CrFK) that have been cultured for 3 days. After 90 minutes of virus adsorption, cover with MEM medium containing 0.7% agar . At 34°C, 5% CO 2 cultured in an incubator for 50 hours. After fixing with 10% formalin for several hours, remove the agar medium, add methylene blue staining solution, and count the number of stained plaques. The resu...
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