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Toxoplasma gondii antibody aptamer with specificity to toxoplasma gondii bacteria and constructed biochip

A biochip, Toxoplasma gondii technology, applied in the field of biomedicine, can solve the problem of high false positive rate

Inactive Publication Date: 2012-12-19
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to statistical data, ELISA and gold standard methods are commonly used in clinical diagnosis in my country to detect serum levels of TOX-IgG and CMV-IgG in pregnant women, and the false positive rate is high, which cannot provide accurate diagnostic basis for clinical use.

Method used

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  • Toxoplasma gondii antibody aptamer with specificity to toxoplasma gondii bacteria and constructed biochip
  • Toxoplasma gondii antibody aptamer with specificity to toxoplasma gondii bacteria and constructed biochip
  • Toxoplasma gondii antibody aptamer with specificity to toxoplasma gondii bacteria and constructed biochip

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0102] The SPR biosensor is provided by Beijing Jinbojia Medical Technology Co., Ltd. The specific instrument model is UMPHO A600 / B, and the biochip is provided by Beijing Jinbojia Medical Technology Co., Ltd. The specific chip model is similar to the UMPHO A600 / B SPR biosensor Matching, including a glass substrate 1, a gold film 2, a connection layer 3, and a surface matrix 4, the glass substrate 1 is sequentially attached with a gold film 2, a connection layer 3 and a surface matrix 4, and there are 4 or 8 on the gold film 2 A flow pool, the flow pool is coated with the aptamers of the sequences of SEQ ID No.1 to SEQ ID No.3. In the process of making the chip, the gold film 2 was pretreated first, that is, soaked and cleaned in 1.0mol / L NaOH for 20 minutes, washed three times with deionized water after taking it out, then put it in Piranha solution for 15 minutes, and immediately put it into a Soak in water and ethanol for 5 minutes, blow dry with nitrogen and set aside. Th...

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Abstract

The invention discloses a toxoplasma gondii antibody aptamer with specificity to toxoplasma gondii bacteria and a biochip constructed by the toxoplasma gondii antibody aptamer. Compared with an antibody used in the conventional detection technology, an oligonucleotide aptamer has the advantages of higher binding force with target molecules, high stability, high accuracy, high repeatability, reusability, long-time storability, accurate locus modification, shorter screening period and the like. When the obtained biochip is put into SPR (Surface Plasmon Resonance) biosensor detection, real-time detection, no need of marking samples, extremely small sample demand quantity, convenient and quick detection process and high sensitivity, very wide application range, high flux and detection of turbid and even nontransparent samples in most cases can be realized.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a toxoplasma antibody aptamer specific to toxoplasma virus and a biochip capable of detecting toxoplasma infection in the early stage of pregnant women. Background technique [0002] Birth defects bring great mental and economic burdens to families, countries and society. At present, there is no effective treatment for fetal congenital defects. Among the methods of preventing birth defects, the primary prevention in the first trimester is the most effective. Toxoplasma gondii is a group of pathogenic microorganisms infected during pregnancy, which can cause intrauterine infection through the placenta or birth canal, leading to miscarriage, stillbirth or fetal growth retardation, congenital malformations, neonatal infection and growth and development disorders of infants and young children. About 26,000 children with toxoplasma virus infection are born in my country every y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115C12N15/10C12Q1/68C12Q1/04
Inventor 刘星府伟灵姚春艳
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA