Method for analyzing active component in harmel grass

A technology of camel grass and active ingredients, which is applied in the analysis of materials, material separation, measuring devices, etc., can solve the problems that cannot fully reflect the quality level of camel grass, achieve excellent reproducibility and specificity, good stability, Simple operation effect

Active Publication Date: 2013-12-11
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the standard does not carry out exclusive qualitative and quantitative analysis of the active ingredients, and cannot fully reflect the quality level of Camelina chinensis, which has become a bottleneck in the research and development of the medicinal value of Camelina chinensis.

Method used

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  • Method for analyzing active component in harmel grass
  • Method for analyzing active component in harmel grass
  • Method for analyzing active component in harmel grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: Construction of TLC fingerprint

[0051] a) Preparation of the test solution

[0052] Take 1g of camel grass powder, add 10mL of methanol, ultrasonically treat at 160~250W, 40kHz frequency for 30min, cool, filter with 0.45μm nylon microporous membrane, evaporate the filtrate, add 2mL of methanol to dissolve the residue, take the supernatant, That is the test solution.

[0053] b) Preparation of reference substance solution

[0054] Take 1 mg of each of dehydrohaeline, harmine and orchidine reference substances, and prepare solutions with 1 mL of methanol respectively, which is the reference substance solution;

[0055] c) Take 5 μL each of the above-mentioned test solution and reference solution, and spot them on fluorescent silica gel high-efficiency thin-layer plates respectively, with a mixed solvent formed by ethyl acetate-methanol-ammonia in a volume ratio of 10:1.5:0.5. Developing agent, developed, taken out, after drying, inspected under 254nm ul...

Embodiment 2

[0061] Embodiment 2: construct HPLC fingerprint atlas

[0062] a) Preparation of the test solution

[0063] Take 0.5g of camel grass powder, add 10mL of 70% methanol aqueous solution by volume, ultrasonically treat it at 160~250W, 40kHz frequency for 30 minutes, take it out, place it at room temperature, and make up the lost weight with 70% methanol, Shake well, and filter with 0.45 μm nylon microporous membrane, which is the test solution;

[0064] b) Preparation of reference substance solution

[0065] Accurately weigh the appropriate amount of orchidine, salicine, dehydrohaeline, deacetylated salicin and salicin, accurately weighed, and add methanol to prepare 80 μg of orthine and salicine per 1 mL The solution of 20 μg, 20 μg of dehydrohaevalline, 50 μg of deacetylated camelvalin and 50 μg of camelvalin is the reference solution;

[0066] c) Precisely draw 10 μL each of the test solution and the reference solution, and inject them into the high-performance liquid chroma...

Embodiment 3

[0081] Embodiment 3: to the assay of active ingredient

[0082] a) Preparation of the test solution

[0083] Take 0.5g of camel grass powder, add 10mL of 70% methanol aqueous solution by volume, ultrasonically treat it at 160~250W, 40kHz frequency for 30 minutes, take it out, place it at room temperature, and make up the lost weight with 70% methanol, Shake well, and filter with 0.45 μm nylon microporous membrane, which is the test solution;

[0084] b) Precisely draw 10 μL of the test solution, inject it into a high-performance liquid chromatograph for determination, and the high-performance liquid chromatographic conditions are as follows: the chromatographic column is a Boston Symmetrix ODS-AQ column (4.6mm×250mm), the mobile phase A is acetonitrile, and the mobile phase B is a 0.1% aqueous trifluoroacetic acid solution by volume, and the elution gradient is as follows:

[0085]From 0 to 5 minutes, the volume of mobile phase A is 5%, and the volume of mobile phase B is 95...

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Abstract

The invention discloses a method for analyzing an active component in harmel grass. The method comprises the following steps of: constructing a finger print, analyzing the active component in a qualitative manner through the finger print, detecting the content of the active component through an HPLC (high performance liquid chromatography) method, wherein the finger print comprises a TLC (thin-layer chromatography) finger print and / or HPLC finger print. According to the invention, the constructed finger print has excellent reproducibility and specificity, and has good stability, precision and application sample recovery ratio on the content detection method of the active component, can realize qualitative and quantitative analysis of various active components such as vasicine, peganine, harmine, deacetylation harmel glycoside and harmel glycoside in the harmel grass, can fully evaluate the quality of the harmel grass, and can provide scientific reference for medicinal value of research and development on harmel grass; and the analyzing method provided by the invention has the advantages of simplicity in operation, velocity, economy, strong operability and the like, and can carry out popularization and application.

Description

technical field [0001] The invention relates to a method for analyzing effective components in camel grass, belonging to the technical field of traditional Chinese medicine analysis. Background technique [0002] Camel pomegranate is a medicinal material that has been used by Uyghur and Mongolian people for a long time, and has been included in the Uyghur medicine sub-volume of the People's Republic of China's Health Products and Drug Standards. Camel florets are flat in nature, bitter, pungent, and poisonous. It has the functions of strengthening tendons, helping yang and warming yin, eliminating viscous body fluids, and dissipating cold and damp air. Indications for weakness of tendons, arthralgia, cough with profuse phlegm, hemiplegia, forgetfulness, dizziness, headache, and irregular menstruation. In Xinjiang, Gansu, Qinghai, Ningxia, Inner Mongolia and Shaanxi in the northwestern part of my country, the desert, sandy, dry grassland and other areas are mainly distribute...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/90
Inventor 王长虹程雪梅王峥涛温方方
Owner SHANGHAI UNIV OF T C M
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