Supercharge Your Innovation With Domain-Expert AI Agents!

Method for extracting lycopodine from lycopodium clavatum

A technology of lycoporine and sarcocarpus, which is applied in the field of natural medicinal chemicals, can solve problems such as complex ingredients, and achieve the effects of simple operation, environmental protection, and less solvent

Inactive Publication Date: 2012-07-25
苏州宝泽堂医药科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are few existing methods for extracting lycoperme from Streptococcus chinensis, because the alkaloids in Streptococcus spp.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take Shenjin herb and crush it into 80-100 mesh, weigh 1kg, add 5L of 90% methanol for reflux extraction for 1 hour, filter the extract, add 3L of 90% methanol for reflux extraction for 1 hour, filter the extract and add 3L of reflux for extraction for 1 hour, combine Recover methanol from the extract to extract, add 120ml of 2% acetic acid aqueous solution to dissolve, filter, add 240g of calcium oxide to dry, add 500ml of petroleum ether for ultrasonic extraction for 1 hour, filter out petroleum ether, add 600ml of chloroform for ultrasonic extraction for 1 hour, and chloroform extraction Chloroform is recovered from the liquid, add 20g of silica gel (120-200) mesh to dry, and 200g of silica gel is packed into the column, chloroform methanol mixed solution (33:9) is eluted, thin-layer detection is collected high-concentration fraction recovery reagent, and then uses anhydrous Dissolved in ethanol and recrystallized three times, and dried to obtain 805 mg of pure lycopi...

Embodiment 2

[0019] Take Shenjin herb and crush it into 80-100 meshes, weigh 1kg, add 5L99% methanol for reflux extraction for 1 hour, filter the extract, add 5L99% methanol for reflux extraction for 1 hour, combine the extracts to recover methanol to the extract, add 100ml 4% Dissolve in acetic acid aqueous solution, filter, add 200g of calcium oxide to dry, add 400ml of petroleum ether for ultrasonic extraction for 1 hour, filter out the petroleum ether, add 500ml of chloroform for ultrasonic extraction for 1 hour, recover chloroform from the chloroform extract, add 30g of silica gel (120-200 ) order drying, and 150g of silica gel column packing, chloroform methanol mixed solution (27:9) eluting, thin-layer detection, collects high-concentration fraction recovery reagent, dissolves and recrystallizes 2 times with dehydrated alcohol again, dries to obtain lycoperme Pure product 826mg, content 95.5%.

Embodiment 3

[0021] Take Shenjin herb and crush it into 80-100 mesh, weigh 10kg, add 50L 90% methanol for reflux extraction for 1 hour, filter the extract, add 30L 90% methanol for reflux extraction for 1 hour, combine the extracts to recover methanol to the extract, add 1L 2% Dissolve acetic acid aqueous solution, filter, add 2.5kg of calcium oxide to dry, add 4L of petroleum ether for ultrasonic extraction for 1 hour, filter out the petroleum ether, add 6L of chloroform for ultrasonic extraction for 1 hour, recover chloroform from the chloroform extract, add 200g of silica gel (120- 200) mesh drying, and 1000g silica gel column packing, chloroform-methanol mixed solution (31:9) elution, TLC detection, collect high-concentration fractions to recover reagents, then dissolve and recrystallize 3 times with absolute ethanol, and dry to obtain Lycopodium The pure alkali product is 8.15g, the content is 97.1%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting lycopodine from lycopodium clavatum. The method comprises the following steps of: smashing the lycopodium clavatum to 80-100 meshes; adding 90-99% methanol the amount of which is 3-5 times that of the lycopodium clavatum, and carrying out reflux extraction for two-three times; recovering methanol from an extract solution; adding an acetic acid water solution in extract for dissolution; filtering, and then adding calcium oxide for drying; successively carrying out ultrasonic extraction with petroleum ether and chloroform; recovering chloroform from a chloroform extraction solution; adding silica gel for drying, and packing; and eluting a chloroform methanol mixed solution; carrying out a thin film test, collecting high-concentration flow fraction and recovering reagents; and carrying out dissolution and recrystallization for 2-3 times with absolute ethyl alcohol, and drying a crystal substance so as to obtain the pure lycopodine product. According to the invention, the method is adopted to produce the lycopodine, so as to reduce environmental pollution; and the method has the advantages of good stability and high specificity.

Description

Technical field: [0001] The invention belongs to the field of natural medicine and chemistry, and in particular relates to a method for extracting lycoperme from Herba chinensis. Background technique: [0002] Lycoperine is a quinolizidine alkaloid with molecular formula C 16 h 25 NO, molecular weight 247.38, columnar crystal, melting point 116°C. Optical rotation -24.5 (c=1.1, ethanol). Soluble in benzene and chloroform, etc. [0003] Lycoperpine is an alkaloid extracted from Lycopodium, which has antihypertensive, antipyretic, antibacterial and excitatory effects. [0004] Streptococcus japonicum is the rhizome and root of the dry rooted whole plant of Lycopodium japonicum Thunb. in Lycopodium japonicum Thunb. It dispels wind and cold, dehumidifies and reduces swelling, and has the functions of relaxing tendons and activating collaterals. [0005] Existing methods for extracting lycoperme from Streptococcus sinensis are few, because the alkaloids in Streptococcus sin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D455/03
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com