Complex enzyme preparation for aquatic animal and preparation process thereof
A compound enzyme preparation and aquatic animal technology, applied in animal feed, animal feed, application, etc., can solve the problems of enzyme protein inactivation and activity decline, and achieve the effects of reducing nutrient content, enhancing enzyme production capacity, and high activity
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[0024] The first step: carry out ultraviolet radiation mutagenesis to the Aspergillus oryzae strains producing neutral protease and cellulase and the Aspergillus niger strains producing xylanase and mannanase, select high-temperature-resistant and high-yield strains, and store them. Subculture the preserved strains;
[0025] Step 2: Inoculate the preserved Aspergillus oryzae strains and Aspergillus niger strains on the slant of the wort agar medium respectively, place them in a constant temperature incubator, and incubate at 30°C for 76h-82h, until the slant is covered with hyphae and After the spores, add an appropriate amount of sterile water to wash the spores respectively to make the first-grade seed suspension of Aspergillus oryzae and the first-grade seed suspension of Aspergillus niger;
[0026] The third step: get 70wt% of soybean meal, 26wt% of wheat bran, 3wt% of ammonium sulfate, and 1wt% of potassium dihydrogen phosphate and mix them uniformly to make a solid cultu...
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