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Pulmonic targeting immuno nano liposome and preparation method thereof

A nano-liposome and lung-targeting technology, applied in the field of medicine, can solve problems affecting the compliance of glucocorticoid drugs, delay wound healing, aggravate infection, etc., to reduce systemic adverse reactions, new clinical practical value, reduce The effect of dosage

Inactive Publication Date: 2012-03-14
SHANGHAI PULMONARY HOSPITAL AFFILIATED TO TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glucocorticoids are a class of drugs with very obvious adverse reactions. Long-term application or high-dose application can often lead to a series of adverse reactions, such as hypertension, elevated blood sugar, peptic ulcer, gastrointestinal bleeding, Cushing syndrome, etc. symptoms, osteoporosis, aseptic bone necrosis, fractures, delayed wound healing, induced or aggravated infection, growth retardation in children, etc., which can be disabled or life-threatening in severe cases
It is precisely because of this "double-edged sword" effect that seriously affects the clinical efficacy and treatment compliance of glucocorticoids

Method used

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  • Pulmonic targeting immuno nano liposome and preparation method thereof
  • Pulmonic targeting immuno nano liposome and preparation method thereof
  • Pulmonic targeting immuno nano liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Preparation of dexamethasone nanoliposomes (NLP)

[0058] Dexamethasone nanoliposomes were prepared by thin film dispersion method. Accurately weigh the prescribed amount of soybean lecithin, cholesterol, distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000) (molar ratio 1.8:1:0.2), dissolve in chloroform, and place in a circular bottom flask, 50 0 C water bath, 120r·min -1 The organic solvent was removed by rotary evaporation under reduced pressure, and a uniform transparent film was formed on the wall of the flask. 37 0 C dried in vacuo overnight. Precisely weigh the prescription amount of dexamethasone sodium phosphate (the ratio of DXM to phospholipid is 1 mg DXM:10 μmol phospholipid), dissolve it in normal saline with the same volume as chloroform, and place it in a film-forming round bottom flask, 50 0 C water bath, 120r·min -1 Spin for 2 h to hydrate and dry the lipid film. The prepared suspension was allowed to stand at room temper...

Embodiment 2

[0076] Tissue-targeting Experiment of Pulmonary Surfactant Protein A Polyclonal Antibody Conjugated to Dexamethasone Immune Nanoliposomes

[0077] 105 healthy male SD rats, weighing (98±12) g, were randomly divided into 3 groups: pulmonary surfactant protein A polyclonal antibody conjugated dexamethasone immune nanoliposome (SPA-NLP) group, dexamethasone nano Liposome (NLP) group and dexamethasone (DXM) group. (1) SPA-NLP group was injected with SPA-NLP (based on dexamethasone sodium phosphate 2mg / Kg body weight) through the tail vein of rats; (2) NLP was injected through the tail vein of rats in the dexamethasone nanoliposome group Methasone sodium phosphate 2mg / Kg body weight); (3) The dexamethasone group was injected with dexamethasone sodium phosphate 2mg / Kg through the tail vein of rats. Take 15min, 30min, 1h, 2h, 4h, 8h, 12h after administration as time points. At each time point, 5 rats were taken from each of the three groups. After ether anesthesia, blood was collec...

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Abstract

The invention, belonging to the technical field of medicament, relates to an immuno liposome with pulmonic target activity and a preparation method thereof. The immuno liposome disclosed herein comprises pulmonic surfactant protein (SP) A polyclonal antibody, actie pharmaceutical ingredients and a nano liposome, wherein, dexamethasone sodium phosphate (DXM) and other glucocorticoids are used as the actie pharmaceutical ingredients, the nano liposome is used as a carrier, and the SP-A polyclonal antibody is used as a specific pulmonic targeted agent. The immuno nano liposome disclosed herein has the advantages of definite pulmonic target activity, and efficient and stable realization of the targeted conveying of the actie pharmaceutical ingredients to lung, so that maximum concentration ofDXM in the lung is increased by 5.08 times compared with common medicines, and the area under curve when 12 hours after injecting the medicine is increased by 40.21 times. According to the invention,the dosage of glucocorticoids can be reduced, the curative effect on lung diseases can be improved, and systematic side effect can be reduced at the same time. The invention has new values for clinicapplication.

Description

technical field [0001] The invention belongs to the technical field of medicine. The invention relates to a lung-targeted immune liposome, in particular to a lung-targeted dexamethasone immune nano-liposome and a preparation method thereof. Background technique [0002] The prior art discloses that glucocorticoid drugs have strong anti-inflammatory and immunosuppressive effects, and have been widely used in the treatment of inflammatory and immune diseases in various systems of the body. Such drugs are also widely used in the treatment of respiratory diseases, such as bronchial asthma, chronic obstructive pulmonary disease, interstitial lung disease, pulmonary vasculitis, acute lung injury, acute respiratory distress syndrome, etc. Many diseases, such as bronchial asthma and sarcoidosis, require long-term hormone therapy. However, glucocorticoids are a class of drugs with very obvious adverse reactions. Long-term application or high-dose application can often lead to a ser...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/661A61K31/573A61K47/48A61K47/42A61P11/00A61P11/06A61P9/00
Inventor 李惠萍陈学远
Owner SHANGHAI PULMONARY HOSPITAL AFFILIATED TO TONGJI UNIV
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