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Fluorescent material and preparation method and application thereof

A fluorescent molecular imprinting and raw material technology, applied in the field of fluorescent materials and molecular imprinting, can solve the problems of complex operation, poor specificity, and inability to detect quickly.

Inactive Publication Date: 2012-08-22
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The task of the present invention is to provide a fluorescent material and a fluorescent molecularly imprinted polymer synthesized using the fluorescent material as a fluorescent functional monomer, so that the synthesized fluorescent molecularly imprinted polymer has a simple and reliable preparation process, stable chemical activity, and recyclable , It can realize the rapid detection and quantitative analysis of environmental endocrine disruptors under the conditions of aqueous solution, room temperature, near neutrality and without introducing any special external energy (referring to ultrasound, microwave heating, strong light irradiation, etc.), so as to overcome The existing technology is complex in the detection of environmental endocrine disruptors, has poor specificity, and cannot be quickly detected.

Method used

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  • Fluorescent material and preparation method and application thereof
  • Fluorescent material and preparation method and application thereof
  • Fluorescent material and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: Synthesis and characterization of dansylmethacrylate

[0036] Mix 0.1mol / L dansyl chloride with 0.1mol / L aminoethylmethacrylamide hydrochloride aqueous solution, add diethylamine as a catalyst, stir at 5°C for 12h, evaporate the resulting solution to dryness, and use chloroform:water The 1:1 extract was extracted 5 times, and the water in the organic phase was removed with anhydrous sodium sulfate to obtain bright yellow dansylmethacrylic acid solution.

[0037]The resulting bright yellow dansylmethacrylate solution was evaporated to dryness to an oily substance, and the oily substance was separated through a silica gel column, and impurities were removed using an eluent of petroleum ether:ethyl acetate at a ratio of 1:1, and the obtained product had a purity of 95 More than dansylmethacrylate fluorescent material.

[0038] The dansylmethacrylate fluorescent material synthesized in Example 1 was used for the following characterizations:

[0039] (1) Diss...

Embodiment 2

[0041] Example 2: Fluorescent MIPs synthesized using dansylmethacrylate prepared by the present invention as a functional monomer combined with BPA are used to indirectly measure the content of BPA

[0042] Dissolve 5 mg / L MIP in 5 mL of acetonitrile, vortex for 1 minute, and sonicate for 30 minutes to obtain a solution with a concentration of 1 g / L. Take 1 mg of the solution from the above mother liquor, dissolve it in 9 mL of acetonitrile, vortex for 1 minute, and sonicate for 30 minutes. Minutes, get 0.1g / L solution, dilute in the same way to get 2mg / L solution and then add different concentrations of BPA (BPA concentrations are 0, 5, 15, 25, 35, 45 mg / L). At this time, the fluorescent MIPs will combine with BPA to undergo fluorescence quenching, and the maximum emission wavelength of the fluorescence is 515nm. After the reaction, the fluorescence intensity of the fluorescent MIPs acetonitrile solution at 515 nm was measured on a fluorescence spectrophotometer. from Imag...

Embodiment 3

[0043] Example 3: Fluorescent MIPs synthesized using dansylmethacrylate prepared by the present invention as a functional monomer combined with BPZ, BPC, E2, and BPA are used to determine the specificity of fluorescent MIPs binding to BPA

[0044] Dissolve 5 mg / L MIP in 5 mL of acetonitrile, vortex for 1 minute, and sonicate for 30 minutes to obtain a solution with a concentration of 1 g / L. Take 1 mg of the solution from the above mother liquor, dissolve it in 9 mL of acetonitrile, vortex for 1 minute, and sonicate for 30 minutes. Minutes, 0.1g / L solution was obtained, and diluted in the same way to obtain 2mg / L solution. Then add BPC+BPZ+E2 and different concentrations of BPA (A: Control; B: BPC+BPZ+E2 (2.5mg / Leach); C: B+5mg / L; D: B+15mg / L; E: B+25mg / L; F: B+35mg / L), at this time, fluorescent MIPs will combine with BPA to undergo fluorescence quenching, and the maximum emission wavelength of the fluorescence is 515nm. After the reaction, measure the fluorescence intensity o...

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Abstract

The invention provides a fluorescent material, and also provides a fluorescent molecularly imprinted polymer prepared by the fluorescent material. The fluorescent material is dansyl methacrylic acid, and the preparation method comprises the following steps: performing a sulfonylation reaction of dansyl chloride and aminoethyl methacrylamide hydrochloric acid under the action of a catalyst to obtain a dansyl methacrylic acid solution, performing extraction and purification of the obtained solution to obtain the dansyl chloride fluorescent material of dansyl methacrylic acid. A molecularly imprinted polymer synthesized by using the dansyl methacrylic acid fluorescent material of the invention as a functional monomer not only can be used as a traditional molecular recognition material for specific separation and combination of target template molecules, but also can be used as a sensing material for direct rapid qualitative and quantitative analysis of endocrine disruptors in environment and food.

Description

technical field [0001] The invention belongs to the technical field of fluorescent materials and molecular imprinting, and specifically relates to a fluorescent material that can be used for synthesizing fluorescent molecularly imprinted polymers (fluorescent MIPs) and a preparation method thereof, and fluorescent molecular imprinting prepared using the fluorescent material as a functional monomer polymer. technical background [0002] Environmental endocrine disruptors refer to a large class of substances that can interfere with the normal endocrine function of humans or animals, causing sex changes or developmental deformities of humans or animals. Such compounds are stable in structure and exist in trace concentrations in the environment. Other constant substances in the sample can easily interfere with the accurate quantification of such trace pollutants. Therefore, how to accurately and quickly detect the content of trace environmental endocrine disruptors has always be...

Claims

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Application Information

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IPC IPC(8): C07C311/40C07C303/38C09K11/06G01N21/64C08F220/38C08J9/26B01J20/26
Inventor 吕斌伍雅婷叶磊
Owner HUAZHONG UNIV OF SCI & TECH
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