Method for evaluating and predicating toxicity and efficacy of medicament by using metabonomic technology
A metabolomics and metabolite technology, which is applied in the field of application of metabolomics technology to evaluate and predict drug toxicity and efficacy, and can solve problems such as the elimination of exogenous metabolite signals.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0087] In order to realize the drug efficacy and toxicity evaluation of the present invention, it is necessary to eliminate the chromatographic baseline drift of one of the two types of interference signals included in the signal matrix obtained by HPLC-MS / MS detection of biological samples, and the present invention proposes an elimination method. In order to prove the feasibility of the method, a standard sample system was designed in this embodiment, and at the same time, a CCl4-induced hepatotoxic SD rat urine sample signal was used for verification, which is now explained as follows:
[0088] Standard sample system: for the chromatograms of three mixed standard samples such as blank control group, drug administration group and exogenous substances (attached figure 1 ), follow the steps below to eliminate chromatographic baseline drift:
[0089] I) In the signal matrix with the retention time Rt and the mass-to-charge ratio m / z as the row-column index or column-row index, ...
Embodiment 2
[0095] In order to realize the drug efficacy and toxicity evaluation of the present invention, it is necessary to eliminate the exogenous metabolite signal, one of the two types of interference signals included in the signal matrix obtained by HPLC-MS / MS detection of biological samples, the present invention proposes two a method of elimination. In order to prove the feasibility of the method, the standard sample system of the system described in Example 1 was used in this embodiment, and at the same time, another simulation system was designed for verification, which is now described as follows:
[0096] Simulation system: see attached Figure 6 , the figure shows that endogenous and exogenous metabolites have obvious signal overlap, which is difficult to eliminate by common methods. Utilize the method for eliminating exogenous metabolite signals described in the summary of the invention—"mass spectrometry signal orthogonal projection method" to obtain the endogenous metabol...
Embodiment 3
[0107] The experimental SD rats were randomly divided into the blank control group and the xanthate subgroup, and the water extract of the Chinese medicine xanthate subgroup was administered to the rats (60g / kg) repeatedly, for 3 consecutive days, twice a day, and the collection time was 0-24h. , 24h~48h, 48h~72h urine; the normal group was given corresponding doses of distilled water, and collected 0~24h, 24h~48h, 48h~72h urine for 3 consecutive days; urine samples were precipitated with methanol to remove protein, and centrifuged at 12000rpm After 5 minutes, the supernatant was filtered through a 0.45 μm microporous membrane, and the sample measurement signal matrix was obtained by HPLC-MS / MS (the retention time Rt is the row index, and the mass-to-charge ratio m / z is the column index). Three days after administration, the xanthate subgroup and the normal control group were anesthetized by intraperitoneal injection of 4% chloral hydrate (1ml / 100g body weight), blood samples w...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com