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Borojo active extract and preparation method and antibacterial and anti-oxidation application thereof

An extract and active technology, applied in the field of plant active ingredients, can solve problems such as no reports to clarify active ingredients and principles.

Active Publication Date: 2012-02-15
梁公壁 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report to clarify its active ingredients and principle

Method used

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  • Borojo active extract and preparation method and antibacterial and anti-oxidation application thereof
  • Borojo active extract and preparation method and antibacterial and anti-oxidation application thereof
  • Borojo active extract and preparation method and antibacterial and anti-oxidation application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Active substance crystallization purification semi-preparation scheme

[0028] Sample and quantity: Borojo pulp sample 200 grams.

[0029] Solvent and ratio: water: methanol = 1:1 mixture.

[0030] Instrument and its conditions: Hanbon Technology Analytical Chromatograph, model: Newstyle, manufacturer: Jiangsu Hanbon Technology UV detector wavelength 254.

[0031] Industrial purification equipment: industrial semi-preparative chromatography system NP7010C preparation pump, model: NP7010C, manufacturer: Hanbon Technology; separation equipment (can directly use the methodology of NP7010C), model: Prep-300, manufacturer: Hanbon Technology.

[0032] Crystallization purification preparation steps and results:

[0033] 1. Remove the seeds from Borojo pulp and mix evenly;

[0034] 2. Add 1:1 water and methanol mixture, mix and shake evenly;

[0035] 3. Heat to 60-75°C, and extract a yellow concentrated liquid through a rotary evaporator;

[0036] 4. Elute with a...

Embodiment 2

[0039] The molecular structure of embodiment 2 active substance purification is determined

[0040] Basic conditions for molecular structure determination:

[0041] Example 1 semi-prepared crystalline material (6ml) is used for the sample of carbon nuclear magnetic resonance spectrum and proton spectrum analysis

[0042] ●Instruments used:

[0043] 1. NMR spectrometer, model: Avance 500: manufacturer: Bruker A.G., Switzerland

[0044] 2. Fourier infrared-Raman-microscopic spectrometer (FT-IP), model: Nexus 670; manufacturer: Nicolet Corporation of the United States,

[0045] ●Compound structure software: Use ChemDraw Ultra to deduce the hydrogen spectrum and carbon spectrum structure of the active substance, and get figure 1 , image 3 .

[0046] Structure analysis:

[0047] One, the result of nuclear magnetic resonance instrument proton spectrum (add deuterated methanol), see figure 1 , figure 2 .

[0048] H NMR spectrum figure 1 , 2 From the point of view, the the...

Embodiment 3

[0062] Determination of active substance content in embodiment 3 Borojo fruit and its products-HPLC-MS / MS method

[0063] 1. Safety Tips

[0064] Flammable and toxic chemicals used in this standard operating procedure, such as methanol, should avoid inhalation of its vapor or direct contact with the skin. When handling such substances, it can be operated in a fume hood, and protective gloves, protective glasses or anti-virus can be worn if necessary. mask.

[0065] 2 method summary

[0066] This standard operating procedure is formulated by our laboratory by referring to the sample pretreatment method and measurement method in the relevant literature, after careful verification, and refining some key steps.

[0067] This standard operating procedure is applicable to the determination of active substances in Borojo original jam and its products.

[0068] 3 principles

[0069] The active substance in the sample is extracted with a suitable pretreatment method, enters a high-...

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Abstract

The invention provides a South American fruit Borojo active extract and an identification and preparation method, a content measuring method and application thereof. The Borojo active extract has natural anti-oxidation, antiracial and antifungal effects.

Description

technical field [0001] The invention relates to plant active ingredients and uses, in particular to active extracts of Borojo and their preparation methods and uses. Background technique [0002] Borojo (Borojo) is a fruit that grows in the tropical rainforest of South America. It is the only one adapted to the climate of Ecuador. It belongs to the genus Rubiaceae. There are two varieties, Borojoa patinoi Cuatrec and Borojoa sorbilis Cuatrec. Among the locals, there are people who eat Borojoa sorbilis Cuatrec Habit. The diameter of Borojo fruit is 7-12 cm. It is green when immature, and turns to tea brown when mature. The weight is 700-1000 grams per fruit. The flesh is soft, sour and sticky. After removing the seeds, the flesh accounts for 88% of the weight. [0003] Fresh Borojo is extremely acidic (pH2.74), low in moisture (69.22%), high in carbohydrates (28.02%), low in sugar (9.72%), high in protein (1.22%), and high in dietary fiber (2.98%). B of the B vitamins 1 、B...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61P39/06A61P31/04A23L1/29A23L33/00
Inventor 梁公壁梁志强焦红潘滔吕伟雄黄健豪
Owner 梁公壁
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