Process for jointly detecting estrogens and their associations in livestock and poultry excrements

Abstract

The invention relates to a technology for detecting endocrine disrupters in the environment, and especially relates to a technology for quantitatively analyzing estrone (E1), 17beta-estradiol (E2), estriol (E3), 17alpha-ethinyl estradiol (EE2), estrone-3-glucuronide (E1-3G), 17beta-estradiol-3-glucuronide (E2-3G), estrone-3-sulfate (E1-3S) and 17beta-estradiol-3-sulfate (E2-3S) in a complex matrix livestock and poultry excrement sample through adopting a liquid chromatography-tandem mass spectrometry technique. The technology comprises the following steps: extracting estrogens in the collected livestock and poultry excrement sample through adopting a rapid solvent extraction method, pre-purifying the resulting extract through liquid-liquid extraction and alkaline solution extraction, enriching targets with an HLB column, purifying estrogen monomers and estrogen associations through using a Florisil column and an NH2 column respectively, and finally detecting through adopting the liquid chromatography-tandem mass spectrometry technique. The process has the advantages of environmental pollution, easy operation, and high recovery rate, and allows the estrogens and their associations having trace amounts in the complex matrix livestock and poultry excrements to be rapidly analyzed.

Description

technical field [0001] The invention relates to the technical field of liquid chromatography-tandem mass spectrometry detection, in particular to the extraction, purification and instrument detection methods of estrogen and its conjugates in complex livestock and poultry feces samples. Background technique [0002] In recent years, natural and synthetic estrogens estrone (Estrone, E1), 17β-estradiol (17β-Estradiol, E2), estriol (Estriol, E3) and 17α-ethinyl estradiol (17α-Ethinyl Estradiol , EE2) as a typical environmental endocrine disruptor has aroused widespread concern in the academic community. Relevant investigations and studies have shown that the ng / L concentration level in the water environment can pose a feminization risk to aquatic organisms such as fish. The urine and feces discharged by humans and mammals are the most fundamental sources of estrogen in the environment, and their discharge is inevitable. Estrogen is metabolized by the liver, and is mainly excre...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to develop a high recovery rate, high sensitivity, accurate and fast analysis method for the problems of trace amounts of estrogen and conjugates in livestock and poultry feces samples with complex matrix, large matrix interference, and difficult pretreatment technology. technology to achieve quantitative determination of estrogen and its conjugates, such as E1, E2, E3, EE2, E1-3S, E2-3S, E1-3G, E2-3G

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