Method for extracting chitin and antibacterial peptide from fly larvae

An antimicrobial peptide and chitin technology, applied in the field of natural product extraction, can solve problems such as demanding process conditions, high extraction costs, and poor environmental conditions, and achieve the effects of reasonable technical route, friendly production environment, and high economic value

Inactive Publication Date: 2012-07-11
QINGDAO ZHONGREN PHARMA
View PDF1 Cites 32 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the mature process of extracting natural chitin and antimicrobial peptides using fly maggots as raw materials and using comprehensive physical and chemical processes. The existing ex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting chitin and antibacterial peptide from fly larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Get feces to cultivate fly maggot 100g, use 1 / 5000KMnO 4 After rinsing the solution for 10 minutes, put 1 / 100 soybean meal pulp into it and cultivate it for 1 day, so that the fly maggots can drain the feces, and then wash once with clear water to obtain 91g of fresh fly maggots, which are squeezed by a roller to obtain 35g of fly maggot skin and maggot skin. Visceral pulp 56g, chitin and chitosan can be extracted from fly maggot skin, and antibacterial peptide can be extracted from fly maggot visceral pulp.

Embodiment 2

[0019] (1) Preparation of chitin: Add 35g of fly maggot skins to a 250ml three-neck flask, add 50ml of 1% hydrochloric acid solution, heat in a water bath at 38-40°C for 4 hours, filter, wash several times until neutral, dry at 50°C, and dry Add 50ml of 4% NaOH to the maggot skin, heat in a 96-98 water bath for 5 hours, filter, wash several times until neutral, and dry below 50°C to obtain 4.9g of chitin with a yield of 14%.

[0020] (2) Chitosan preparation: Soak chitin 4.9g in 100ml45%-55% NaOH, after continuing to react for 8 hours in a water bath at 98-99°C, prepare 100ml45%-55% NaOH again, Continue to heat in the water bath at 98-99°C for 8-16 hours. When the product is completely dissolved in 1%-2% acetic acid, the reaction is completed, and washed with water until neutral to obtain 3.4g chitosan, the yield is 70% of chitin. %.

[0021] (3) Preparation of chitooligosaccharides: Dissolve 3.4 g of fly maggot chitosan in 100 ml of acetic acid, adjust the pH to 5.5-6, add 4...

Embodiment 3

[0022] Example 3: Take 56g of fly maggot viscera mass slurry and add acetic acid to a concentration of 0.5mol / L, homogenize the slurry, overnight at 4°C, centrifuge, filter to obtain 200ml fly maggot antibacterial peptide acid extract, add alginic acid, and stir for 1 hour , so that the antimicrobial peptide is fully adsorbed by alginic acid, let it stand at 4°C overnight, and filter it with a Buchner funnel to collect the alginic acid that has adsorbed the antimicrobial peptide, elute it with 0.2mol / L hydrochloric acid, collect the eluate, and wash it with solid acetic acid After adjusting the pH value to 3.5 with sodium, salt it out with 320 g / L NaCl. After overnight, dialyze through a dialysis bag, desalt, and dry to obtain 0.6 g of crude antimicrobial peptide.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of product extraction technology and relates to a method for extracting chitin and antibacterial peptide from fly larvae. The method comprises the following steps of: firstly disinfecting and rinsing fresh fly larvae to be clean, then extruding the cleaned fly larvae to separate the fly larvae skin from visceral mass serous fluid; firstly soaking with hydrochloric acid solution, drying after washing, and then soaking with NaOH solution to remove protein; retaining residual liquid, drying after cleaning, adding the NaOH solution after crushing, carrying out water bath at the temperature of 98 DEG C, and heating for reaction; putting chitosan into acetic acid solution, stopping reaction after the chitosan is dissolved, filtering, cleaning, drying at the temperature of 50 DEG C, and obtaining the chitosan; and then adding acetic acid into the visceral mass serous fluid of the fly larvae, homogenizing, standing over the night at the temperature of 4 DEG C, filtering, adsorbing the filtrate with alginic acid, filtering, then eluting the antibacterial peptide adsorbed on the alginic acid with the hydrochloric acid solution, adjusting the pH value of the eluent to be 3.5, carrying out precipitation with NaCl, carrying out dialysis and desalting, drying and obtaining a crude antibacterial peptide product. The method has the advantages of reasonable technical route, low cost, high yield, large economic value, environmentally-friendly effect and effective utilization of the fly larvae resources for preparing natural product varieties.

Description

Technical field: [0001] The invention belongs to the technical field of natural product extraction, and relates to a technical process for comprehensive processing and utilization of fly maggots, in particular to a method for extracting chitin and antibacterial peptides from fly maggots. Background technique: [0002] The reproductive ability of flies ranks first in the insect world. According to literature records, a pair of flies can reproduce 266 billion maggots within 4 months. Feces, sludge from sewage treatment plants, waste from slaughterhouses and waste blood can all act as nutrient sources for fly maggots. Therefore, among similar raw materials, the cost of breeding fly maggots is relatively low. According to literature records, crude fly maggots in dry fly maggots Protein accounts for 55-65%, and the proportion of essential amino acids exceeds that of non-essential amino acids. It is a high-quality protein source. The content of chitin in dried fly maggots accounts...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/08C12S3/02C07K1/30C07K1/34
Inventor 徐权汉夏伟张浩郑德志
Owner QINGDAO ZHONGREN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap