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Culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and fermentation method thereof

A technology for denitrifying Pseudomonas and a fermentation method, which is applied to the culture medium and fermentation field for the fermentation of Pseudomonas denitrifying to produce vitamin B12, can solve the problem of large fluctuations in product quality, great influence of fermentation units, and restrictions on industrial production, etc. problems, to achieve the effect of reducing comprehensive costs, reducing waste water discharge, and saving the amount of raw and auxiliary materials

Active Publication Date: 2013-12-11
NINGXIA KINGVIT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of artificial planting and harvesting seasons, the product quality of beet molasses fluctuates greatly all the time.
The seasonality of beet molasses production is also particularly strong. The production season of beet molasses is from October to March of the following year. The supply is relatively sufficient, the molasses is fresh and of good quality, and the production of beet molasses in the remaining 6 months The output is obviously insufficient, which seriously restricts the vitamin B 12 industrial production; and the quality of beet molasses varies greatly due to the different origins of beet 12 The fermentation unit has a greater impact; in addition, the storage and transportation costs of molasses are also relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Primary seed medium preparation process: within 1m 3 Add artificial molasses to the primary seed tank (including maltose 2.0kg, thiamine 7g, riboflavin 7g, biotin 7g) 、 Corn steep liquor 14L, magnesium oxide 18g, 5,6-dimethylbenzimidazole 18g, diammonium hydrogen phosphate 40g, zinc sulfate 4g, calcium carbonate 20g, betaine 100g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 31 minutes, cool down and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and wait for inoculation.

[0027] Secondary seed medium preparation process: at 10m 3Add artificial molasses (including maltose 26kg, thiamine 80g, riboflavin 80g, biotin 80g), corn steep liquor 200L, betaine 3kg, magnesium oxide 200g, diammonium hydrogen phosphate 400g, zinc sulfate 50g, Calcium carbonate 800g, 5, 6-dimethylbenzimidazole 700g, urea 40g. Sterilization is carr...

Embodiment 2

[0035] Primary seed medium preparation process: within 1m 3 Add artificial molasses to the primary seed tank (including maltose 2.1kg, thiamine 7.5g, riboflavin 7.5g, biotin 7.5g) 、 Corn steep liquor 15L, magnesium oxide 20g, 5,6-dimethylbenzimidazole 20g, diammonium hydrogen phosphate 42g, zinc sulfate 4.5g, calcium carbonate 25g, betaine 105g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 31 minutes, cool down and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and wait for inoculation.

[0036] Secondary seed medium preparation process: at 10m 3 Add artificial molasses (maltose 26.5kg, thiamine 85g, riboflavin 85g, biotin 85g), corn steep liquor 210L, betaine 3.1kg, magnesium oxide 250g, diammonium hydrogen phosphate 450g, zinc sulfate into the secondary seed tank 55g, calcium carbonate 850g, 5,6-dimethylbenzimidazole 750g, u...

Embodiment 3

[0044] Primary seed medium preparation process: within 1m 3 Add artificial molasses to the primary seed tank (including maltose 2.2kg, thiamine 8g, riboflavin 8g, biotin 8g) 、 Corn steep liquor 16L, magnesium oxide 22g, 5, 6-dimethylbenzimidazole 22g, diammonium hydrogen phosphate 45g, zinc sulfate 5g, calcium carbonate 30g, betaine 110g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 31 minutes, cool down and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and wait for inoculation.

[0045] Secondary seed medium preparation process: at 10m 3 Add artificial molasses (including maltose 27kg, thiamine 90g, riboflavin 90g, biotin 90g), corn steep liquor 220L, betaine 3.2kg, magnesium oxide 300g, diammonium hydrogen phosphate 500g, zinc sulfate 60g into the secondary seed tank , calcium carbonate 900g, 5,6-dimethylbenzimidazole 800g,...

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Abstract

The invention relates to a culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and a fermentation method thereof, and a method for producing vitamin B12 by the same. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all of which contain artificial molasses composed of maltose, thiamine, riboflavin and biotin. By substituting the artificial molasses composed of maltose, thiamine, riboflavin and biotin for beet molasses and optimizing the formula of the culture medium, the invention solves the problem that the fluctuation range of fermentation titer is large because the quality of beet molasses is unstable, and acquires a method for stably and effectively producing vitamin B12. The invention saves the doses of raw and auxiliary materials, lowers the comprehensive cost and reduces the waste water discharge.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a kind of Pseudomonas denitrifica fermented to produce vitamin B 12 Culture medium and fermentation method. Background technique [0002] Vitamin B 12 It is a cobalamin compound, mainly used for the treatment of pernicious anemia, and also used in combination with folic acid for the treatment of various megaloblastic anemia, anemia and steatorrhea caused by antifolate drugs. It is also used for the treatment of nervous system diseases (such as neuritis, nerve atrophy). [0003] Currently commercialized vitamin B at home and abroad 12 Almost all are produced by microbial fermentation. Among them, vitamin B is produced by fermentation of Pseudomonas denitrification bacteria 12 The raw and auxiliary materials for production are mainly made of carbon and nitrogen sources such as beet molasses and sucrose and a small amount of inorganic salts for liquid submerged fermentatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/42C12R1/38
Inventor 任勇冷晓红王友善奇乃董媛
Owner NINGXIA KINGVIT PHARMA
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