Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties

A technology of Fusarium oxysporum and root rot is applied in the application field of cultivating tomato neck rot and root rot disease-resistant varieties, which can solve the problem that research on tomato neck rot root rot is still blank and other problems, and achieve the effect of promoting development.

Active Publication Date: 2013-04-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Successful isolation, purification and identification of the pathogen of tomato neck rot and root rot in my country, and understanding the growth conditions of the pathogen is a necessary condition for disease-resistant breeding, but research on tomato neck rot and root rot in my country is still blank

Method used

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  • Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties
  • Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties
  • Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, the acquisition and identification of bacterial strain

[0027] 1. Acquisition of strains

[0028] In August 2008, typical diseased plants were collected in the greenhouse of the National Vegetable Engineering Technology Research Center farm. According to the conventional method, the pathogen was isolated and cultured on a PDA plate at 25°C, and single spores were picked and purified. A strain of pathogenic bacteria was isolated and named strain Forl-c.

[0029] 2. Identification of strains

[0030] 1. Morphological identification

[0031] The strain Forl-c was inoculated on a PDA plate, cultured in the dark at 25°C, and the colony morphology was observed. Culture on CLA medium (2% water agar, 20g agar, 1L water, carnation leaf pieces), observe macroconidia, microconidia and chlamydospores, and directly observe microconidia under a 100X inverted microscope How spores are produced.

[0032] For morphological identification, see figure 1 , A is colony ...

Embodiment 2

[0038] The pathogenicity assay of embodiment 2, bacterial strain Forl-c

[0039] Tomato (Lycopersicon esculentum Mill.) inbred line Q-167: National Vegetable Engineering Technology Research Center).

[0040] 1. After culturing the bacterial strain Forl-c on a PDA plate (pH6.5) for 7 days, use distilled water to make a concentration of 10 7 per mL of spore suspension.

[0041] 2. Soak the seeds of the tomato inbred line Q-167 in warm water at 52°C for 30 minutes, and then rinse with clean water.

[0042] 3. Inoculation by root soaking method

[0043] Sow the seeds of step 2 after germination in a nutrient bowl filled with sterilized vermiculite, gently pull up the seedlings when they have 1 true leaf, rinse the root system with water, put the spore suspension obtained in step 1 (set distilled water as contrast) ) for 10 minutes, and then transplanted into a seedling pot filled with sterilized vermiculite and peat (1 mass part of vermiculite+2 mass parts of peat). The plants...

Embodiment 3

[0046] Embodiment 3, the determination of the pathogenic range of bacterial strain Forl-c

[0047] The test plants are as follows:

[0048] Tomato (Lycopersicon esculentum Mill.) inbred line Q-167: National Vegetable Engineering Technology Research Center);

[0049] Cucumber (Citrullus sativus L.) variety 102: Beijing Jingyan Yinong Science and Technology Development Center;

[0050] Beet (Beta vulgaris L.) variety purple leaf beet: Beijing Jingyan Yinong Science and Technology Development Center;

[0051] Kidney bean (Phaseolus vulgaris L.): Beijing Juhong Seedling Technology Co., Ltd.

[0052] Each test plant was carried out in the following experiments:

[0053] 1. After culturing the bacterial strain Forl-c on a PDA plate (pH6.5) for 7 days, use distilled water to make a concentration of 10 7 per mL of spore suspension.

[0054] 2. Soak the seeds of the tested plants in warm water at 52°C for 30 minutes, and then rinse them with clean water.

[0055] 3. Inoculation b...

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Abstract

The invention discloses a fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties. Preservation number of a bacterial strain (bacterial strain Forl-c for short) is China general microbiological culture collection center number 5517 (CGMCC No. 5517). The fusarium oxysporum tomato neck rot root rot specialization type and the application of the fusarium oxysporum tomato neck rot root rot specialization type in cultivation of tomato neck rot rood rot disease-resistant varieties facilitate analysis of pathogenesis of tomato neck rot root rot diseases by tomato neck rot root rot research workers in our country, facilitate research and development of tomato neck rot root rot biocontrol agents,facilitate research of inheritance of resistance and related gene molecular markers of the tomato neck rot root rot diseases, facilitate excavation of disease-resistant genes of the tomato neck rot root rot diseases, facilitate screening and identification of disease-resistant plant materials of the tomato neck rot root rot diseases and other work, lay the foundation for integrated control of the tomato neck rot root rot diseases, have great value for effectively controlling damages of the tomato neck rot root rot diseases, and promote development of disease-resistant breeding of the tomato neck rot root rot diseases.

Description

technical field [0001] The invention relates to a specialized type of Fusarium oxysporum tomato neck rot and root rot and its application in cultivating varieties resistant to tomato neck rot and root rot. Background technique [0002] Tomato neck rot and root rot were first discovered in Japan, and then occurred in many countries and became an important disease affecting greenhouse tomato production. The main symptoms of tomato neck rot and root rot are: browning and rot at the base of the tomato stem and root, and the affected plants have obvious dark brown lesions at the junction of the soil and the base of the stem (around the base of the stem); early infected plants, In the seedling stage of 3-5 leaves, the diseased part is folded and wilted and died; the infected plants in the late stage are infected from the 5-leaf stage to the flowering and fruiting stage, showing that the stem base is constricted, dark brown, and the plants are still upright. Wilting is fatal, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12Q1/02A01N63/04A01P3/00A01C1/00A01G31/00A01G7/00C12R1/77
Inventor 耿丽华柴敏李常保迟胜起王丽君
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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