A method for simultaneous fermentation of cassava residues to produce ethanol and hydrogen

A cassava residue and hydrogen technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as environmental pollution in the cassava starch industry, achieve great demonstration and promotion value, reduce production costs, and solve environmental pollution. effect of the problem

Inactive Publication Date: 2011-12-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned deficiencies existing in the prior art, and to provide a method for producing energy alcohol and hydrogen by fermenting cassava dregs, which uses cassava dregs as raw materials and not onl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] The method of using cassava residue to produce ethanol and hydrogen simultaneously by fermentation is as follows:

[0025] (1) Preparation of thermophilic anaerobic bacteria seed solution: preserved at -80℃ Clostridium thermocellum Manufactured by 10mL vial activation and 100mL serum vial amplification, cultured for 18 hours OD 600 ~0.6 seed liquid; the medium is MTC medium, the main components of the medium are A, B, C, D, E liquid, and the 5 components are according to 45:2:1:1:1 ( V / V ) Mix with a sterile syringe. Liquid A is 3 g / L cellulose. Liquid B: Tripotassium citrate 50 g / L, citric acid monohydrate 31.25 g / L, sodium sulfate 25 g / L, potassium dihydrogen phosphate 25 g / L, sodium bicarbonate 62.5 g / L; C liquid: chlorine Ammonium chloride 75 g / L, urea 250 g / L, yeast extract 50 g / L; D solution: magnesium chloride hexahydrate 50 g / L, ferrous chloride tetrahydrate 5 g / L, calcium chloride dihydrate 10 g / L, monohydrate cysteine ​​hydrochloride 50 g / ...

Example Embodiment

[0031] Example 2

[0032] The method of using cassava residue to produce ethanol and hydrogen simultaneously by fermentation is as follows:

[0033] (1) Preparation of thermophilic anaerobic bacteria seed solution: preserved at -20℃ Thermoanaerobacterium aotearoense Activated by 10mL vial and amplified by 100mL serum vial, cultured for 24 hours OD 600 ~0.8 seed liquid; the medium is MTC medium, the main components of the medium are A, B, C, D, E liquid, and the 5 components are according to 45:2:1:1:1 ( V / V ) Mix with a sterile syringe. Liquid A is 5 g / L xylose. Liquid B: Tripotassium citrate 50 g / L, citric acid monohydrate 31.25 g / L, sodium sulfate 25 g / L, potassium dihydrogen phosphate 25 g / L, sodium bicarbonate 62.5 g / L; C liquid: chlorine Ammonium chloride 75 g / L, urea 250 g / L, yeast extract 50 g / L; D solution: magnesium chloride hexahydrate 50 g / L, ferrous chloride tetrahydrate 5 g / L, calcium chloride dihydrate 10 g / L, monohydrate cysteine ​​hydrochloride 50 g / L; E liq...

Example Embodiment

[0039] Example 3

[0040] The method of using cassava residue to produce ethanol and hydrogen simultaneously by fermentation is as follows:

[0041] (1) Preparation of thermophilic anaerobe seed liquid: preserved at -196℃ Clostridium thermocellum with Thermoanaerobacterium aotearoense After 10mL vial activation and 100mL serum vial amplification, the yellow substance was obtained after 48 hours of culture. Clostridium thermocellum Seed liquid and OD 600 ~1.1 Thermoanaerobacterium aotearoense Seed liquid; the medium is MTC medium, the main components of the medium are A, B, C, D, E liquid, and the 5 components are according to 45:2:1:1:1 ( V / V ) Mix with a sterile syringe. Strain Clostridium thermocellum with Thermoanaerobacterium aotearoense The liquid A is 3 g / L cellulose or 5 g / L xylose. Liquid B: Tripotassium citrate 50 g / L, citric acid monohydrate 31.25 g / L, sodium sulfate 25 g / L, potassium dihydrogen phosphate 25 g / L, sodium bicarbonate 62.5 g / L; C liquid: chlorine Am...

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Abstract

The invention discloses a method for simultaneously producing ethanol and hydrogen by using cassava residue through fermentation. The method comprises the following steps of: preparing two kinds of thermo-anaerobes and yeast seed liquor; preparing a fermentation medium and sterilizing; performing thermophilic fermentation and collecting the hydrogen; and performing ethanol fermentation and the like. In the integrated fermentation process, after the thermophilic fermentation is performed for 72 to 108 hours, yeast is inoculated, fermentation is continued for 12 to 24 hours, fermented liquor has the alcohol content of more than 7.12g/L, the fermentation efficiency is over 75 percent and the fermentation is stopped. The cassava residue is a waste in the cassava starch industry, contains fine-particle starch and cellulose, has a larger specific surface area, is easily hydrolyzed and utilized by enzyme producing microbes without pretreatment, can be used for integrated fermentation of the ethanol and the hydrogen without additional enzyme, has the characteristic of negative cost, can be changed into treasure, and solves the problem of environmental pollution in the cassava starch industry.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a method of using cassava residue, a waste of the cassava starch industry, as a raw material, using two thermophilic bacteria to co-cultivate hydrogen and hydrolyze the raw material, and then using yeast to rapidly ferment the method for producing alcohol. Background technique [0002] The gradual depletion of fossil energy requires us to develop alternative, sustainable and clean new energy sources. In recent years, the production of hydrogen and fuel alcohol has also been increasing. Using various biomass and industrial and agricultural wastes as raw materials is considered to be an effective way to reduce the cost of new energy and replace traditional energy. However, at present, most energy alcohol is produced from cornstarch or sugar crops, which leads to food shortage and price rise, and the cost of raw materials is very high, which hinders the development o...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P5/02C12P7/10C12R1/145C12R1/01C12R1/865
CPCY02E50/16Y02E50/10
Inventor 朱明军李平
Owner SOUTH CHINA UNIV OF TECH
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