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Method for separating CGMP (casein glycomacropeptide) having high content of sialic acid by using ion exchange resin

A casein glycomacropeptide and ion exchange resin technology, applied in the field of active substance separation, can solve the problems of failure to achieve industrialization, low sialic acid content, and high production costs, and achieve improved comprehensive utilization, low prices, and reduced sample costs. The effect of loss

Inactive Publication Date: 2011-10-19
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods for purifying CGMP, such as precipitation method, ultrafiltration method, two-phase system method, enzyme cross-linking method, etc., but these methods have disadvantages such as low sialic acid content and high production cost. Therefore, at present, CGMP, especially high sialic acid Content CGMP production has not yet achieved industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of liquid raw materials containing casein glycomacropeptide

[0031] Take D90 desalted whey powder (protein content 14%, mass fraction; sialic acid content 0.74g / 100g whey powder) and dissolve in 0.02mol / L sodium acetate solution to make the final concentration 100mg / ml. Centrifuge at 500×g for 20 minutes to remove insoluble matter. The supernatant after centrifugation was adjusted to pH 5.0 with glacial acetic acid for later use.

[0032] (2) Treatment of D061 ion exchange resin

[0033] The newly-purchased resin can be used only after it has been activated. After it is fully swollen, it is alternately rinsed with an acid-base solution for transformation and activation. Finally, it is treated with dilute hydrochloric acid to become a hydrogen-form resin. The specific method is as follows.

[0034] Treatment of cation exchange resin: take D061 cation exchange resin, place it in deionized water to swell for 24 hours, soak it in 2% hydrochloric acid wit...

Embodiment 2

[0042] (1) Preparation of liquid raw materials containing casein glycomacropeptide

[0043] Take D90 desalted whey powder (protein content 14%, mass fraction; sialic acid content 0.74g / 100g whey powder) and dissolve in 0.02mol / L sodium acetate solution to make the final concentration 100mg / ml. Centrifuge at 500×g for 20 minutes to remove insoluble matter. The supernatant after centrifugation was adjusted to pH 5.2 with glacial acetic acid for later use.

[0044] (2) Treatment of D061 cation exchange resin and 001×7 cation exchange resin

[0045] Take D061 cation exchange resin, swell it in deionized water for 24 hours, soak it with 3 times the volume concentration of 2% hydrochloric acid for 4 hours (intermittent stirring makes the transformation fully), then wash it with deionized water until it is nearly neutral, and then wash it with 3 times the volume of 1mol / L After soaking in sodium hydroxide for 4 hours (intermittent stirring makes the transformation fully), wash with...

Embodiment 3

[0051] (1) Preparation of liquid raw materials containing casein glycomacropeptide

[0052] Take D90 desalted whey powder (protein content 14%, mass fraction; sialic acid content 0.74g / 100g whey powder) and dissolve in 0.02mol / L sodium acetate solution to make the final concentration 100mg / ml. Centrifuge at 500×g for 20 minutes to remove insoluble matter. The supernatant after centrifugation was adjusted to pH 5.4 with glacial acetic acid for later use.

[0053] (2) Treatment of 001×7 cation exchange resin

[0054] Swell the 001×7 cation exchange resin with 0.2mol / L sodium chloride for 5-8 hours, and then gradually add deionized water to swell for 16-19 hours to prevent the resin from absorbing water and bursting. Soak in 3 times the volume concentration of 2% hydrochloric acid for 4 hours (intermittent stirring to make the transformation fully), then wash with deionized water to near neutrality, and then soak in 3 times the volume of 1mol / L sodium hydroxide solution for 4 h...

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Abstract

The invention discloses a method for separating CGMP (casein glycomacropeptide) having high content of sialic acid by utilizing ion exchange resin, aiming to provide a method for acquiring the CGMP having high content of sialic acid, with simple preparation process and low cost. The method comprises the following steps of: adjusting the pH value of a liquid raw material containing the CGMP to be 5.0-5.4, then mixing with cationic exchange resin, sucking out a non-absorbed fluid; adjusting the pH value of the non-absorbed fluid to be 5.0-5.4, then mixing with anion exchange resin, eluting by using sodium chloride after the CGMP is absorbed; or adjusting the pH value of the non-absorbed fluid to be 3.5-4.0, then mixing with the cationic exchange resin, eluting by using sodium chloride after the CGMP is absorbed; carrying out dialysis and desalination on the sodium chloride eluent, and dialyzing an intercept fluid, and acquiring the CGMP having high content of sialic acid after freezing and drying. The complementary technology is alternately is used in the invention, the separation process is reasonably connected, the processing steps are reduced and the sample cost is lowered, so that the CGMP having high content of sialic acid can be acquired.

Description

technical field [0001] The invention relates to the technical field of active substance separation, and more specifically relates to a method for separating casein glycomacropeptide with high sialic acid content by using an ion exchange resin. Background technique [0002] Whey is a by-product of cheese and casein in the industry, in which both lactose and whey protein exist, and its nutrients account for about 55% of raw milk. As the production of cheese increases year by year, there will be a large amount of whey waiting to be utilized and processed every year. Some industrially developed countries already have strong whey processing capacity, but despite this, half of the whey in the world cannot be reused. The discharge of a large amount of whey is not only a waste of resources, but also a serious pollution to the environment. [0003] 80% of the protein in milk is casein. During cheese making, when milk κ-casein (κ-CN) is treated with rennet, κ-CN is hydrolyzed into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/18
Inventor 闫亚丽刁瑞丽陈庆森
Owner TIANJIN UNIV OF COMMERCE
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