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Method for preparing allograft acellular dermal matrixes

A decellularized dermis and patch technology, which is applied in the field of preparing acellular dermal matrix medical tissue patches, can solve the problems of matrix damage, complicated operation, and low efficiency, and achieve the effect of short degreasing time, short time and high efficiency

Active Publication Date: 2012-07-18
江苏优创生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing acellular dermal matrix production process has the following disadvantages: the operation is more complicated, the cycle is longer, and the efficiency is lower; it is easy to cause damage to the matrix

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing an acellular dermal matrix medical tissue patch, the tissue patch being prepared from pig skin, cowhide or sheep skin, comprising the following steps:

[0016] a. Process for preparing split skin patches: use a skin machine to make animal skin into split skin sheets with a thickness of 0.1mm, and use a punch to make split skin sheets into different shapes of split skin sheets;

[0017] b. Virus inactivation process: inactivate the tomographic skin patch with a virus inactivation solution composed of 0.1% by mass concentration of peracetic acid and 1.0% by mass concentration of sodium chloride for 30 minutes. The quality of the skin patch and the virus inactivation solution The ratio is 1: 4;

[0018] c. Degreasing process: Use a mixed degreasing agent composed of 1.0% soda ash by mass concentration, 1.0% caustic soda by mass concentration and 0.1% by mass concentration to degreasing for 30 minutes. The mass ratio of the skin sheet and the mixed degreasin...

Embodiment 2

[0023] A method for preparing an acellular dermal matrix medical tissue patch, the tissue patch being prepared from pig skin, cowhide or sheep skin, comprising the following steps:

[0024] a. Process for preparing split skin patches: use a skin machine to make animal skin into split skin sheets with a thickness of 0.1mm, and use a punch to make split skin sheets into different shapes of split skin sheets;

[0025] b. Virus inactivation process: inactivate the tomographic skin patch with a virus inactivation solution composed of 0.1% by mass concentration of peracetic acid and 1.0% by mass concentration of sodium chloride for 30 minutes. The quality of the skin patch and the virus inactivation solution The ratio is 1: 4;

[0026] c. Degreasing process: Use a mixed degreasing agent composed of 1.0% soda ash by mass concentration, 1.0% caustic soda by mass concentration and 0.1% by mass concentration to degreasing for 30 minutes. The mass ratio of the skin sheet and the mixed degreasin...

Embodiment 3

[0031] A method for preparing an acellular dermal matrix medical tissue patch, the tissue patch being prepared from pig skin, cowhide or sheep skin, comprising the following steps:

[0032] a. Process for preparing split skin patches: use a peeling machine to make animal skin into split skin sheets with a thickness of 1.0mm, and use a punch to make split skin sheets into different shapes of split skin sheets;

[0033] b. Virus inactivation process: use the virus inactivation solution composed of 1.0% by mass concentration of peracetic acid and 3.0% by mass concentration of sodium chloride for inactivation of the tomographic skin for 45 minutes. The quality of the skin and the virus inactivation solution The ratio is 1:5.5;

[0034] c. Degreasing process: Adopt a mixed degreasing agent composed of 2.5% soda ash, 2.5% caustic soda and 0.5% sodium lauryl aminopropionate to degrease for 45 minutes. The mass ratio of the skin piece to the mixed degreasing agent is 1:5.5;

[0035] d. Decel...

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PUM

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Abstract

The invention disclose a method for preparing allograft acellular dermal matrixes, comprising the following step of processing animal skins into the allograft acellular dermal matrixes by using processes of preparation of segmented skin, viral inactivation, degreasing, decellularization, freeze-drying, cutting, molding, packaging and irradiation disinfection. The method for the preparing allograft acellular dermal matrixes has the advantages of short degreasing time, high efficiency, complete decellularization and small damage to natural structures of dermal extracellular matrixes.

Description

Technical field [0001] The invention relates to a method for preparing acellular dermal matrix medical tissue patch. Background technique [0002] Acellular dermal matrix medical tissue patch is a tissue engineering project developed on the basis of tissue engineering. The purpose is to use the extracellular matrix of the dermis for tissue repair. It is taken from natural skin tissues and undergoes special physical and chemical treatment to remove the components that may cause immune rejection after implantation in the human body, while retaining the three-dimensional scaffold structure of the original tissue, so it has the effect of inducing tissue regeneration . The existing acellular dermal matrix production process has the following shortcomings: the operation is more complicated, the cycle is longer, and the efficiency is low; it is easy to cause damage to the matrix. Therefore, it is necessary to provide a new technical solution to solve the above-mentioned problems. Sum...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/60
Inventor 潘银根潘华倩
Owner 江苏优创生物医学科技有限公司
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