Method for quickly obtaining capsicum transgenic plant

Abstract

The invention relates to a method for quickly obtaining a capsicum transgenic plant, which comprises the following steps: sampling; activating and infecting agrobacterium; preparing a regeneration culture medium and a screening culture medium; inoculating the immature embryo, and culturing; and forming adventitious bud and regenerating the plant, thereby efficiently obtaining the capsicum transgenic regenerated plant. In the invention, it usually takes 7 weeks from the immature embryo infected by agrobacterium to the acquisition of the regenerated plant, the formation rate of the regenerated plant in the culture process is up to 50% to the maximum, and the positive rate of the transgenic plant is up to 80%. In the invention, the immature embryo is used as a receptor material to infect theagrobacterium and carry out inoculation and culture so as to directly form the regenerated plant, thereby shortening the culture period and enhancing the culture effect and genetic transformation efficiency. If applied to genetic functional verification and breeding practice, the invention can greatly accelerate the research into capsicum functional genomics and the development of breeding practice.

Description

Technical field [0001] The present invention involves the method of obtaining genetic plants in the field of biotechnology, especially a method to quickly obtain genetically modified plants through agricultural invasion of pepper immature embryos. Background technique [0002] GM technology is the use of reorganization DNA, cyt tissue culture or gear system transformation, to import the exogenous gene into plant cells or tissues, so that it is directed to reorganize genetic materials, improve plant traits, and cultivate new varieties of high -quality high -yield crops.Since the birth of genetically modified plants in 1983, natural plant genetic engineering has begun to set the genetic traits of improved plants under manual control.Compared with conventional breeding methods, it has the following characteristics: 1) unlimited restrictions, which can realize the exchange of genetic substances in animals, plants and microorganisms, so as to make full use of the various genetic resou...

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Problems solved by technology

However, pepper tissue culture has strong genotype specificity, and the regeneration efficiency is not ideal. In most experiments, the regeneration cycle of the plants is long or the differentiation frequency is low, and the growth ability of the adventitious buds is poor, and some even stay in the bud induction stage and die. cannot grow further, which largely limits the research progress of pepper genetic engineering

At present, the research on pepper transgenic work mainly focuses on the establishment of genetic transformation system, but there are still some major problems in the genetic transformation process that limit the application of pepper transgenic technology in actual breeding, such as: long cultivation period, difficult plant regeneration and genetic transformation rate inferior

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