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Quantum dot/polymer composite nitric oxide fluorescent probe and preparation method thereof

A technology of nitric oxide and fluorescent probes, which is applied in the field of analysis and detection, can solve the problems that have not yet been reported on the research of composite nitric oxide fluorescent probes, and cannot solve the problems of quantum dot dispersion, poorness and agglomeration, and achieve stable fluorescence Long time, easy to popularize and apply, and quickly detect the effect

Active Publication Date: 2012-07-18
GUANGDONG NANHAI ETETB TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common method of compounding quantum dots and polymers is to directly mix pre-prepared quantum dots and polymers, such as Chinese patent CN101759946A, but this method still cannot solve the problems of poor dispersion and agglomeration of quantum dots in the matrix.
So far, there have been no research reports based on quantum dot / polymer composite nitric oxide fluorescent probes

Method used

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  • Quantum dot/polymer composite nitric oxide fluorescent probe and preparation method thereof
  • Quantum dot/polymer composite nitric oxide fluorescent probe and preparation method thereof
  • Quantum dot/polymer composite nitric oxide fluorescent probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Weigh 0.1387g of cadmium acetate (C 4 h 6 CdO 4 2H 2 O) 0.00052 and 0.038g of azobisisobutyronitrile were dissolved in a three-necked flask filled with 8ml of methyl methacrylate and 1ml of methacrylic acid, stirred under nitrogen protection, and heated to 70~90°C for prepolymerization , observe the viscosity of the system during the prepolymerization process, and stop heating immediately when the viscosity of the prepolymer is close to the viscosity of glycerin;

[0033] Weigh 0.15g of sodium sulfide (Na 2 S·9H 2 O) Dissolve in 1.5mL of deionized water, add it dropwise to a three-necked flask, and simultaneously with vigorous stirring, the mixture quickly turns bright yellow;

[0034] Transfer the mixture to a one-necked flask, put it in an oven, and polymerize at 60°C for 20 hours to obtain a CdS / PMMA composite nitric oxide fluorescent probe, see figure 1 . The CdS / PMMA composite NO fluorescent probe is shown in figure 2 . Depend on figure 2 It can be obta...

Embodiment 2

[0039] Example 2 is the same as Example 1, except that in step (2) of Example 1, "weigh 0.15g of sodium sulfide and dissolve it in 1.5mL of deionized water" is replaced by "weigh 0.164g Na 2 SeO 3 ·5H 2 O, 0.8 g NaOH and 3 mL N 2 h 4 ·H 2 O dissolved in 5mL deionized water"; the "polymerization at 60°C for 20h" in step (3) of Example 1 was replaced with "polymerization at 50°C for 12h" to obtain a CdSe / PMMA composite nitric oxide fluorescent probe. It was tested by TEM : CdSe is evenly distributed in the matrix material PMMA, and the spherical nanoparticles with a size of CdSe between about 5nm.

Embodiment 3

[0041] Example 3 is the same as Example 1, except that in step (2) of Example 1, "weigh 0.15 g of sodium sulfide and dissolve it in 1.5 mL of deionized water" is replaced by "5 mL of freshly prepared sodium hydride telluride (NaHTe) solution ", replace the "polymerization at 60°C for 20h" in step (3) of Example 1 with "polymerization at 70°C for 15h" to obtain a CdTe / PMMA composite nitric oxide fluorescent probe. The TEM test shows that: CdTe is evenly distributed in the matrix material PMMA, and the CdTe is spherical nanoparticles with a size of about 5 nm.

[0042] Preparation of NaHTe solution: take 79.75 mg Te powder, 56.7 mg NaBH 4 Add 5ml of deoxygenated ultrapure water into a flask filled with nitrogen, and heat it to about 70~90°C under constant stirring, and bubbles can be observed. The reaction was completed when no obvious bubbles emerged, and the pink solution obtained at this time was the NaHTe solution.

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Abstract

The invention relates to a quantum dot / polymer composite nitric oxide fluorescent probe and a preparation method thereof. The fluorescent probe is a powdered solid and is prepared from a quantum dot and methyl methacrylate (MMA) by an in-situ composite method, wherein the quantum dot and MMA polymer are combined though a covalent bond. The preparation method comprises the following steps of: 1) dispersing and dissolving water-soluble cadmium salt in a mixed solution of the MMA and a coupling agent, performing prepolymerization reaction at the temperature of between 70 and 90 DEG C under the action of an initiator under the protection of N2, and stopping reacting when the viscosity of a system is approximate to that of glycerol; 2) adding an aqueous solution of a sulfur source, a selenium source or a tellurium source into the prepolymer system in the step 1) dropwise with violent stirring under the protection of the N2 until the system is quickly discolored, wherein the molar ratio of the sulfur source, the selenium source or the tellurium source to the cadmium salt is 1.1:1-1.4:1; and 3) polymerizing at the temperature of between 50 and 70 DEG C for 10 to 24 hours. The fluorescent probe has the double advantages of the quantum dot and the polymer, is high in stability and is suitable for detecting nitric oxide in a sample to be detected.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a nitric oxide fluorescent probe and a preparation method thereof. Background technique [0002] In the late 1980s, the discovery of the endogenous gas signal molecule nitric oxide (NO) created a new research field of gas signal molecules. NO is a small molecular gas with free radical properties, which is fugitive and unstable. Easy to be oxidized and other characteristics. It is produced endogenously in the body, exerts a wide range of biological effects, and has unique characteristics such as continuous production, rapid transmission, and rapid diffusion. A large number of studies have proved that gas signal molecules, as a unique vasoactive substance, participate in and mediate various physiological processes of the central nervous system, such as learning, memory, nerve signal transmission, etc., as well as pathophysiological processes of cerebral ischemia a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L33/12C08K3/30C08K3/28C08F220/14C09K11/56C09K11/88G01N21/64
Inventor 丁莉芸黄俊袁银权钟韵鸣李大鹏范超童伊琳
Owner GUANGDONG NANHAI ETETB TECH CO LTD
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