Wet composite dressing and sealed negative pressure drainage device therefrom
A technology of negative pressure drainage and wet dressing, which is applied in the field of biomedicine, can solve the problems of high cost, high cost of polyvinyl alcohol, and dependence on imports, and achieve high tensile strength and elastic modulus. The combination process is simple and easy, and the preparation The process is simple and easy to get the effect
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Embodiment 1
[0029] (1) Bacterial cellulose is prepared by Acetobacter xylinum, and the activated strain is placed in the seed medium to obtain a seed solution. The seed medium is 10w / v% glucose, 0.01w / v% calcium carbonate, 0.01w / v% yeast extract and the balance water. The cultured seed solution is inserted into the fermentation medium to obtain bacterial cellulose. The fermentation medium is 3w / v% glucose, 0.1 w / v% disodium hydrogen phosphate, 0.1 w / v% potassium dihydrogen phosphate, 0.1 w / v% yeast extract, 0.1 w / v% peptone and the rest water. Soak bacterial cellulose obtained by fermentation culture in 1% NaOH aqueous solution by mass, heat at 60°C for 6 hours, and then rinse repeatedly with double distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane.
[0030] (2) Dissolve chitosan with a molecular weight of 50,000 in 0.1% acetic acid solution to prepare a 5% chitosan acetic acid solution. The bacterial cellulose with a...
Embodiment 2
[0033] (1) Bacterial cellulose is prepared from Rhizobium, and the activated strain is placed in the seed medium to obtain a seed solution. The seed medium is 3w / v% glucose, 0.1w / v% calcium carbonate, 0.1w / v% yeast extract and the balance water. The cultured seed solution is inserted into the fermentation medium to obtain bacterial cellulose. The fermentation medium is 10w / v% glucose, 0.4 w / v% disodium hydrogen phosphate, 0.4 w / v% potassium dihydrogen phosphate, 0.5 w / v% yeast extract, 0.5 w / v% peptone and the rest water. Soak the bacterial cellulose obtained by fermentation culture in 2% NaOH aqueous solution by mass, heat at 70°C for 5 hours, and then wash it repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane.
[0034] (2) Dissolve chitosan with a molecular weight of 100,000 in 0.5% acetic acid solution to prepare a 4% chitosan acetic acid solution. The bacterial cellulose with a wat...
Embodiment 3
[0037] (1) Bacterial cellulose is prepared from Sarcina, and the activated strain is placed in the seed medium to obtain a seed solution. The seed medium is 5w / v% glucose, 0.05w / v% calcium carbonate, 0.02w / v% yeast extract and the rest of water. The cultured seed solution is inserted into the fermentation medium to obtain bacterial cellulose. The fermentation medium is 5w / v% glucose, 0.2w / v% disodium hydrogen phosphate, 0.2w / v% potassium dihydrogen phosphate, 0.2w / v% yeast extract, 0.2w / v% peptone and the rest of water. Soak bacterial cellulose obtained through fermentation culture in 3% NaOH aqueous solution by mass, heat at 80°C for 4 hours, and then rinse repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane.
[0038] (2) Dissolve chitosan with a molecular weight of 150,000 in 1% acetic acid solution to prepare a 3% chitosan acetic acid solution. The bacterial cellulose with a water ma...
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