Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing ox gal stone

A technology for ox gallstones and ox bile is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, unknown raw materials, etc. Improve production efficiency and ensure product quality

Active Publication Date: 2012-10-31
WUHAN DAPENG PHARML
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the continuous expansion of production scale, the active ingredients in the original method are easy to decompose, and the cycle of natural drying is too long, which can no longer meet the requirements of larger-scale industrial production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Obtaining ox bile

[0037] Take out the fresh ox bile, adjust the taurine content to 3%, and store it at low temperature for later use.

[0038] (2) Preparation of fermented ox bile

[0039]a. Put the ox bile in the fermenter, turn on the steam to raise the temperature of the liquid in the fermenter to 105°C, sterilize for 15 minutes, and then turn off the steam. Turn on the cooling water to lower the temperature, and when the temperature drops to room temperature, open the inoculation port cover, and after aseptic treatment, inoculate the combined bacterial solution of E. , cap the inoculation port tightly. Turn on stirring, adjust the amount of steam, and stabilize the temperature of the feed liquid to 42°C for fermentation.

[0040] b. After 72 hours of fermentation, start stirring, adjust the amount of steam to raise the temperature to 105°C, maintain the temperature at 105°C, sterilize the fermentation broth for 15 minutes, turn on the cooling water to ...

Embodiment 2

[0051] (1) Obtaining ox bile

[0052] Take out the fresh ox bile, adjust the taurine content to 5%, and store it at low temperature for later use.

[0053] (2) Preparation of fermented ox bile

[0054] a. Put the ox bile in the fermenter, turn on the steam to raise the temperature of the liquid in the fermenter to 108°C, sterilize for 15 minutes, and then turn off the steam. Turn on the cooling water to lower the temperature. When the temperature drops to room temperature, open the inoculation port cover. After aseptic treatment, inoculate Escherichia coli into the ox bile with a ratio of 300 ml bacterial cell solution per 10,000 ml of ox bile, and tightly cover the inoculation port. Turn on stirring, adjust the amount of steam, and stabilize the temperature of the feed liquid to 40°C for fermentation.

[0055] b. After 60 hours of fermentation, start stirring, adjust the amount of steam to raise the temperature to 108°C, maintain the temperature at 108°C, sterilize the ferm...

Embodiment 3

[0066] (1) Obtaining ox bile

[0067] Take out fresh ox bile, adjust the taurine content to 7%, and store it at low temperature for later use.

[0068] (2) Preparation of fermented ox bile

[0069] a. Put the ox bile in the fermenter, turn on the steam to raise the temperature of the liquid in the fermenter to 110°C, sterilize for 15 minutes, and then turn off the steam. Turn on the cooling water to lower the temperature. When the temperature drops to room temperature, open the inoculation port cover. After aseptic treatment, inoculate 500 ml of bacterial solution per 10,000 ml of ox bile, inoculate Enterococcus into ox bile, and tightly cover the inoculation port. Turn on stirring, adjust the amount of steam, and stabilize the temperature of the feed liquid to 37°C for fermentation.

[0070] b. After 48 hours of fermentation, start stirring, adjust the amount of steam to raise the temperature to 110°C, maintain the temperature at 110°C, sterilize the fermentation broth for ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing ox gal stone. The method comprises the steps of: inoculating bacterial liquid to oxgall, fermenting, and then inactivating utilizing steam; adding saturated calcium oxide solution and sodium metabisulfite to the fermented oxgall, stirring, heating to boil, and leaching to obtain a red-brown precipitate; adding bilirubin, cholic acid, deoxycholic acid, zinc sulfate and magnesium sulfate to the precipitate, uniformly stirring and mixing, refrigerating, and vacuum-drying at low temperature; adding fermented oxgall, an antioxidant and purified waterto composite calcium bilirubinate at 15-37 DEG C, sufficiently stirring, and standing to obtain stone-forming gall; adjusting the pH value of the stone-forming gall to below 6.8 with acid liquor, then carrying out off-axial oriented rotation, and stopping rotation until the spheroid stone is formed; and reshaping and freeze-drying the stone to obtain a finished product. The method provided by theinvention has shorter production period and higher efficiency, and is suitable for requirements of larger-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to a method for manufacturing a traditional Chinese medicine raw material, in particular to a method for preparing bovine gallstones. Background technique [0002] Bovine gallstone, namely bezoar, is a precious Chinese herbal medicine. It has the functions of clearing heart, clearing phlegm, resuscitation, cooling liver, calming wind and detoxification. Many famous and precious Chinese patent medicines in our country, such as Angong Niuhuang Pills and Liushen Pills, are all formulated with bezoar as the main medicine. However, the natural stone formation rate in cattle is only 1-2%. Therefore, medicinal resources are very scarce and expensive, and relying on imports not only costs a lot of foreign exchange, but also restricts the development of my country's traditional medicine industry. [0003] Since 1956, Chinese medical workers have been actively engaged in the research of arti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/413
Inventor 石聿新蔡红娇骆小梅
Owner WUHAN DAPENG PHARML
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com