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Biocontrol strain PA-2 (Pseudomonas Aeruginosa) for preventing and curing tobacco angular leaf spot

A technology for tobacco angular spot disease and biocontrol strains, which is applied in the directions of bacteria, biocides, microorganisms, etc., can solve problems such as varieties that have not been cultivated to resist angular leaf spot disease, and adverse effects on the safety of cigarettes exported from tobacco leaves. The effect of pollution production and cost reduction

Inactive Publication Date: 2014-08-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is quite difficult to prevent the occurrence of tobacco angular leaf spot by using agricultural techniques such as crop rotation; due to the long-term use of chemical agents in large quantities, pathogenic bacteria have developed resistance, and at the same time have adverse effects on tobacco leaf export and cigarette safety; Varieties resistant to angular leaf spot

Method used

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  • Biocontrol strain PA-2 (Pseudomonas Aeruginosa) for preventing and curing tobacco angular leaf spot
  • Biocontrol strain PA-2 (Pseudomonas Aeruginosa) for preventing and curing tobacco angular leaf spot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 can control the biocontrol bacteria-Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) Screening of PA-2

[0020] Specific steps are as follows:

[0021] Isolation of bacteria from barnyardgrass: cut about 2 g from the barnyardgrass plant with sterilized scissors, put it in a sterilized mortar, add 1 mL of sterile water to grind it into a homogenate, and serially dilute the homogenate 10 times to 10 - 7 times, will be 10 - 4 to 10 - 7 100 μL of each dilution was drawn and spread on the NA plate, each concentration was repeated three times, and cultured at 28°C for 2-3 days. Pick different types of colonies, streak and purify them on the NA plate, pick a single colony and transfer it to a PDA slant for numbering and storage for later use; it can also be numbered and stored in 40% glycerol and stored in a -80°C refrigerator.

[0022] Preparation of bacterial fermentation broth: pick out the bacterial cultures of Xanthomonas tabacum and isolated from barny...

Embodiment 2

[0035] Example 2 Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) Optimization of fermentation conditions for PA-2 (Accession No. CGMCC No. 5937)

[0036] The bacterial strain is Pseudomonas aeruginosa (Pseudomonas aeruginosa) PA-2 (preservation number CGMCC No. 5937)

[0037] Specific steps are as follows:

[0038] Single factor experiment: NB culture medium was used as the basal medium, and different single carbon sources were selected (glucose, sucrose, lactose, xylose, mannitol maltose, soluble starch and glycerol, with an amount of 0.5%); Select different single organic nitrogen sources (tryptone, peptone, beef extract and yeast extract, dosage 1%), different single inorganic nitrogen sources (NH4 Cl, (NH 4 ) 2 SO 4 、KNO 3 and NH 4 NO 3 , dosage 1%); in the case of better carbon source and nitrogen source, add different concentrations of NaCl (0.1%, 0.3% and 0.5%); better carbon source, nitrogen source and NaCl, add different concentrations of inorganic nitrogen ...

Embodiment 3

[0042] Example 3 Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) Field efficacy test of PA-2 (Accession No. CGMCC No. 5937) on tobacco angular leaf spot.

[0043] Pseudomonas aeruginosa ( Pseudomonas aeruginosa )PA-2 (Accession No. CGMCC No. 5937)

[0044] There are 15 tobacco plants in each plot, and the plant spacing is 0.9 m×0.5 m. A total of 5 treatments were set up: A biocontrol bacteria PA-2 viable fermentation broth (6-fold dilution); B biocontrol bacteria PA-2 optimized fermentation broth (6-fold dilution); C NB culture medium control (6-fold dilution solution); D optimized NB culture medium control (6-fold dilution); E pharmaceutical streptomycin (3000-fold dilution). Arranged in random blocks and repeated 3 times. Each treatment was sprayed for the first time by high-pressure spraying method at the tree cluster stage, and A, B, C, D, and E were sprayed with each treatment liquid, and each treatment was sprayed with 2 L. Two days later, each treatment was spray...

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Abstract

The invention belongs to the technical field of plant protection, which discloses a biocontrol strain PA-2 (Pseudomonas Aeruginosa) for preventing and curing the tobacco angular leaf spot. The strain is collected in the China General Microbiological Culture Collection Center on 27th March, 2012 with a collection number of CGMCC (China General Microbiological Culture Collection Center) No.5937. The total concentration of biocontrol bacterial liquid prepared from the biocontrol strain PA-2 is 1*10<9>-1*10<10>CFU (Colony Forming Units) / mL. The biocontrol bacterial liquid can be used for preventing and curing the tobacco angular leaf spot and the biocontrol strain is a strain with a great research and development value.

Description

technical field [0001] The present invention relates to a strain of biocontrol bacterial strain PA-2, and this bacterial strain is Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), which can prevent and treat tobacco angular leaf spot, and belongs to the technical field of plant protection. Background technique [0002] Tobacco angular leaf spot is caused by Pseudomonas syringae pv. tobacco [ Pseudomonas syingae pv. tabaci (Wolf et Foster.)] infection is one of the most serious and devastating tobacco bacterial diseases. Tobacco angular leaf spot has spread all over the main tobacco-producing areas in Asia, Africa, Europe, and North and South America. When the environmental conditions are conducive to the occurrence of the disease, it can cause tobacco production to die out. [0003] From 2003 to 2007, the annual incidence area of ​​tobacco angular leaf spot in my country was about 33,300 hm 2 , resulting in an annual output value loss of 26.43 to 57.82 million yua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/385
Inventor 文景芝孙剑萍杨晓敏所冰田苗
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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