Microfluidic control chip for realizing PCR (Polymerase Chain Reaction) and real-time PCR virus quick detection device

A microfluidic chip, microfluidic channel technology, applied in enzymology/microbiology devices, biochemical cleaning devices, bioreactors/fermenters for specific purposes, etc. The problems of large amount and high sample consumption can achieve the effect of less sample consumption, fast detection speed and integrated operation.
CN102286358BInactive Publication Date: 2013-06-19HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
Publication Date
2013-06-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a microfluidic control chip which is formed by bonding and sealing PDMS (Polydimethylsiloxane) material integrated with a micro valve, and comprises an upper layer of air control passage, a middle layer of PDMS membrane and a lower layer of microfluidic passage. A real-time PCR virus quick detection device comprises the microfluidic control chip, a temperature control unit, a signal detection unit and a data processing and control unit. The invention designs the microfluidic control chip which is integrated with the micro valve, is in a three-layer PDMS structure, andis used for virus detection and the real-time fluorescent PCR detection device. Compared with the traditional method, the invention has the advantages of short time, less sample usage, quick detection speed, simpleness and convenience in operation, integration and the like.
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Description

technical field

[0001] The invention relates to a microfluidic chip for realizing polymerase chain reaction (Polymerase Chain Reaction, PCR) and a virus rapid detection device for real-time PCR. Background technique

[0002] Since the concept of micro-total analysis system was proposed in 1990, microfluidic chip technology has opened up a broad space for development in the fields of chemistry, life science, environmental science and food science. Based on microfluidic control technology, microfluidic chip technology guides the development of analytical instruments in the direction of miniaturization, integration, automation, and high-throughput rapid quantitative analysis, providing a possibility for real-time detection and on-site analysis of biochemical environmental samples. strategy, which has broad market prospects in medical diagnosis and epidemic monitoring in agricultural breeding systems.

[0003] Polymerase chain reaction (Polymease Chain Reaction, PCR), also know...

Claims

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