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Microbial foliage fertilizer, and preparation method and application thereof

A microbial and foliar fertilizer technology, applied in application, fertilization device, fertilizer mixture, etc., can solve the problems of yield, quality, disease resistance and pest resistance that cannot meet the demand, few types of bacteria, and insufficient nutrition.

Active Publication Date: 2014-08-27
山东劲土生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The foliar fertilizer described in the above patent has a certain promoting effect on the growth of plants, but the nutrition is not comprehensive enough, and the types of bacteria are few, and the effect of improving crop yield, quality, disease resistance and pest resistance cannot meet the demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The preparation method of above-mentioned microbial foliage fertilizer, comprises the steps:

[0022] (1) Inoculate Bacillus subtilis ACCC03120, Bacillus jelly-like CICC23640, Bacillus megaterium CICC21694, and Bacillus thuringiensis CICC20565 respectively in liquid seed medium under aseptic conditions, and culture them at 30-37°C for 18-24 hours, Then inoculate in the liquid fermentation medium according to the inoculum amount of 10-20wt%, and cultivate at 30-37 DEG C for 3-5 days;

[0023] The number of live bacteria containing Bacillus subtilis ACCC03120 was 3~8×10 respectively. 8 Each / mL of bacterial agent A, containing jelly-like bacillus CICC23640, the number of viable bacteria is 3~8×10 8 Bacteria B per mL, containing Bacillus megaterium CICC21694, the number of live bacteria is 1~5×10 8 Each / mL of bacterial agent C, containing Bacillus thuringiensis CICC20565, the number of viable bacteria is 3~8×10 8 Individual / mL of bacterial agent D;

[0024] (2) Inoculat...

Embodiment 1

[0060] A preparation method of microbial foliage fertilizer, comprising the steps of:

[0061] (1) Inoculate Bacillus subtilis ACCC03120, Bacillus jelly-like CICC23640, Bacillus megaterium CICC21694, and Bacillus thuringiensis CICC20565 respectively in liquid seed medium under aseptic conditions, cultivate them at 30°C for 24 hours, and then press 10wt% The inoculum amount was inoculated in the liquid fermentation medium and cultivated at 30°C for 3 days;

[0062] The number of live bacteria containing Bacillus subtilis ACCC03120 was 8×10 respectively. 8 Bacteria agent A of each / mL, containing jelly-like Bacillus CICC23640, the number of viable bacteria is 6×10 8 Bacteria agent B containing Bacillus megaterium CICC21694 count 4×10 per mL 8 Each / mL of bacterial agent C, containing Bacillus thuringiensis CICC20565 viable count 6×10 8 Individual / mL of bacterial agent D;

[0063] The seed culture medium in the described step (1) is prepared by mixing and sterilizing the follow...

Embodiment 2

[0086] The microbial foliar fertilizer obtained by the preparation method as described in Example 1, the difference is that in step (4), the prepared bacterial agent A, bacterial agent B, bacterial agent C, bacterial agent D, bacterial agent E and bacterial agent F are mixed according to the volume ratio of 1:1:2:2:2:2 to prepare a composite microbial bacterial agent with a total bacterial count of 5×10 8 individual / mL.

[0087] Among them: the number of live bacteria of Bacillus subtilis (Bacillus subtilis) ACCC03120 8×10 7 Individual / mL, Bacillus mucilaginosus (Bacillus mucilaginosus) CICC23640 viable count 6×10 7 Individual / mL, Bacillus megaterium (Bacillus megaterium) CICC21694 viable count 8×10 7 Individual / mL, Bacillus thuringiensis (Bacillus thuringiensis) CICC20565 live bacteria count 12×10 7 Individual / mL, Azotobacter chroococcum (Azotobacter chroococcum) ACCC11104 live bacteria count 10×10 7 Individual / mL, Saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC1...

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Abstract

The invention relates to a microbial foliage fertilizer, and a preparation method and application thereof. Every liter of microbial foliage fertilizer is prepared by mixing the following raw materials: 0.3-0.5L of microbial zymogen liquid, 0.2-0.3L of trace element solution, 0.05-0.15kg of K2SO4, 0.05-0.15kg of NH3H2PO4, 0.1-0.25kg of urea, 0.05-0.15kg of yeast fermentation powder slag, 3-4g of diethylaminoethanol hexanoate and the balance of water. Multiple microbes with the functions of nitrogen fixation, phosphorus decomposition, potassium decomposition and disinsection are added into the foliage fertilizer, thereby being beneficial to enhancing the crop yield, quality and disease / insect resistance.

Description

technical field [0001] The invention relates to a microbial foliar fertilizer and its preparation method and application, belonging to the technical field of microbial fertilizers. Background technique [0002] There are two main ways for plants to absorb nutrients: roots and leaves. Among them, the method of applying fertilizer to the surface of the vegetative body other than the root system of the crop is called root fertilization, also known as foliar fertilization. Fertilizers used for foliar application are called foliar fertilizers. [0003] Since the foliar fertilizer is directly sprayed on the leaves without passing through the soil, it has the following characteristics: (1) High fertilizer efficiency. The foliar fertilizer is directly sprayed on the leaves, and the utilization rate is high, which can reduce the total amount of fertilizer. (2) Fast nutrient absorption and good fertilizer efficiency. There is no need for long transportation processes such as root ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00
Inventor 展彬李环环杨瑞
Owner 山东劲土生态农业科技有限公司