Method for extracting daphnoretin from wikstroemia indica

Abstract

The present invention discloses a method for extracting daphnoretin from wikstroemia indica. The method comprises steps of enzymolysis, abstraction, extraction, separation and recrystallization, and concretely comprises that: crushing bark of the wikstroemia indica, adding cellulose to the crushed bark and stirring; adding distilled water to the resulting mixture to carry out soaking for 24 hours; carrying out suction filtration and drying to obtain enzymatic bark powder; then carrying out abstraction through methanol or ethanol; carrying out concentration to obtain the extract; dissolving the extract in methanol, then carrying out extraction through dichloromethane or chloroform; carrying out elution for the extractant through a mixed solution comprising petroleum ether and acetic ether,then carrying out crystallization to obtain the pure daphnoretin, wherein the purity of the daphnoretin is more than 99%. The daphnoretin separated through the preparation method provided by the present invention has high purity and high yield; the operation of the preparation method is simple; the process of the preparation method is simple; the raw materials can be recycled; the separation efficiency is high; the method is applicable for the industrial production on a large scale.

Description

technical field [0001] The invention relates to the technical field of chemical industry and medicine, in particular to a method for separating siratin from Liaogewang. Background technique [0002] Brother Wang [ Wikstroemia indica (L.) C. A. Mey] is a plant of the genus Paphelia in the family Daphneaceae. It is also known as Nanling Padma, Gossypium, Gechunguang, and Sparrow. It is widely distributed in Jiangxi, Guangdong, Guangxi and other provinces and regions in my country, and is rich in resources. The roots, stems, leaves, and fruits of Ligewanga can be used as medicine. It is bitter and cold in nature and slightly pungent. Modern pharmacological and clinical studies have shown that Liaogewang has antibacterial, antiviral, anti-inflammatory, analgesic, anti-cancer, anti-malarial effects, and is used to treat bronchitis, pneumonia, tonsillitis, mastitis, cellulitis and other diseases. Liaogewang contains many compounds such as flavonoids and coumarins, such as genkw...

AI Technical Summary

Problems solved by technology

Although some authors have obtained pure sirutinin through the systematic separation and structural identification of the chemical components of Gewang, and some authors have optimized the extraction process of Gewang by using sirutin as an investigation index, but these methods are cumbersome and require a large amount of organic solvents , large-scale equipment cannot be realized, so it only stays at the research level of the laboratory, and cannot be applied in large-scale production, and it is also impossible to obtain a large amount of pure sirupin