Clostridium butyricum and production method of clostridium butyricum feed additive

A feed additive, the technology of Clostridium butyricum, applied in the field of microbial fermentation, can solve the problems of high cost of liquid culture, high requirements for equipment and environment, and achieve the effect of small material loss, simple equipment and low energy consumption

Active Publication Date: 2011-10-19
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The currently reported culture method of Clostridium butyricum is mainly liquid submerged static culture, and its solid-state fermentation culture method h

Method used

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  • Clostridium butyricum and production method of clostridium butyricum feed additive
  • Clostridium butyricum and production method of clostridium butyricum feed additive
  • Clostridium butyricum and production method of clostridium butyricum feed additive

Examples

Experimental program
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Example Embodiment

[0076] Screening Example 7

[0077] Solid-state fermentation medium (%): cottonseed meal hydrolyzed by neutral protease for 1 hour 27; rice bran 9; glucose 1.5; brewer’s yeast powder 2.5; CaCO 3 0.02; water 60.

[0078] Inoculation and culture conditions: inoculum size 10% (v / w), natural pH, culture temperature 37±1°C, culture time 48 hours.

[0079] The number of live bacteria measured was 1.16×10 9 cfu / g.

Example Embodiment

[0080] Screening Example 8

[0081] Solid-state fermentation medium (%): cottonseed meal hydrolyzed for 3 hours by neutral protease 27; rice bran 9; glucose 1.5; beer yeast powder 2.5; CaCO 3 0.02; water 60.

[0082] Inoculation and culture conditions: inoculum size 10% (v / w), natural pH, culture temperature 37±1°C, culture time 48 hours.

[0083] The number of live bacteria measured was 2.25×10 9 cfu / g.

[0084] result

[0085] Clostridium butyricum TK2 uses cheap soybean meal, peanut meal, soybean meal, and cottonseed meal as nitrogen sources. After treatment with neutral protease, its utilization rate has been greatly improved, especially soybean meal after hydrolysis for 3 hours (attached figure 1 ), the effect is remarkable, the number of live bacteria is as high as 4.35×10 9 cfu / g, which greatly reduces the cost for the large-scale production of Clostridium butyricum. Therefore, soybean meal hydrolyzed with neutral protease for 3 hours was selected as the nitrogen...

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Abstract

The invention relates to clostridium butyricum and a production method of a clostridium butyricum feed additive. The name of the clostridium butyricum is TK2, the classification name is Ciostridium butyicum, the preservation number is CGMCC No.4729, the preservation date is April 2, 2011, the preservation address is No. 3, Yard 1, Beichen Western Road, Chaoyang District, Beijing, and the preservation unit is the Chinese General Microbiological Culture Collection Center. The production method disclosed by the invention adopts a solid-state culture method. In the invention, clostridium butyricum TK2 can activate a frozen strain through liquid seed culture, the activated strain is cultured by an optimized solid-state fermentation culture medium, the viable bacterium concentration and the spore concentration of the strain are detected, the viable count of the clostridium butyricum produced by the method is 4.35*109cfu/g, and the spore conversion rate is 85%. Based on the clostridium butyricum, the novel production method for clostridium butyricum biogen with a low cost is developed.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a production method of clostridium butyricum and a clostridium butyricum feed additive. Background technique [0002] Clostridium butyricum is a kind of anaerobic bacilli with endophytic spore structure. It is a normal microorganism beneficial to the host. The preparation made by special technology can inhibit the growth of harmful bacteria through the competitive exclusion of organisms in the digestive tract of animals. , forming dominant flora or preventing diseases by enhancing non-specific immune function, can achieve the purpose of adjusting the micro-ecological balance in the body, promote animal growth and improve feed conversion rate, and reduce the odor of feces. In the context of the increasingly prominent disadvantages of using antibiotics, the many advantages of live Clostridium butyricum preparations make it of great significance in the prevention and treatment of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/145A23K10/18
Inventor 王海宽刘菲菲戚薇
Owner INNER MONGOLIA SCI PLUS BIOTECH
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