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Laccase fermentation and separation coupled system and method

A laccase and separation device technology is applied in the system field of laccase fermentation and separation coupling, and can solve the problems of complicated procedures, low expression level of laccase, complicated separation and purification operations, and the like

Inactive Publication Date: 2013-06-05
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the problems that the current laccase fermentation and separation and purification operations are carried out separately, the procedures are cumbersome, the fermentation and separation have low laccase expression levels, and the separation and purification operations are complicated. Therefore, the purpose of the present invention is to provide a laccase fermentation and separation coupling system

Method used

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  • Laccase fermentation and separation coupled system and method
  • Laccase fermentation and separation coupled system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Using the methods reported in existing literature (Dominguez A, Gomez J, Lorenzo M, Sanroman A.World J.Microbiol.Biotechnol, 2007, 23, 367), the preservation number is prepared from Trametes versicolor (Trametes versicolor) with the preservation number CICC 14001 into the immobilized Trametes versicolor with calcium alginate gel as the carrier;

[0068] 2. Put the immobilized Trametes versicolor into the bubbling bed reactor 1 according to the loading capacity of 20% (v / v), the liquid loading capacity of the medium is 65% (v / v), the culture temperature is 25°C, and the ventilation rate is 0.2 vvm, the pH value is controlled at 4.0; the composition of the fermentation medium (g / L): KH 2 PO 4 0.2, CaCl 2 2H 2 O 0.1, MgSO 4 ·7H 2 O 0.05, NH 4 h 2 PO 4 0.5, FeSO 4 ·7H 2 O 0.035, glucose 2, NH 4 Cl 0.267, CuSO 4 ·5H 2 O 0.02;

[0069] 3. Preparation of superparamagnetic silica mesoporous nanoparticle carrier with a pore size of 18.3 nm,

[0070] The prepara...

Embodiment 2

[0081] 1. Using the methods reported in existing literature (Dominguez A, Gomez J, Lorenzo M, Sanroman A.World J.Microbiol.Biotechnol, 2007, 23, 367), the preservation number is prepared from Trametes versicolor (Trametes versicolor) with the preservation number CICC 14001 into the immobilized Trametes versicolor with calcium alginate gel as the carrier;

[0082] 2. Put the immobilized Trametes versicolor into the bubbling bed reactor 1 with a loading capacity of 15% (v / v), the medium loading volume is 70% (v / v), the culture temperature is 25°C, and the ventilation rate is 0.15 vvm, the pH value is controlled at 4.0; The composition of fermentation medium is the same as embodiment 1;

[0083] 3. Preparation of superparamagnetic silica mesoporous nanoparticle carrier with a pore size of 18.3 nm,

[0084] The concrete method of preparation is with embodiment 1;

[0085] 4. Wash the superparamagnetic silica mesoporous nanoparticle carrier chelated with copper ions for more than...

Embodiment 3

[0090] 1. Using the methods reported in existing literature (Dominguez A, Gomez J, Lorenzo M, Sanroman A.World J.Microbiol.Biotechnol, 2007, 23, 367), the preservation number is prepared from Trametes versicolor (Trametes versicolor) with the preservation number CICC 14001 into the immobilized Trametes versicolor with calcium alginate gel as the carrier;

[0091] 2. Put the immobilized Trametes versicolor into the bubbling bed reactor with a loading capacity of 10% (v / v), the medium loading volume is 75% (v / v), the culture temperature is 25°C, and the ventilation rate is 0.1vvm , the pH value is controlled at 4.0;

[0092] 3. Preparation of superparamagnetic silica mesoporous nanoparticle carrier with a pore size of 18.3 nm,

[0093] The concrete method of preparation is with embodiment 1;

[0094] 4. After washing the superparamagnetic silica mesoporous nanoparticle carrier chelated with copper ions for more than 3 times successively with ethanol and sterile water, put it i...

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Abstract

The invention relates to the technical field of biological engineering, specifically to a laccase fermentation and separation coupled system and a method thereof. The laccase fermentation and separation coupled system provided by the invention comprises a bubbling bed reactor, a magnetic medium particle in situ continuous separating device and a peristaltic pump, wherein the top and bottom of thebubbling bed reactor respectively communicate with a feed inlet and a discharge hatch of the magnetic medium particle in situ continuous separating device through the peristaltic pump. The laccase fermentation and separation coupled method provided by the invention comprises the following steps of: placing the immobilized laccase strain into the bubbling bed reactor for fermentation, introducing a fermentation broth by the peristaltic pump into the magnetic medium particle in situ continuous separating device for selective separation, and allowing the separated fermentation broth to return the bubbling bed reactor under the action of the peristaltic pump for continuous fermentation. The system and method provided by the invention are used to change the traditional laccase production mode and raise the fermentation and separation level, are simple to operate and are convenient to amplify.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a system and method for coupling laccase fermentation and separation. Background technique [0002] Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) is a copper-containing polyphenol oxidase, which belongs to the same blue multi-copper oxidase as ascorbate oxidase in plants and ceruloplasmin in mammals family. Laccase can catalyze O 2 Reduction to water via 4 electrons is accompanied by oxidation of some phenolic substrates. Laccase is mainly used in organic synthesis, degradation of environmental pollutants and dye decolorization, biological detection and electrochemical analysis, pulp bleaching and wastewater treatment in the paper industry, degradation of fiber raw materials, etc. It is precisely because of the broad application prospects of laccases that in recent decades, laccases have become a research hotspot in biology, chemistry and environmental scien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/02C12N9/02C12R1/01
CPCC12M25/20C12M21/18C12M47/10C12M27/02C12M21/14
Inventor 刘春朝王锋郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI