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Extraction method of ginkgo leaf flavonoids based on steam explosion

A technology of ginkgo flavonoids and ginkgo leaves, which is applied in the chemical industry, can solve the problems of limited influence on the fine structure of ginkgo leaves, a decrease in the specific surface area of ​​ginkgo leaves, and unfavorable large-scale production, and achieve improved extraction yield, low cost, and economical production cost effect

Inactive Publication Date: 2012-02-15
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The organic solvent method is currently the most widely used extraction method in China. It is mainly divided into cold soaking method, percolation method and reflux method. Due to its high cost, long time-consuming and low efficiency, the content of impurities is high and the amount of solvent used is large. cumbersome operation
Ultrasonic technology can improve the yield of ginkgo flavonoids, but because ultrasonic is easy to produce cavitation, it has limited influence on the fine structure of ginkgo leaves and causes a decrease in the specific surface area of ​​ginkgo leaves, which is not conducive to large-scale industrial production
The supercritical extraction method has disadvantages such as long operation time and high consumption of organic solvents.

Method used

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  • Extraction method of ginkgo leaf flavonoids based on steam explosion
  • Extraction method of ginkgo leaf flavonoids based on steam explosion
  • Extraction method of ginkgo leaf flavonoids based on steam explosion

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Take 100g ginkgo with a particle size of 20 mesh (the SEM picture is shown in 1), add 15 times the mass of ginkgo with a volume percentage of 60% ethanol solution, at 60°C, the pH value is 7 (pH7-9 is acceptable) Under the condition of reflux extraction reaction for 4 hours, the reaction solution was obtained, and the reaction solution was concentrated in a rotary vacuum at 70°C, and the liquid was taken, and the liquid was a ginkgo flavonoid solution; the obtained solution was pre-prepared at -18°C Frozen for 4.5 hours, then placed in a freeze dryer, and freeze-dried for 30 hours at a vacuum degree of 45Pa and a temperature of -55°C to obtain 9.6 g of ginkgo flavonoids, and the yield of ginkgo flavonoids was 30.14%.

Embodiment 2

[0025] Weigh 100g of ginkgo with a particle size of 20 mesh and put it into a three-neck bottle, then add 500mL of aqueous solution, and ultrasonically react for 1.5h with an ultrasonic power of 350w. After the reaction is completed, wash and filter with distilled water until the filtrate is neutral. The filter residue was vacuum-dried at 70°C for later use. Take 100g ginkgo with a particle size of 20 mesh, add 15 times the mass of ginkgo with a volume percentage of 60% ethanol solution, and carry out reflux extraction reaction at 60°C with a pH value of 9 (pH7-9 is acceptable) for 4 hours to obtain the reaction solution, the reaction solution was concentrated in a rotary vacuum at 70°C, and the liquid was taken, which was a ginkgo flavonoid solution; the resulting solution was pre-frozen at -18°C for 4.5 hours, and then placed in a freeze dryer , in a vacuum of 45Pa and a temperature of -55°C, after freeze-drying for 30 hours, the quality of ginkgo flavonoids was 11.7g, and t...

Embodiment 3

[0027] Weigh 100g of ginkgo with a particle size of 20 mesh and put it into a three-necked bottle, then add 500mL of aqueous solution, and microwave at 400w for 1min. After the reaction, wash and filter with distilled water until the filtrate is neutral. The filter residue was vacuum-dried at 70°C for later use. Take 100 g of ginkgo with a particle size of 20 mesh, add an ethanol solution equivalent to 15 times the mass of ginkgo with a volume percentage of 60%, and carry out reflux extraction reaction at 60°C for 4 hours to obtain a reaction solution, which is heated at 70°C Carry out rotary vacuum concentration under the conditions, take the liquid, and the liquid is ginkgo flavonoid solution; pre-freeze the obtained solution at -18°C for 4.5 hours, and then place it in a freeze dryer, under 45Pa vacuum degree, -55°C temperature After freeze-drying for 30 hours, the quality of ginkgo flavonoids obtained was 12.9 g, and the yield of ginkgo flavonoids was 40.50%.

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Abstract

The invention relates to an extraction method of active ingredients in ginkgo and discloses a preparation process for extracting ginkgo flavonoids by steam explosion pre-treatment of ginkgo leaves according to the invention. The extraction method comprises the following steps: cleaning, drying, soaking overnight, performing steam explosion treatment, filtering, drying and extracting the ginkgo flavonoids with ethanol. The method is simple in equipment and easy to operate, and can greatly improve the extraction rate of total flavonoids from the ginkgo leaves; simultaneously, byproducts generated during the production process have no pollution, and the used ethanol and purified water can be recycled, thereby further reducing the production cost; and products prepared by adopting the method provided by the invention can be widely used in medicine and health care product industries.

Description

technical field [0001] The invention relates to the field of chemical industry, in particular to a process for extracting ginkgo flavonoids from ginkgo leaves. Background technique [0002] Ginkgo tree is an ancient tree species in my country and is known as "the living fossil on the earth". The amount owned by my country accounts for more than 70% of the world's total. Ginkgo biloba has been used as a medicine for heart and lung diseases in my country for thousands of years. In 1966, German scientists discovered that Ginkgo biloba contains effective ingredients for lowering cholesterol, and since then began modern research on the pharmacology and application of Ginkgo biloba. Ginkgo biloba extract contains a variety of active substances, such as flavonoids, terpene lactones, etc., which have medical and health effects such as improving cardiovascular and cerebrovascular circulation, anti-virus, anti-cancer, anti-aging and lowering cholesterol, and have less adverse reactio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
Inventor 张兵兵曾国明张茂兰徐洪记
Owner CHONGQING UNIV
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