Konjac glucomannan microballoon with uniform dimension and preparation method thereof

A konjac glucomannan, uniform technology, applied in the field of konjac glucomannan microspheres and its preparation, can solve the problems of increased production costs, waste of raw materials, labor and material resources, and achieve uniform size and controllable particle size Effect

Active Publication Date: 2012-06-13
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening process will not only cause waste of raw materials, but also consume a lot of manpower and material resources, result

Method used

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  • Konjac glucomannan microballoon with uniform dimension and preparation method thereof
  • Konjac glucomannan microballoon with uniform dimension and preparation method thereof
  • Konjac glucomannan microballoon with uniform dimension and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of Aqueous Solution of Konjac Glucomannan

[0042] In a 500ml reactor, mix 20ml of 0.5M HCl and 120g of deionized water evenly, pour 20.0g of konjac glucomannan into the mixture, stir evenly, and swell for 5 minutes to form jelly. Cover the reactor with parafilm or a watch glass, put it in an autoclave, and degrade it at 115°C for 30 minutes. After the degradation is complete, take out the reactor and place it at room temperature. Add 40g of 40% NaOH solution, stir to dissolve the slurry, and filter impurities to obtain a clear aqueous solution of 10% konjac glucomannan. The same method can prepare konjac glucomannan aqueous solution with solid content of 0.1%, 4%, 15%, 20%, 40%, etc. for future use.

Embodiment 2

[0044] The hydrophobic membrane with a pore size of 50.2 μm is immersed in the lipophilic substance to fully wet the porous membrane to ensure that the hydrophobic chains on the membrane are completely stretched. 50 ml of xylene containing 0.8 g of Span 800 and 0.4 g of ethyl cellulose was added to a 200 ml reactor, and the temperature was raised to 60°C. Take 20 ml of konjac glucomannan solution with a solid content of 40%, add it to a membrane emulsification device, and press it into the oil phase through a hydrophobic membrane with uniform pore size under constant pressure to obtain a W / O emulsion. After the emulsification is complete, take out the membrane emulsification device. Slowly add 8ml of cross-linking agent epichlorohydrin into the emulsion dropwise for about 0.5h. After the dropwise addition, the temperature was raised to 80° C. and reacted for 8 hours under stirring conditions, and the microspheres were solidified. After repeated washing and filtering, konjac ...

Embodiment 3

[0046] The hydrophobic membrane with a pore size of 19.6 μm is immersed in the lipophilic substance to fully wet the porous membrane to ensure that the hydrophobic chains on the membrane are completely stretched. Add 200ml of liquid paraffin containing 2.0g of Span 60 and 0.5g of lithium ester into a 500ml reactor, and heat up to 40°C. Take 50 ml of konjac glucomannan solution with a solid content of 10%, add it to a membrane emulsification device, and press it into the oil phase through a hydrophobic membrane with uniform pore size under constant pressure to obtain a W / O emulsion. After the emulsification is complete, take out the membrane emulsification device. Slowly add 25ml of cross-linking agent epichlorohydrin into the emulsion for about 1 hour. After the dropwise addition, the temperature was raised to 70° C. and reacted for 8 hours under stirring condition, and the microspheres solidified. After repeated washing and filtering, konjac glucomannan microspheres with a ...

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Abstract

The invention relates to konjac glucomannan microballoons with uniform dimension and a preparation method thereof. The konjac glucomannan microballoons are of a gel type or a macroporous type and have uniform dimension and average particle size in a controllable scope of 10-200 mum. The preparation method comprises the following steps: first degrading konjac glucomannan to obtain a konjac glucomannan aqueous solution as a water phase (W); using a water-insoluble solution dissolved with oil soluble emulsifier as an oil phase (O); pressing the water phase into the oil phase to obtain a W / O type emulsion; adding a cross-linking agent into the emulsion dropwise and stirring for reaction; forming konjac glucomannan into microballoons through crosslinking, washing and collecting to obtain the konjac glucomannan microballoons with uniform dimension. The konjac glucomannan microballoons have uniform dimension and controllable particle size; microballoons do not gather, so that a gathering phenomenon will not occur during preservation; and the konjac glucomannan microballoons can satisfy multiple demands on separation and purification, cell culture microcarrier and diagnosis preparation, etc.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to konjac glucomannan microspheres with uniform size and a preparation method thereof. Background technique [0002] Konjac glucomannan (KGM) is a high-molecular polysaccharide, which is composed of glucose and mannose linked by β-1,4-glycosidic bonds. Dextran has been widely used as a natural polysaccharide material. Konjac glucomannan is very similar to dextran in structure, and has many characteristics of dextran as a biological functional material: high hydrophilicity, containing more activated hydroxyl groups , no non-specific adsorption with biomacromolecules, etc., so the prepared konjac glucomannan microspheres can be used as chromatography media, microcarriers, drug sustained and controlled release carriers and other products. Since the glucomannan molecule contains more active hydroxyl groups, it can be functionalized under certain conditions to introduce...

Claims

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Application Information

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IPC IPC(8): C08L5/02C08J3/24C08J3/16C08J9/26C08J9/28C08B37/02
Inventor 马光辉周炜清苏志国王佳兴熊志冬林丰凯
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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