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Phenyl modified material for inhibiting cancer cell proliferation

A technology for modifying materials and phenyl groups, applied in the field of phenyl-modified materials, can solve the problems of unsatisfactory treatment effect, postoperative complications, recurrence, and more metastasis.

Inactive Publication Date: 2012-10-17
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the clinically discovered middle-advanced patients, the overall treatment effect is not satisfactory, and there are many postoperative complications, recurrence, and metastasis

Method used

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  • Phenyl modified material for inhibiting cancer cell proliferation
  • Phenyl modified material for inhibiting cancer cell proliferation
  • Phenyl modified material for inhibiting cancer cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, preparation phenyl modified material

[0028] 1) With phenyl-containing mercaptan [molecular formula is C 6 h 6 (CH 2 ) 8 SH] as the solute, absolute ethanol as the solvent, seal and shake well, prepare a thiol solution with a mass concentration of 1.0%, and keep away from light throughout. Stand at 4°C for 24h to make the thiol molecules evenly distributed in the solution.

[0029] 2) Clean the monocrystalline silicon (Si,100) sheet twice in a mixture of concentrated sulfuric acid (mass concentration 98%) and hydrogen peroxide (mass concentration 50%) with a volume ratio of 3:1, and then wash it with triple distilled water for 3 times. Next, dry and seal them under a nitrogen environment. Put the cleaned single crystal silicon wafer into the vacuum chamber of the plasma sputtering equipment, turn on the titanium source, grow a titanium (Ti) transition layer (thickness is 10nm); finally turn off the titanium source, turn on the gold source, on the tit...

Embodiment 2

[0033] Embodiment 2, the in vitro cell test of the effect of phenyl modified material and cancer cells

[0034] 1) Put the 1.0cm×1.0cm phenyl-modified material (prepared in Example 1) into a 24-well plate after sterilization, add breast cancer cells, 10 4 cells / well and placed in a 37°C incubator until the cells adhered to the wall.

[0035] 2) Qualitative observation: Electron microscope detection to record the adhesion and proliferation of breast cancer cells.

[0036] 3) Quantitative detection: Real-time quantitative PCR to detect the expression of estrogen receptor 2 gene (ESR2), migration and proliferation-related genes (Snaill, Cyclln) in breast cancer cells.

[0037] Research object: human breast cancer epithelial cells (MCF-7). This cancer cell line was positive for the Wnt7h gene.

[0038] The result is as follows:

[0039] 1. Adhesion result

[0040] The gold flakes grafted with phenyl chemical functional groups were cut into slices with a side length of 1.0 cm,...

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Abstract

The invention discloses a phenyl modified material, a preparation method and an application thereof. The phenyl modified material is prepared according to the following steps: 1) adopting mercaptan containing phenyl as a solute and adopting anhydrous ethanol as a solvent to prepare a mercaptan solution with a mass concentration of 0.8-1.5%, and standing the solution for 12-24 hours at a temperature of 3-8 DEG C, wherein the molecular formula of the mercaptan containing phenyl is C6H6(CH2)nSH, and n is a positive integer; 2) growing a titanium (Ti) transition layer on a surface of a single crystal silicon wafer, and growing a gold layer on the Ti transition layer to obtain a gold-plated silicon wafer; 3) under a dark condition, adding the gold-plated silicon wafer from the step 2) to the prepared mercaptan solution from the step 1), and standing for 12-24 hours to obtain the phenyl modified material. The phenyl modified material of the present invention can inhibit adhesion, migration and proliferation of breast cancer cells.

Description

technical field [0001] The invention relates to a phenyl-modified material for inhibiting proliferation of cancer cells. Background technique [0002] Breast cancer is the most common malignant tumor in women, accounting for 30% of new malignant tumors in women. In recent years, the incidence of breast cancer has remained high, and the treatment methods include surgery, radiotherapy, chemotherapy, endocrine therapy and molecular targeted therapy. At present, the curative effect of breast cancer depends more on the stage of the disease, and people place more hope on the prevention and improvement of early diagnosis rate in the treatment of breast cancer. For the clinically discovered middle-advanced patients, the overall treatment effect is not satisfactory, and there are many postoperative complications, recurrence, and metastasis. Therefore, it is still necessary to find more new ideas and methods for the prevention and treatment of breast cancer, as well as the preventio...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/16C12Q1/02
Inventor 于晓龙须苏菊崔福斋
Owner TSINGHUA UNIV
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