Preparation method for producing heparin sodium by using porcine small intestines

A technology of porcine small intestine and heparin sodium, which is applied in the field of preparation of isolated biochemical drugs, can solve the problems of unstable quality of heparin sodium products, low yield of heparin sodium, backward production technology, etc., and achieve the solution of workshop odor, high yield, Potency and Activity Enhanced Effects

Active Publication Date: 2011-11-02
山东绅联药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional production process is backward, the yield of heparin sodium is low, the quality of heparin sodium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0030] (1) Preparation of intestinal mucosa:

[0031] Take 400 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is uniform, and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 420 kg of mucous membrane was collected;

[0032] (2) Enzymolysis:

[0033] Turn on the mucous membrane pump, pump 420 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 3360 kg of water into the enzymolysis tank, turn on the mixer to stir at a speed of 50 r / min, stir evenly, and then add 840 grams of 2709 alkaline protease Add it to the enzymatic hydrolysis tank, adjust the pH...

Embodiment 2

[0046] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0047] (1) Preparation of intestinal mucosa:

[0048] Take 300 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is even and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 310 kg of mucous membrane was collected;

[0049] (2) Enzymolysis:

[0050]Turn on the mucous membrane pump, put 310 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 2790 kg of water into the enzymolysis tank, turn on the mixer and stir at a speed of 55 r / min, stir evenly, then add 620 grams of 2709 alkaline protease Add it to the enzymatic hydrolysis tank, adjust the pH value to...

Embodiment 3

[0063] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0064] (1) Preparation of intestinal mucosa:

[0065] Take 500 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is even and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 520 kg of mucous membrane was collected;

[0066] (2) Enzymolysis:

[0067] Turn on the mucous membrane pump, pump 520 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 3640 kg of water into the enzymolysis tank, turn on the mixer to stir at a speed of 60 r / min, stir evenly, and then add 1040 grams of 2709 alkaline protease Add it to the enzymatic hydrolysis tank, adjust the pH va...

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Abstract

The invention discloses a preparation method for separating biochemical medicaments from mucous membranes of porcine small intestines, in particular a preparation method for producing heparin sodium by using porcine small intestines. The preparation method for producing the heparin sodium by using the porcine small intestines is characterized by comprising the following steps of: preparing intestinal mucosa, and performing enzymolysis, adsorption, elution, precipitation, desalination, dehydration and drying to obtain the heparin sodium. The method has the advantages that: the heparin sodium has high yield, low activity loss and stable quality; the waste liquor is pollution-free in discharge and can be recycled; the problem of stench in a heparin sodium workshop is solved by adopting a workshop waste gas purification treatment device; and 2,000 to 2,400 pieces of porcine small intestines are needed for producing 0.1 billion units of heparin sodium by using the conventional process, only 1,400 to 1,600 pieces of porcine small intestines are needed for producing 0.1 billion units of heparin sodium by using the preparation method, and the effect and the activity of the heparin sodium are greatly improved, so that the production cost is greatly reduced, and the profit can be improved by over 40 percent.

Description

(1) Technical field [0001] The invention relates to a preparation method for isolating biochemical drugs from pig small intestine mucosa, in particular to a preparation method for producing heparin sodium from pig small intestine. (2) Background technology [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation functions...

Claims

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Application Information

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IPC IPC(8): C08B37/10C12S3/02
Inventor 谢成君甄爱华刘建洋
Owner 山东绅联药业股份有限公司
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