Real-time reverse transcription-polymerase chain reaction (RT-PCR) detection method and kit for Coxsackie virus
A coxsackie virus and kit technology, applied in the field of molecular biology and nucleic acid detection, can solve the problem of limited group resolution, lack of serotype coxsackie virus high sensitivity, high specificity, flexible and simple detection method, poor sensitivity And other issues
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[0116] Example 1
[0117] 1Materials and methods
[0118] 1.1 Materials and reagents
[0119] Inactivated Coxsackie virus A9, A16, B2, B3 and B5 and GII-containing norovirus fecal samples were purchased from the Institute of Virus Prevention and Control of the Chinese Center for Disease Control and Prevention. Serum I, II and III polioviruses were inactivated The live vaccine strain suspension was obtained from the University of California.
[0120] TIANamp virus RNA extraction kit: Tiangen Biotechnology Co., Ltd., Cat.No.SD101; Prime Script TM Reverse transcription kit: Bao Biological Engineering Co., Ltd., Cat.No.DRR037A; Premix Ex Taq TM Real-time fluorescence amplification kit: Bao Biological Engineering Co., Ltd., Cat.No.DRR039A.
[0121] 1.2 Primers and probes
[0122] Search the Coxsackie virus A9, A16, B2, B3, and B5 cDNA sequences in the GenBank database, and compare and analyze them to find that the serotypes are relatively conservative, but are compared with other serotypes...
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