Method for increasing crystalizing efficiency of xylose in xylose mother solution

Abstract

The invention belongs to the fields of biology and chemical industry, and particularly discloses a method for increasing the crystalizing efficiency of xylose in a xylose mother solution. The method comprises the following steps of: diluting the xylose mother solution; adding nutritional components; sterilizing; inoculating yeast for fermenting; removing glucose and galactose from the xylose mother solution; centrifugally separating yeast out of a zymotic fluid; filtering a clear zymotic fluid with a cross flow tube film; and removing a colloid contained in the zymotic fluid and fermentation metabolic products such as free proteins and the like produced in a fermenting process to obtain an ultra-filtered clear solution containing xylose and arabinose for use in subsequent production. The method has the advantages of simple steps, low treatment cost, high separating accuracy, good separating effect, great reduction in the subsequent treatment difficulty, great increase in product yield and suitability for wide popularization and application.

Description

(1) Technical field [0001] The invention belongs to the fields of biology and chemical industry, and particularly relates to a method for improving the crystallization efficiency of xylose in xylose mother liquor. (2) Background technology [0002] Xylose products are mainly white crystals made from corn cobs and bagasse through hydrolysis, purification, evaporation, crystallization, centrifugation, drying and other processes. They are widely used in light industry, food and chemical industries. The concentration of xylose mother liquor is about 60%, of which xylose is 40~50%, in addition, glucose is 15~20%, arabinose is 15~30%, galactose is 8~12%, due to the existence of these components, xylose mother liquor cannot Crystallized and sold only as a by-product. [0003] Although it has been reported to use a simulated moving bed to purify the xylose mother liquor to produce xylose and arabinose (patent number 200710014175.8), and it has been produced on a large scale, th...

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Problems solved by technology

[0003] Although it has been reported to use a simulated moving bed to purify the xylose mother liquor to produce xylose and arabinose (patent number 200710014175.8), and it has been produced on a large scale, the pretreatment of the xylose mother liquor is not thorough, and the galactose, colloid, The metabolites produced by protein and yeast fermentation and the broken bacteria cannot be completely removed, which brings great difficulty to decolorization and filtration

Due to the traditional vacuum drum filtration and plate-and-frame filtration process, the direction of liquid flow is consistent with the direction of filtration. Impurities such as colloids in the liquid will directly adhere to the filter medium quickly, blocking the filter medium and unable to remove all impurities in the liquid. Colloid and protein and other substances contained in it, the fermentation broth after decolorization is generally filtered by plate and frame, and the average filtration capacity per square meter of filter cloth is 2~3m 3 , and then the filter cloth has to be replaced and cleaned; in addition, a large amount of filter aids such as diatomaceous earth are consumed in the vacuum drum filtration process, which increases the operating cost of filtration on the one hand, and the filter aids can be taken away Part of the material increases the loss of the material; finally, due to the very close distance between the HPLC peaks of xylose and galactose, the simulated moving bed separation fluid cannot remove galactose from the xylose component, resulting in the presence of most of the galactose In the xylose component, the purity of xylose is greatly reduced, and the presence of galactose makes the yield of xylose crystallization about 30%.