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High protein feed prepared by using waste yeast mud and its method

A technology for high-protein feed and waste yeast mud is applied in the field of product waste recycling, which can solve the problems of poor drying effect, long drying time, and difficulty in crushing, and achieves improved leached protein content, high protein content, and high drying efficiency. Effect

Inactive Publication Date: 2012-06-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] On the other hand, the yeast sludge after autolysis has a high water content and high viscosity. The current method is mainly high-temperature drying. The dried yeast is easy to agglomerate, hard in texture, and not easy to crush
The value of industrial waste yeast is low. If other drying methods are used for drying, there will be disadvantages such as high energy consumption, long drying time, and poor drying effect.

Method used

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  • High protein feed prepared by using waste yeast mud and its method
  • High protein feed prepared by using waste yeast mud and its method
  • High protein feed prepared by using waste yeast mud and its method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Effect of different water additions, autolysis temperature, and autolysis time on the cell wall breaking rate of waste yeast sludge autolysis

[0029] Take the waste yeast producing β-mannanase for feeding, add appropriate amount of distilled water, mix well, and autolyze at a certain temperature for a period of time; after taking it out, dilute it to a certain multiple, observe it with a hemocytometer under a microscope, and calculate the individual yeast cells Count and calculate the cell wall breaking rate.

[0030] Wherein, cell wall breaking rate (%)=number of yeast cells after autolysis / number of yeast cells before autolysis×100%; the experimental data are the average values ​​of three parallel experiments for each group of samples.

[0031] Design different water addition, autolysis temperature, and autolysis time, and find the optimal condition range for waste yeast sludge autolysis through single factor test. The test design and test results are shown...

Embodiment 2

[0039] Embodiment 2 Response surface analysis method establishes optimum autolysis condition

[0040] Based on the test results obtained in Example 1, a response surface test was designed. According to the Box-Benhnken central combination experiment design principle, select the factor interval that has a greater influence on the autolysis of waste yeast slime. The autolysis time, autolysis temperature and water addition are represented by A, B and C respectively. Each independent variable is represented by The three levels of low, medium and high are coded with -1, 0 and 1 respectively. The three levels selected for autolysis time (A) are: 18h, 24h and 30h; the three levels selected for autolysis temperature (B) are 45°C, 50°C and 55°C; the three levels selected for the water addition (C) are 0%, 50% and 100%, see Table 4.

[0041] Table 4 Experimental factor levels and coding

[0042]

[0043] After the design is completed, carry out the test grouping, use BBX software t...

Embodiment 3

[0050] Example 3 Verification experiment of Kjeldahl method on optimal autolysis conditions of waste yeast sludge

[0051] Take the waste yeast sludge left after fermenting β-mannanase for feeding, put it in a 50ml centrifuge tube, add 50% distilled water, shake evenly, put it at 50°C for 24 hours; take it out and digest it, and use The automatic Kjeldahl azotometer measures the protein content in the supernatant, and the test result is: the mass fraction of the dissolved protein accounting for the dry matter of the yeast slime is 12.3%, which is close to the theoretical value 13.7% obtained in Example 2, thus proving The optimization results are real and effective.

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Abstract

The invention discloses a high protein feed prepared by using waste yeast mud and its method. The method comprises the following steps: adding the waste yeast mud in water and uniformly mixing, self-dissolving for 6-36 hours at the temperature of 30-60 DEG C, adding soybean meal and uniformly stirring, drying to prepare the high protein feed, wherein the waste yeast mud is the waste yeast mud capable of producing beta-mannanase or beer. By calculating every kilogram of waste yeast mud, the amount of the water is 0-2kg, the addition of soybean meal is 0.5-2kg. According to the invention, the high protein feed contains dissolved protein with high concentration, and is safe and nontoxic. An autolysis method is used for breaking wall of the yeast cells, no other substance can be introduced, the operation is simple and the energy consumption is low; soybean meal which is taken as a raising agent is added when drying for effectively raising the drying efficiency, the cost can be saved, the soybean meal is a high protein substance, the obtained product can be taken as the high protein feed without separation, and can be prepared to high protein feed by adding common auxiliary materials.

Description

technical field [0001] The invention relates to the technical field of product waste recycling, in particular to a high-protein feed prepared by using waste yeast mud and a method thereof. Background technique [0002] Yeast is a single-cell eukaryotic microorganism. In addition to being used for fermentation, yeast is also rich in nutrients. The protein content of yeast accounts for more than 50% of its dry weight. Over the past 100 years, the yeast industry has formed a strong production system, and its proportion in the food industry is increasing day by day. However, with the increase of yeast fermentation time and frequency, the activity of yeast will gradually decrease, and even begin to die. Therefore, a large amount of "waste yeast" will be produced in the fermentation industry, mainly including beer yeast and some enzyme-producing yeast. A spent yeast sludge forms at the bottom of the tank. The fermentation function of these "waste yeasts" has been weakened, but t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/06A23K1/14A23K1/175A23K10/38
CPCY02P60/87
Inventor 何国庆高世阳阮晖陈启和
Owner ZHEJIANG UNIV
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