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Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts

A technology of Bacillus subtilis and Bacillus subtilis is applied in the field of fermented ginseng and natto, and can solve the problems of immaturity, low production yield, difficult separation and purification of ginsenosides and their transformed products, and the like

Inactive Publication Date: 2012-09-12
金光洙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current research situation is concerned, the method of producing highly active ginsenosides by microbial transformation is not very mature, and there are many difficult problems to solve
For example, complex transformation products, low production yield, difficult separation and purification of ginsenoside and its transformation products, etc.

Method used

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  • Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts
  • Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts
  • Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Fermented ginseng preparation. Take ginseng raw materials: any one or more of fresh ginseng, dried ginseng, red ginseng, white ginseng, tail ginseng, and ginseng leaves; Add 5-20% weight of ginseng raw material to Bacillus subtilis bacterial liquid, Bacillus subtilis is preferably Bacillus subtils IFO 3007, Bacillus natto Sawamura 06 or Bacillus vatto Sawamura IFO 3339, cultured at 25-45°C and relative humidity 40-95% 24-72 hours ， The biotransformation fermented ginseng is obtained, and the biotransformation fermented ginseng product contains at least one of the following components: ginsenoside Rg 2 , Rg 3 、Rh 1 、Rh 2 , Ginsenoside G-F 1 , CK, F 2 .

Embodiment 2

[0028] Cut 100g of ginseng into slices, crush them, pass through a 40-80 mesh sieve, moisten with water with 20%-100% weight of ginseng raw materials, spray into 5-20% weight of ginseng raw materials with Bacillus subtilis bacteria liquid, cultivate for 48 hours, and dry the culture , reflux extraction with 75%-95% ethanol aqueous solution twice for 2-3 hours each time, recover ethanol to obtain ethanol extract, ethanol extract is passed through D101 macroporous adsorption resin, and eluted with 75% ethanol to obtain total saponins of converted human energy.

Embodiment 3

[0030] Finely chop 100g of fresh ginseng, mash it and sterilize it, spray it into the Bacillus subtilis bacterial liquid, cultivate it for 48 hours, dry the culture, extract it with 75% ethanol, recover the ethanol to obtain the ethanol extract, and the ethanol extract is absorbed through D101 macropores The resin was eluted with 75% ethanol to obtain the total saponins of converted human energy.

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Abstract

The invention discloses fermented ginseng obtained through fermentation of bacillus subtilis. Ginseng raw materials are crushed and are moistened by water, bacillus subtilis liquid is sprayed, the culture is carried out for 24 to 72 hours to obtain biotransformation fermented ginseng; the ginseng raw materials are one kind or several kinds of materials from fresh ginseng, dry ginseng, red ginseng, white ginseng, tail ginseng and ginseng leaves; the bacillus subtilis can metabolize ginsenoside ingredients to generate any one kind of bacillus subtilis of biotransformation bodies, the characteristic rare ginseng contents are improved through the obtained biotransformation fermented ginseng, and the content proportion is respectively more than 2 to 10 times higher than that before the fermentation. The invention also discloses fermented ginseng natto obtained after the fermentation of ginseng and soybeans through the bacillus subtilis. The invention also discloses an application of extracts obtained through extracting the fermented ginseng fermented by bacillus subtilis to the preparation of fermented beverages, fermented ginseng particles or fermented ginseng capsule preparations.

Description

technical field [0001] The invention relates to a Bacillus subtilis fermentation product of ginseng prepared by fermenting ginseng with Bacillus subtilis, a fermented ginseng natto prepared by simultaneously fermenting ginseng and soybean with Bacillus subtilis, and a Bacillus subtilis fermentation product of ginseng The application of the extract obtained from the extraction is carried out. Background technique [0002] Ginseng is the dried root of Panax ginseng (Panaxginsongc.A.Meyer), an herbaceous plant of the Araliaceae family. The main component of ginseng is ginsenoside. More than 50 kinds of ginsenoside monomer compounds have been isolated from Panax genus plants, which have anti-tumor, Anti-fatigue, anti-aging, anti-shock, anti-virus and anti-inflammation, anti-infection, anti-tissue damage, anti-neurocyte apoptosis, anti-stress substance metabolism, nootropic, treatment of senile dementia, improvement of heart function, lowering blood sugar, strengthening the body ...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/20A23L2/38A23L33/00A23L11/50
Inventor 金光洙潘元丽李英姬
Owner 金光洙