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Preparation method of quasi-microelectrode and application of quasi-microelectrode to simultaneous detection of epinephrine and uric acid

A technology of microelectrode and sodium dodecylbenzene sulfonate, which is applied in the field of electrochemical analysis and detection, can solve the problems of difficult direct detection, small size, difficult detection, etc., and achieve low production cost, fast production process and fast production process. while the simple effect

Inactive Publication Date: 2012-04-04
SHANGHAI UNIV
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Problems solved by technology

[0005] In addition, some actual samples are small in size, which brings certain difficulties to direct detection.
The diameter of the common electrode is generally 3mm, and the diameter of the calomel reference electrode is 3mm. The three-electrode system composed of them and the platinum electrode is difficult to detect the actual sample with a small volume.

Method used

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  • Preparation method of quasi-microelectrode and application of quasi-microelectrode to simultaneous detection of epinephrine and uric acid
  • Preparation method of quasi-microelectrode and application of quasi-microelectrode to simultaneous detection of epinephrine and uric acid
  • Preparation method of quasi-microelectrode and application of quasi-microelectrode to simultaneous detection of epinephrine and uric acid

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Embodiment 1

[0022] The preparation method and steps of the modified quasi-microelectrode in the present embodiment are as follows:

[0023] A Preparation method of quasi-microelectrodes: Mix graphite and paraffin oil in a mortar with a mass ratio of 7:3, stir until uniform, and then put the mixture into a cleaned glass tube with a diameter of 0.5 mm. Insert a copper wire into contact with the mixture at the other end of the glass tube. Grind the graphite end of the quasi-microelectrode on smooth weighing paper and set aside;

[0024] B. Preparation of sodium dodecylbenzenesulfonate / naphthol modified quasi-microelectrode: soak the prepared quasi-microelectrode in a mixed solution of sodium dodecylbenzenesulfonate and naphthol, in which dodecylbenzenesulfonate The concentration of sodium dodecylbenzenesulfonate is 5mM, and the mass concentration of naphthol is 0.05%. After soaking for 6 hours, the sodium dodecylbenzenesulfonate / naphthol solution is adsorbed to the electrode surface by self...

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Abstract

The invention relates to a reparation method of a sodium dodecyl benzene sulfonate / naphthol modified quasi-microelectrode and application of the quasi-microelectrode as an electrochemical sensor to rapid determination of epinephrine and uric acid, and belongs to the technical field of chemical analysis and detection. The invention utilizes catalytic oxidation effect of the electrochemical sensor, prepared from the sodium dodecyl benzene sulfonate / naphthol modified carbon paste quasi-microelectrode, on epinephrine and uric acid to carry out sensitive quantitative analysis on epinephrine and uric acid by a concentration-current curve method. The invention has main points that a working electrode has a small diameter of 0.5mm, and a three-electrode system comprising the quasi-microelectrode, a platinum filament and an Ag / AgCl reference electrode can carry out detection on microscale solution. After drying under a normal temperature, the sodium dodecyl benzene sulfonate / naphthol modified quasi-microelectrode forms a self-assembling modification layer to realize stable catalysis on oxidation of epinephrine and uric acid.

Description

technical field [0001] The invention relates to the preparation of a quasi-microelectrode modified by sodium dodecylbenzenesulfonate and its application as a perelectrochemical sensor for rapid determination of adrenaline and uric acid solutions, belonging to the technical field of electrochemical analysis and detection. Background technique [0002] Epinephrine is an important hormone secreted from the adrenal organ in the body, which acts as a chemical mediator to transmit nerve impulses to other organs. In medicine, epinephrine can be used as a drug to treat diseases such as cardiotonic agents, allergies, and sepsis. If the concentration of adrenaline in the human body changes, it may cause some diseases. [0003] Uric acid is the main product of purine metabolism. If its concentration in the human body changes, it will cause pneumonia, gout and other diseases. Uric acid and epinephrine coexist in the fluid of the human body. If the two important biological substances c...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 丁亚平王碧珺罗立强李丽王玉龙
Owner SHANGHAI UNIV
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