Xanthobacter flavus DT8 and the use thereof for degrading cyclic ethers
A compound, yellow technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of bacteria expansion and cultivation difficulties
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Embodiment 1
[0042] Screening and Identification of Embodiment 1 Flavobacterium DT8 Strains
[0043] (1) Strain screening
[0044]a. Preliminary screening: The activated sludge from the aerobic tank of a sewage station of a pharmaceutical factory in Shanghai is mixed with inorganic salt medium at 1:1 (v / v) to make a mixed medium and put it in a wide-mouth bottle. 1,4-dioxane is used as the substrate as the carbon source and energy source, and the water bath temperature is 30°C for aerated acclimatization culture, and the substrate is added once every 2 days. Substrate, so that the concentration of 1,4-dioxane reaches 500mg / L after adding, and the mixed culture medium is replaced every 7 days. After 7 to 9 months, the domesticated sludge can degrade about 200mg / L of dioxane on average every day, indicating that If the acclimatization is effective, this sample is transferred to a shaker flask for further enrichment;
[0045] b. Re-screening and separation and purification: Inoculate the ac...
Embodiment 2
[0052] The preparation of the cell fermentation liquid containing DT8 bacterium of embodiment 2 yellow yellow bacillus DT8
[0053] 1) Slant culture: inoculate the R2A solid culture medium with Flavobacterium flavum DT8, and culture at 30° C. for 48 hours to obtain slant bacteria.
[0054] 2) Seed culture: pick the slant bacteria prepared in step 1) and inoculate it into the R2A liquid medium, culture at 30°C for 24 hours, and obtain the seed liquid;
[0055] 3) Fermentation culture: Inoculate the seed liquid prepared in step 2) to the fermentation medium with an inoculum size of 10%, in the fermenter at 30°C, with a pH value of 7.0, and maintain dissolved oxygen (DO) at 2.0-3.0, and cultivate for 48 hours to obtain The cell fermentation liquid of DT8 bacteria; the cell fermentation liquid containing DT8 bacteria finally measured the cell OD 600 It reached about 2.0, and the dry bacterial concentration reached 0.77g / L.
Embodiment 3
[0056] The preparation of the cell fermentation broth containing DT8 bacterium of embodiment 3 yellow yellow bacillus DT8
[0057] 1) Incline culture and seed culture methods are the same as in Example 2.
[0058] 2) Fermentation culture: Inoculate the prepared seed liquid into 3.5 L of R2A liquid medium with an inoculum size of 10%, add 0.5 g of glycerol as an antifoaming agent in the fermenter, and maintain the dissolved oxygen (DO) at 30°C with a pH value of 7.0 2.0~3.0, cultivated for 48 hours, and obtained the cell fermentation liquid containing DT8 bacteria; the cell fermentation liquid containing DT8 bacteria was finally measured to measure the cell OD 600 reached 4.0, and the dry cell concentration of the cell fermentation liquid containing DT8 bacteria reached 1.54g / L.
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