Screening method for IP3 receptor target medicine based on fluorescence polarization analysis

A technology of fluorescence polarization and screening method, which is applied in the field of screening of IP3 receptor target drugs, can solve the problems of high time and cost, and achieve the effect of accurate drug efficacy and accurate prediction
CN102410995BActive Publication Date: 2013-07-31SICHUAN HUIYU PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN HUIYU PHARMA
Publication Date
2013-07-31

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Abstract

The invention discloses a screening method for IP3 receptor target medicine based on fluorescence polarization analysis. The screening method measures the dissociation equilibrium constant KD value of ligands at different temperatures for the first time and initially measures the free energy change of combined ligands at different temperatures according to the dissociation equilibrium constant KDvalue of ligands at different temperatures. Compared with the existing most accurate medicine efficacy forecasting techniques, such as electrophysiology, Nuclear Magnetic Resonance and the like, the screening method provided by the invention can forecast medicine efficacy more accurately since the function of medicine on target can be accurately quantized by thermodynamics. In addition, the screening method has characteristics of high flux and mini-experiment property. Therefore, the screening method provided by the invention will be the trend for forecasting target medicine efficacy.
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Description

technical field

[0001] The present invention relates to the technical field of targeted drug efficacy prediction methods, in particular, to IP based on fluorescence polarization analysis 3 Screening methods for receptor target drugs. Background technique

[0002] In 1926 Perrin first described the phenomenon of fluorescence polarization he observed in a research paper. Fluorescent substances in solution are irradiated with a beam of polarized light of a single wavelength, which absorbs and emits corresponding polarized fluorescence. If the excited fluorescent substance is in a static state, the substance will still maintain the polarization of the original excitation light. If it is in a moving state, the polarized light emitted by the substance will be different from the polarization characteristics of the original excitation light, which is called fluorescence. depolarization phenomenon.

[0003] In recent years, technologies based on this physical phenomenon are playin...

Claims

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