Complex enzyme preparation for aquatic animal and preparation process thereof

A compound enzyme preparation and aquatic animal technology, applied in animal feed, animal feed, application, etc., can solve the problems of enzyme protein inactivation and activity decline, and achieve the effects of reducing nutrient content, enhancing enzyme production capacity, and high activity

Inactive Publication Date: 2012-04-04
宝鸡市星星协力生物有限公司
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AI Technical Summary

Problems solved by technology

[0003] Due to the particularity of the digestive system of aquatic animals, the processing of aquatic feed requires shaping and granulation processes, but the high temperature (generally 80°C) during the granulation process will cause most of the enzyme proteins to be inactivated, and the activity will decrease by 50-80%. even ineffective

Method used

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  • Complex enzyme preparation for aquatic animal and preparation process thereof
  • Complex enzyme preparation for aquatic animal and preparation process thereof

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Embodiment Construction

[0024] The first step: carry out ultraviolet radiation mutagenesis to the Aspergillus oryzae strains producing neutral protease and cellulase and the Aspergillus niger strains producing xylanase and mannanase, select high-temperature-resistant and high-yield strains, and store them. Subculture the preserved strains;

[0025] Step 2: Inoculate the preserved Aspergillus oryzae strains and Aspergillus niger strains on the slant of the wort agar medium respectively, place them in a constant temperature incubator, and incubate at 30°C for 76h-82h, until the slant is covered with hyphae and After the spores, add an appropriate amount of sterile water to wash the spores respectively to make the first-grade seed suspension of Aspergillus oryzae and the first-grade seed suspension of Aspergillus niger;

[0026] The third step: get 70wt% of soybean meal, 26wt% of wheat bran, 3wt% of ammonium sulfate, and 1wt% of potassium dihydrogen phosphate and mix them uniformly to make a solid cultu...

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Abstract

The invention relates to a complex enzyme preparation for aquatic animals and a preparation process thereof. The complex enzyme preparation comprises 5,000-7,000u/g of neutral protease activity, 2,000-3,000u/g of mannose activity, 3,000-4,000u/g of xylanase activity and 2,000-3,000u/g of cellulose activity. A mixed fermentation technology of two high-temperature resistant high-yield strains is adopted in the preparation process. The preparation process of the invention is simple and controllable and is easier for operation, and the obtained product has strengthened enzyme producing capability and strengthened high-temperature resistant characteristics of the produced enzyme and can completely tolerate tempering temperature during the production of aquatic feed and maintain higher activity; and the invention can reduce and eliminate chime viscosity improvement and cellulose barrier effect caused by antinutritional factors and supplement the deficiency of endogenous digestive protease, is good for the digestive absorption of high protein aquatic materials, lowers nutrition content in the excrement of the aquatic animals, reduces pollution to a water body, improves water quality and the animal production performance and reduces the bait coefficient and the breeding cost.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a compound enzyme preparation for aquatic animals and a preparation process thereof. Background technique [0002] Fish and shrimp aquatic animals have a higher demand for protein than terrestrial animals, and the protein level is generally required to be between 25% and 50%. Therefore, the protein content of aquatic feed is very high, and the endogenous protease of aquatic animals often cannot make full use of protein. , After adding exogenous protease, it can make up for the deficiency of endogenous protease, increase the protein absorption rate by about 10%, and reduce environmental pollution while saving protein. At the same time, the digestive tract of aquatic animals such as fish and shrimp is quite different from that of other animals. The digestive tract is short, the time for food to stay in the digestive tract is short, and the digestion and absorptio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/165
Inventor 王刚刘丽李冬梅
Owner 宝鸡市星星协力生物有限公司
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