Preparation method for porous monolithic column immobilized enzyme micro-reactor

A technology of immobilized enzymes and microreactors, applied in the direction of supporting/immobilizing microorganisms, enzyme production/bioreactors, biochemical equipment and methods, etc. Long service life, high enzyme activity and low back pressure

Inactive Publication Date: 2012-03-28
GUANGXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability of the immobilized enzyme by physical adsorption is poor, and the enzyme is easy to lose, resulting in a short service life; the chemical bonding method and the cross-linking method often lead to a decrease in enzyme activity or even loss of activity due to relatively severe immobilization chemical reaction conditions

Method used

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  • Preparation method for porous monolithic column immobilized enzyme micro-reactor
  • Preparation method for porous monolithic column immobilized enzyme micro-reactor
  • Preparation method for porous monolithic column immobilized enzyme micro-reactor

Examples

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Embodiment 1

[0022] Example 1: Preparation of porous monolithic column immobilized α-glucosidase microreactor and its application

[0023] refer to figure 1 , the preparation method of porous monolithic column immobilized enzyme microreactor, concrete steps are as follows:

[0024] (1) Synthesis of gold nanoparticles

[0025] Measure 100mL of deionized water and place it in a 250mL round-bottomed flask, heat to reflux with stirring until boiling, then add 1mL of 1% (w / w) chloroauric acid, then continue to heat until the solution boils violently, quickly add 2.5 mL 1% (w / w) sodium citrate, continue to keep the solution boiling for 15min. During this time the color of the solution changed from gray to wine red. Naturally cool to room temperature after removing the heat source, figure 2 It is a transmission electron microscope image of the prepared gold nanoparticles, and it can be seen from the figure that the gold nanoparticles are spherical, and the particle diameter is about 15nm....

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Abstract

The invention discloses a preparation method for a porous monolithic column immobilized enzyme micro-reactor. The porous monolithic column immobilized enzyme micro-reactor is prepared according to the preparation method as follows: firstly, a poly (GMA-co-EDMA) monolithic column is prepared; and then the poly (GMA-co-EDMA) monolithic column is modified by cysteamine and gold nanoparticles so as to obtain a porous polymer monolithic column, wherein, the gold nanoparticles are immobilized on the surface of the porous polymer monolithic column. Enzyme is immobilized to the hole surfaces of the porous monolithic column modified by the gold nanoparticles by utilizing the action of Au-NH2 bond, and then the porous monolithic column immobilized enzyme micro-reactor can be obtained. The preparation method has the advantages as follows: the preparation method is simple; enzyme immobilization reaction conditions are moderate; the permeability of the enzyme micro-reactor is good; large immobilized enzyme quantity can be achieved; the activity, the service life and the stability of enzyme are high in comparison with free solution enzymolysis, and enzyme can be used repeatedly.

Description

technical field [0001] The invention relates to a chemical reactor, in particular to a method for preparing a porous monolithic column-immobilized enzyme microreactor. Background technique [0002] Enzyme-catalyzed reactions are characterized by high efficiency, rapidity, and high selectivity, so they have received extensive attention. Compared with the free enzyme, the immobilized enzyme has the following advantages: (1) the enzyme can be reused, which reduces the cost of use; (2) the stability of the enzyme is greatly improved. The size, permeability, chemical properties and mechanical strength of the support material on which the enzyme is immobilized may all affect the properties of the immobilized enzyme. Therefore, the choice of carrier matrix is ​​an important factor affecting the activity and usability of the prepared bioreactor. In recent years, porous monolithic columns have become a carrier for immobilizing biomacromolecules. Porous monolithic columns were chos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12N11/14C12N11/08
CPCC12M25/16C12M21/18
Inventor 叶芳贵张爱珠陆俊宇赵书林
Owner GUANGXI NORMAL UNIV
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