Deoxyribozyme hydrogel based copper ion detection sensor

A deoxyribozyme water and copper ion detection technology is applied in the field of copper ion detection to achieve the effect of easy operation

Inactive Publication Date: 2012-06-20
INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, complex and expensive instruments such as atomic absorption or ICP-MS are used for the detection of copper ions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation method of copper ion sensor based on deoxyribozyme-hydrogel

[0034]1. Synthesis of acrylic acid phosphoramidite monomer

[0035] The synthesis of acrylate phosphoramidite monomer is divided into three steps: 1) Add 6-amino-1-hexanol (1g, 8,53mmol), triethylamine (2.36mL, 17mmol) into 100mL dichloromethane and ice bath , under anhydrous and oxygen-free conditions, methacryloyl chloride (2.67 g, 2.55 mmol) was added dropwise, and then the whole system was stirred and reacted at room temperature for 2 hours. 2) After the solvent was spin-dried, 10 mL of ethanol and 15% NaOH (4 mL) were added to the product to selectively hydrolyze the product into 6-isocyanatohexyl-methacryloyl chloride. The next reaction was performed after separating 6-isocyanatohexyl-methacryloyl chloride with ethyl acetate on a silica gel column. 3) Dissolve the purified 6-isocyanatohexyl-methacryloyl chloride (0.50g, 2.70mmol) in 10mL of anhydrous dichloromethane, and add N,...

Embodiment 2

[0044] Example 2: Detection of Copper Ions in Water Samples Using Deoxyribozyme Hydrogel Biosensor

[0045] Add 100 μL of the water sample to be tested to the buffer layer on the upper part of the DNAzyme hydrogel biosensor with a pipette, place it in a dry bath at 37°C, and gently suck the buffer layer with a pipette to make the buffer and the added to-be-treated Mix the sample water evenly. Place it in a dry bath at 37°C for 1.5 hours, and observe with the naked eye whether the wine-red hydrogel in the lower layer of the system spreads gradually. If the wine red hydrogel in the lower layer diffuses evenly into the buffer system in the upper layer, the water sample contains copper ions. If the upper and lower wine red hydrogels in the DNAzyme hydrogel visual detection sensor do not diffuse, the water sample does not contain copper ions.

Embodiment 3

[0046] Example 3: Semi-quantitative detection of copper ion concentration in water samples using DNAzyme hydrogel biosensor

[0047] At the same time, add 100 μL of the water sample to be tested and 100 μL of the standard copper ion aqueous solution (concentration requirement is less than 1000 μM) to the buffer layer on the upper part of the two DNAzyme hydrogel biosensors with a pipette, and place them in a dry bath at 37 °C , Use a pipette to gently pipette the buffer layer to make the buffer evenly mixed with the added water sample to be tested. Place it in a dry bath at 37°C for 1.5 hours, and observe the diffusion degree of the wine red hydrogel in the lower layer of the system with the naked eye. If the hydrogel in the sensor added to the water sample to be tested has a large degree of diffusion and disintegration, the The content of copper ions is higher than that of the standard copper ion aqueous solution, otherwise the copper ion content is lower than that of the s...

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PUM

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Abstract

The invention discloses a deoxyribozyme hydrogel based copper ion detection sensor, which is characterized by including deoxyribozyme hydrogel, wherein the deoxyribozyme hydrogel takes the shape of a three-dimensional network which is formed by substrate chains modified by a methacrylic group and the deoxyribozyme hydrogel modified by the methacrylic group through base complementation, pairing and crosslinking; and aurum nanoparticles are coated on the hydrogel. Meanwhile, the invention further discloses a preparation method for the deoxyribozyme hydrogel in the deoxyribozyme hydrogel based copper ion detection sensor, as well as a method for qualitative and semiquantitative detection of the copper ion content in a water sample through the sensor. The invention can realize fast detection of copper ion, requires no expensive complex instruments and devices, has the advantages of high selectivity and high detection speed, and is extremely suitable for onsite detection and laboratory batch sample coarse screening.

Description

technical field [0001] The invention relates to the field of copper ion detection, in particular to the use of DNAzyme hydrogel in copper ion detection sensors. Background technique [0002] Copper is the earliest metal used by humans. Copper ions widely exist in nature. Copper ions are also essential trace elements for humans. However, excessive copper is also very harmful to the human body, mainly caused by heavy metal ions copper. When a large amount of heavy metals remain in the human body, it is easy to cause a burden on the organs in the body, especially the liver and gallbladder. When these two organs have problems, the metabolism in the body will be disturbed, liver cirrhosis, liver Ascites is even more serious. The U.S. Environmental Protection Agency requires that the detection concentration of copper ions in drinking water be no higher than 20 μM. Traditionally, complex and expensive instruments such as atomic absorption or ICP-MS are used for the detection of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 徐敦明吴敏杨朝勇周昱张志刚方恩华
Owner INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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