Method for performing enzymolysis and enrichment on alpha-linolenic acid (ALA) with perilla frutescens oil

Abstract

The invention provides a method for performing enzymolysis and enrichment on alpha-linolenic acid (ALA) with perilla frutescens oil, relates to biotechnologies and fine chemical engineering technologies and mainly aims at reaching a key control point in the enrichment of the high-purity ALA with the same adjacent carbon atoms and the different ethylenic bonds so as to improve the content of the ALA in the perilla frutescens oil to more than or equal to 90% from 62.4% through enrichment. The method comprises the following specific steps of: performing enzymolysis on the perilla frutescens oil by lipase to obtain mixed fatty acid, and separating the mixed fatty acid to saturated fatty acid and mixed unsaturated fatty acid through a urea envelope method so as to obtain a solid phase and a liquid phase; extracting the saturated fatty acid from the solid phase with the recovery rate reaching 61%; extracting oleic acid, linoleic acid and ALA from the liquid phase, and performing temperature-gradient cooling on the liquid phase to obtain a solid phase and a liquid phase; extracting oleic acid and linoleic acid from the solid phase with the recovery rate reaching 59%; and extracting ALA from the liquid phase with the recovery rate reaching 51% and the content being 93.47%. The method has the most outstanding characteristic of enrichment of the high-purity ALA from the plant oil, thus having particularly remarkable social and economic benefits.

Description

technical field [0001] The invention relates to biological enzyme technology and oil fine chemical complexation and freezing technology, and belongs to a method for enzymatic hydrolysis and enrichment of α-linolenic acid of perilla seed oil combined with biological technology and fine chemical industry. Background technique [0002] Due to the adverse effects of world industrialization on the social ecological environment and human health, governments and people of various countries have paid more and more attention. Separating and purifying biologically active substances from natural resources to regulate the physiological functions of the human body and prevent various chronic diseases has become a food product. Research hotspots in science, medicine, and nutrition. Such substances not only have the nutritional function of general food, but also have the function of regulating physiological functions, and have been widely used in the field of functional health products. A...

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Problems solved by technology

[0004] In our country, there is a kind of plant resources rich in α-linolenic acid, and their research has attracted great attention from many domestic research institutes, but we are the same as some developed countries in terms of applying the latest high-tech separation and purification to obtain high-purity α-linolenic acid Compared with us, there is still a certain gap. We can only sell low-purity products as raw materials or intermediates. The added value of products is low, and resource consumption is serious, while high-purity products often need to be imported.