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Chromogenic medium used for detecting esherichia coli O157:H7

A chromogenic medium, O157 technology, applied in the direction of microbe-based methods, microbiological determination/inspection, microbiology, etc., can solve problems such as false positives, and achieve the effects of short detection cycle, strong operability, and high detection sensitivity

Active Publication Date: 2013-09-11
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there are currently found Escherichia coli strains that do not ferment sorbitol and are positive for β-glucuronidase, but O157:H7 serum does not agglutinate, so there are certain false positives
On the other hand, there are also bacteria like Enterobacter sakazakii in Enterobacter, which, like E. coli O157:H7, does not ferment sorbitol and is positive for β-galactosidase, but the chromogenic medium of the above principle cannot Differentiate this type of bacteria from E. coli O157:H7

Method used

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  • Chromogenic medium used for detecting esherichia coli O157:H7
  • Chromogenic medium used for detecting esherichia coli O157:H7
  • Chromogenic medium used for detecting esherichia coli O157:H7

Examples

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Effect test

Embodiment 1

[0032]A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 14g of agar, 10g of peptone, 4g of beef extract powder, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , 5-bromo-4-chloro-3-indole-β-galactoside 0.1g, 5-bromo-6-chloro-3-indole-β-glucuronide 0.08g, isopropyl-β- D-thiogalactopyranoside (IPTG) 0.1g, ox bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

Embodiment 2

[0034] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 12g of agar, 12g of peptone, 3g of beef extract powder, 7g of sodium chloride, 15g of sorbitol, 4g of inositol, and 0.005g of neutral red , 5-bromo-4-chloro-3-indole-β-galactoside 0.05g, 5-bromo-6-chloro-3-indole-β-glucuronide 0.3g, IPTG 0.05g, ox bile Salt 1g, potassium tellurite 3mg, cefixime 0.01mg.

Embodiment 3

[0036] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 20g of peptone, 7g of beef extract powder, 6g of sodium chloride, 20g of sorbitol, 7g of inositol, and 0.03g of neutral red , 5-bromo-4-chloro-3-indole-β-galactoside 0.2g, 5-bromo-6-chloro-3-indole-β-glucuronide 0.05g, IPTG 0.2g, ox bile Salt 5g, potassium tellurite 5mg, cefixime 0.06mg.

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Abstract

The invention discloses a chromogenic medium used for detecting esherichia coli O157:H7. The medium comprises agar, peptone, beef extract powder, sodium chloride, sorbitol, inositol, neutral red, beta-galactosidase chromogenic substrate, beta-glucuronidase chromogenic substrate, isopropyl-beta-D- thiogalactopyranoside, natrium taurocholicum, potassium tellurite and cefixime. The chromogenic medium used for detecting esherichia coli O157:H7 of the present invention has the advantages of high sensitivity, good specificity, direct bacterial strain discrimination according to colony color, short detection period and strong operationality, is suitable for treating high-reflux samples, is capable of comprehensively, systematically and accurately detecting and preliminarily identifying the esherichia coli O157:H7 in food production and environment, and provides a novel approach for rapidly detecting the microbes.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique [0002] Food microbiological safety is the top priority in food safety. Enterohemorrhagic Escherichia coli (EHEC) O157 is a group of Escherichia coli that can cause bloody diarrhea and enteritis in humans, including O157: H7, O157: NM, O26: H11, O111: H8, O125: NM, O121: H19, O45: H2, O4: NM, O145: NM, O5: NM, O91: H21, O103: H2, O113: H2 and other serotype partial strains. Among them, Escherichia coli O157: H7 serotype is the representative strain, which is a serious intestinal infectious pathogen newly discovered in recent years. [0003] Escherichia coli O157:H7 is basically similar to Escherichia coli in other common features except that it does not ferment or slowly ferment sorbitol and the encoded β-glucuronidase is inactive. The traditional detection method of Escheri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/19
Inventor 卢勉飞蔡芷荷刘云林吴清平容艳芬陈佐威
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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