Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for modifying micro-fluidic chip by using chiral selective magnetically-functionalized graphene

A microfluidic chip and selective technology, applied in the field of micro-total analysis, can solve the problems of high-efficiency loading of unfavorable biomolecules and maintenance of biological activity, etc., to achieve the effect of improving the reuse rate, simple and controllable operation, and saving modification time

Inactive Publication Date: 2014-05-14
NANCHANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surface of the commonly used chip material PDMS has strong hydrophobicity, which is not conducive to the efficient loading of biomolecules and the maintenance of biological activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for modifying micro-fluidic chip by using chiral selective magnetically-functionalized graphene
  • Method for modifying micro-fluidic chip by using chiral selective magnetically-functionalized graphene
  • Method for modifying micro-fluidic chip by using chiral selective magnetically-functionalized graphene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Fabrication of PDMS chip: Using the GaAs positive mold (produced by the 55th Institute of Electronics in Nanjing) as a template, fabricate a typical cross-shaped PDMS microfluidic chip channel, such as figure 1 shown. The specific production process is as follows: Take a certain amount of PDMS monomer and curing agent according to 10:1 (mass ratio), mix evenly, degas, pour on the GaAs template, and cure at 70 oC for 2 hours. After cooling, peel off the PDMS chip containing the cross-shaped channel from the template, cut it into the desired shape with a blade, and punch holes in the buffer pool, sample pool and sample waste pool with a puncher to form a diameter of 3 mm holes. At the same time, using the smooth glass plate as a template, follow the same steps to prepare a PDMS chip without microchannels as a cover slip. The PDMS chip containing the cross channel and the PDMS cover sheet without the channel were ultrasonically cleaned with secondary water, methanol, and...

Embodiment 2

[0038] (1) Using the improved Hummers method (Hummers W S, Offeman R E. Journal of the American Chemical Society, 1958, 80(6): 1339~1339. Cote L J, Kim F, Huang J. Journal of the American Chemical Society, 2009, 131(3): 1043~1049. Shin H J, Kim K K, Benayad A, et al. Advanced Functional Materials, 2009, 19(12): 1987~1992) Preparation of GO: 0.5 g graphite powder and 0.5 g NaNO 3 Added to 23 mL of 18 mol / L concentrated H 2 SO 4 In ice bath, slowly add 3 g KMnO 4 , after stirring well to make it evenly mixed, transfer the solution to a water bath at 35 ± 5 °C and stir for an hour to form a beige paste. Add 40 mL of water, stir at room temperature for 30 min, then dilute to 140 mL with water. Add 3 mL of 30% H2 dropwise 2 o 2, the solution turned from dark brown to bright yellow. The obtained product was filtered while it was hot, and the product was centrifuged and washed with double distilled water until the supernatant was neutral, and centrifuged at a speed of 12000 r...

Embodiment 3

[0045] GO / Fe 3 o 4 Modification process of / BSA composite on the surface of PDMS microchannel

[0046] (1) The manufacturing process of the simple PDMS microfluidic chip refers to the steps in Example 1.

[0047] (2) GO / Fe 3 o 4 Modification process of / BSA composite material on the surface of PDMS microchannel: First, the PDMS channel was rinsed with double distilled water for 10 min; a permanent magnet was placed on the upper and lower sides of the chip (attracted poles facing each other), and the prepared 2 mg mL -1 GO / Fe 3 o 4 / BSA composite was continuously pumped into the separation pipe for 5 min, and under the action of an external magnetic field, the GO / Fe 3 o 4 The / BSA composite was quickly and controllably immobilized in the PDMS channel, and the modified chip was placed in the refrigerator for 1 hour; the channel was continuously washed with buffer solution for 5 min to wash off the residue in the separation channel, and the GO chip was obtained. / Fe 3 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for modifying a polydimethylsiloxane (PDMS) chip micro-channel based on chiral selective magnetically-functionalized graphene oxide, belonging to the technical field of micro-fluidic chips. A GO / Fe3O4 / BSA modified PDMS chip micro-channel is obtained by using the method. A test result shows that the PDMS chip micro-channel modified by a GO / Fe3O4 / BSA composite material has strong hydrophilic property and high stability, and the efficient separation of model chiral module D / L-tryptophane is successfully realized. The GO / Fe3O4 / BSA composite material prepared by using the method has good magnetic performance, the operations such as controllable assembly and the like of a GO / Fe3O4 / BSA composite nano material in the PDMS chip micro-channel can be realized only under the condition that an external magnetic field is applied, the modification time is greatly saved, and the reutilization ratio of the chip is also increased.

Description

technical field [0001] The invention relates to a micro-total analysis technology, and provides a fast and controllable method for immobilizing GO / Fe on the surface of a PDMS microchip channel. 3 o 4 The invention discloses a method for / BSA composite material, which belongs to the technical field of microfluidic chips. Background technique [0002] At present, the chemical drugs used by humans to treat various diseases are mostly small organic molecules with less than 50 atoms, most of which have one or two chiral centers, and their pharmacological effects are achieved through strict interactions with macromolecules in the body. chiral matching and molecular recognition. A large number of studies have shown that the drug activity of a drug containing a chiral center is closely related to the three-dimensional configuration of the molecule. Often a stereoisomer has medicinal effect, but its mirror image molecule has little medicinal effect, or even has no medicinal effect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B81C1/00B01L3/00
Inventor 梁汝萍孟祥英邱建丁刘春鸣
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com