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Medium for simple and quick separation of phytophthora melonis

A culture medium and Phytophthora technology, applied in the field of microbiology, can solve the problems of low isolation rate of target bacteria, difficult isolation, and failure to achieve separation and purification, and achieve the effects of fast speed, simple preparation, practicality, quickness and cost

Active Publication Date: 2012-10-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual work, in addition to being infected by Phytophthora, the samples collected from the field inevitably often contain other non-target fungi and bacteria such as Fusarium, Rhizoctonia solani and Pythium, and isolate the pathogenic bacteria from the tissue according to the conventional method However, due to the contamination of these non-target fungi or bacteria, the isolation rate of the target bacteria is low or it is difficult to isolate the target bacteria
Therefore, when using ordinary medium to isolate Phytophthora from diseased tissue or soil, the purpose of separation and purification cannot be achieved.

Method used

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  • Medium for simple and quick separation of phytophthora melonis
  • Medium for simple and quick separation of phytophthora melonis
  • Medium for simple and quick separation of phytophthora melonis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The inhibitory effect of embodiment 1, four kinds of fungal inhibitors and three kinds of antibiotics to Phytophthora meloni

[0032] 1. Inhibitory effect of four fungal inhibitors on Phytophthora meloni

[0033] Using acetone or methanol as the solvent of prochloraz, fludioxonil, hymexazol and pentachloronitrobenzene, prepare 10ml of four medicament mother solutions of 5000 μg / mL respectively; wherein, prochloraz, fludioxonil and pentachloronitrobenzene The mother liquors of the three fungal inhibitors of nitrobenzene were respectively prepared with a serial concentration of 50 μg / mL, 20 μg / mL and 10 μg / mL drug-containing PDA plates; 100μg / mL, 70μg / mL and 50μg / mL drug-containing PDA plates, and the plate with solvent as a control; then the mycelial growth rate method was used to determine the effect of Phytophthora meloni on prochloraz, fludioxonil, and hymexazol and pentachloronitrobenzene sensitivity. Pre-cultivate the Phytophthora melonii strain to be tested on a ...

Embodiment 2

[0042] Embodiment 2, three kinds of antibiotics are to the inhibitory effect of bacteria in the environment

[0043] Plants to be tested: the susceptible cucumber variety Changchun Mici was cultivated in a seedling pot with a diameter of 15 cm and a height of 12 cm, and placed in the pepper test field of the Science Park of China Agricultural University for cultivation. After the plants grew to the 4-5 leaf stage, the rhizomes were collected immediately for Preparation of bacterial suspension.

[0044] Soil for testing: Soil samples were collected from areas where the melon disease occurred seriously in Xiqing District, Tianjin in 2009, for future use.

[0045] Test method: This test is based on the results of Example 1 to detect whether the selected three concentrations of antibiotics can effectively inhibit the bacteria in the environment. For the detection of bacteria in the environment, it is mainly aimed at the bacteria from the base of the plant stem and the diseased so...

Embodiment 3

[0048] Embodiment 3, be used to isolate the PDA selective culture medium of Phytophthora cucurbitus

[0049] 1. Separation method

[0050] In 2009 and 2011, disease samples were collected from Xiqing District and Hexi District, Tianjin, where melon blight was severe, for the isolation of Phytophthora meloni.

[0051] The isolation of Phytophthora meloni is carried out according to the following method: different fields are selected in the same area, and disease samples (rhizome and soil) of the disease are collected for isolation.

[0052] Separation step: clean the diseased tissue with clean water and dry it fully, cut out a small piece of tissue at the junction of the rhizome with disease and health, inoculate it on the selective medium of the present invention, and culture it in the dark at 25°C until mycelium grows; Take the fungus cake from the edge of the colony, put it in a petri dish with 10mL of sterilized water, and culture it under the light condition of 28°C for 2...

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Abstract

The invention discloses a medium for simple and quick separation of phytophthora melonis. The medium is prepared by adding first bactericide or second bactericide in a PDA (potato dextrose agar) medium, wherein the first bactericide is composed of fludioxonil, hymexazol, quintozene and rifampicin, and the second bactericide is composed of prochloraz, hymexazol, quintozene and rifampicin. Experiments show that the PDA selective medium is practicable, accurate, practical, quick, low in cost and simple to prepare. Separation of phytophthora oomycetes such as phytophthora melonis by the PDA selective medium can solve the problem that non-target fungi and bacteria are badly polluted in the process of phytophthora separation, and is fast, efficient, timesaving and laborsaving.

Description

technical field [0001] The invention relates to a simple and fast culture medium for isolating Phytophthora meloni. It belongs to the technical field of microorganisms. Background technique [0002] Plant diseases caused by Phytophthora are highly destructive and harmful to host plants, spread and spread rapidly, and most of the diseases caused are difficult to control. In one growing season of plants, the pathogens can develop rapidly and cause disease epidemics, resulting in serious agricultural production. loss. Plant diseases caused by Phytophthora are therefore commonly referred to as "blight". For example, potato late blight first became popular in North America in 1835, and then continued to occur in Europe in 1845. In 1847, due to the outbreak of the disease in Ireland, potato production was almost extinct, leading to the famous famine in history. Millions of people starved to death, and more than 2 million emigrated. Soybean blight caused by Phytophthora sojae i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/18A01N47/38A01N43/90A01N43/84A01N33/18A01N43/36A01N43/80A01P3/00A01P1/00C12N1/14C12R1/645
Inventor 贾睿哲刘鹏飞
Owner CHINA AGRI UNIV
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