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Heterogeneous inverted flow chip and preparation method thereof

A flow chip and heterogeneous technology, applied in the field of micro-system chips, can solve the problems of insufficient safety and stability of detection probes, affecting chip quality and life, and poor packaging, etc., to achieve mass production, low cost, Effect of material and processing cost reduction

Active Publication Date: 2012-10-17
BEIJING BIOSYNTHESIS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages of difficult packaging
In addition, no matter on the chip used in the laboratory or commercially, the detection probes are arranged on the upper surface of the support body, and the marking surface of the detection probes and the detection probes are exposed outside or are not tightly packaged, which are easy to be damaged. pollution, and the detection probe is inevitably affected by external variables such as pressure, the safety and stability of the detection probe are relatively insufficient, which affects the quality and life of the chip

Method used

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  • Heterogeneous inverted flow chip and preparation method thereof
  • Heterogeneous inverted flow chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of the lower support:

[0026] The lower support is made of PMMA sheet. Do the quality inspection of the board before production. The board should be of uniform thickness, smooth surface, no scratches on the surface and with a protective film, and the thickness of the board is 30mm. Cut the slabs into blanks of the designed shape with a stone cutting machine.

[0027] Use a fixed fixture to fix the blank, and use a milling machine to cut and repair the excess part. The cutting amount is 2mm, the feed rate is 250mm / min, the speed is 1000 rpm, the drilling speed is 1440 rpm, and the sinking drilling speed is 1440 rpm. The processing temperature is 20°C.

[0028] The blank processed by the milling machine is laser etched, and the micro-guiding groove of the flow chip is processed according to the given trajectory. The laser intensity is 50W, the moving speed is 250mm / s, and the temperature of the laser beam irradiation point is controlled at 370°C.

[0029]...

Embodiment 2

[0041] The preparation of the upper and lower supports is the same as in Example 1.

[0042] Chip preparation: After cleaning the chip substrate, air-dry the chip with dust-free low-humidity air at a temperature of 50°C at a flow rate of 0.1m / s for 30-45 seconds. After the chip substrate is dried, carry out antibody detection probes according to the antibody labeling process mark. Apply the fixing glue on the chip base, and dry it in dust-free low-humidity air at a temperature of 50°C at a flow rate of 0.1m / s for 15-20 seconds. The fixing glue on the chip base is in a semi-dry state.

[0043] The preparation of the monolithic chip is the same as in Example 1.

[0044] This embodiment can be used for protein molecular detection. Taking lactate dehydrogenase as an example, this test uses GENMED SCIENTICS INC U.S.A’s reagent as an example. Take 100ul of Tris-lactic acid buffer solution labeled as reaction solution A and mix it with 20ul of the reaction sample, react at 25°C for...

Embodiment 3

[0046] The preparation of the upper and lower supports is the same as in Example 1.

[0047] Chip preparation: After the chip substrate is cleaned and dried, the chip is air-dried in dust-free low-humidity air at a temperature of 50°C at a flow rate of 0.1m / s for 30-45 seconds. After the chip substrate is dried, nucleic acid detection is carried out according to the antibody labeling process Probe labeling. Apply the fixing glue on the chip base, and dry it in dust-free low-humidity air at a temperature of 50°C at a flow rate of 0.1m / s for 15-20 seconds. The fixing glue on the chip base is in a semi-dry state.

[0048] The preparation of the monolithic chip is the same as in Example 1.

[0049] This embodiment can be used for protein detection. Taking early pregnancy detection as an example, collect about 100ul of morning urine and inject it from the sampling port at one end of the upper support, and inject 100ul of the reaction solution from the other sampling port at the s...

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Abstract

The invention discloses a heterogeneous inverted flow chip and a preparation method thereof. The heterogeneous inverted flow chip includes an upper supporter and a lower supporter made of a polymethylmethacrylate material and a glass chip base encapsulated between the upper and the lower supporter. The upper supporter is provided with a slot corresponding to the chip base; the lower supporter is provided with a micro diversion trench with a port extending out of the chip edge; a plurality of detection probes are arranged on a chip side close to the lower supporter; the arrangement position of the detection probes is corresponding to the micro diversion trench;and the upper supporter is provided with a conical micro diversion hole corresponding to the micro diversion trench. The present invention provides a reliable, low-cost and novel flow chip convenient for mass production; the method fully uses characteristics of mature processing technology and low cost of the PMMA material; a set microchannel provides reaction space for the experiment; and the detection probes are arranged in a downwards way on glass sheets convenient for fixing markers. The heterogeneous inverted flow chip has advantages of novel and reasonable structure, and greatly reduced material and processing cost.

Description

technical field [0001] The invention relates to a microsystem chip, in particular to a heterogeneous inverted flow chip and a preparation method thereof. Background technique [0002] The flow chip comes from the early miniaturized total analysis system (Miniaturized Total Analysis System, μ-TAS). The miniaturized total analysis system refers to the integration of multiple common operations in the laboratory into a chip of a few square centimeters through miniaturization and automation technology. A technique used to complete different biological or chemical reaction processes and analyze their products. Flow chip technology is one of the rapidly developing frontier fields of high-tech and interdisciplinary science and technology. It is an important technical platform for the research of signal detection and processing methods in life sciences, chemical sciences and information sciences. The flow chip has the characteristics of high integration, fast analysis speed, large d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 白向阳
Owner BEIJING BIOSYNTHESIS BIOTECH