Method for extracting and separating L-valine from fermentation liquor by employing membrane separation and electroosmosis combined technology

Abstract

The invention discloses a method for extracting and separating L-valine from fermentation liquor by employing membrane separation and electroosmosis combined technology and belongs to the technical field of biochemical engineering. The method comprises the steps of (1) removing thallus from fermentation liquor; (2) carrying out electroosmosis to clear fermentation liquor to remove inorganic salt; (3) removing small-molecule protein and pigment impurities form clear desalted liquid; (4) carrying out preconcentration to the fermentation liquor with reverse osmosis membrance, and (5) concentrating and crystallizing the fermentation liquor. The method disclosed by the invention has the characteristics that the condition is mild, the operation is simple, the separation steps are less and the selectivity is good; the defects of low yield, large sewage discharge and high production intensity of the existing ion exchange technology are overcome, and the yield and quality of the L-valine are significantly improved.

Description

Technical field [0001] The invention relates to a method for extracting and separating L-valine, in particular to a method for separating and extracting inorganic salts from L-valine fermentation broth using a combination of membrane separation and electrodialysis technology, and belongs to the technical field of biochemical industry . Background technique [0002] As one of the branched chain amino acids, L-valine is an essential amino acid for the human body and has a variety of physiological functions. Because of its special structure and function, it occupies a particularly important position in human life metabolism. It is mainly used to formulate compound amino acid infusion, synthetic peptide drugs and food antioxidants, etc., especially its role in medical research and treatment, which has attracted increasing attention . It is used in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic...

AI Technical Summary

Problems solved by technology

The general process of this process is: filter the fermentation broth to remove bacteria, and ion exchange to separate amino acids through the process of adsorption and elution. Using this process to separate and extract L-valine from the fermentation broth has the following problems: one is to use filtration to remove bacteria Because the bacteria are small, usually the bacteria are not completely removed; the second is that the color value of the L-valine fermentation liquid is relatively high, and the decolorization of ordinary activated carbon is not complete and the dosage is large, which affects the product quality and yield; the third is L-valine There are many impurities and high content in the acid fermentation liquid, and the direct ion exchange method will cause serious resin pollution, which will easily cause the resin structure to be broken and lose its regeneration ability, which will increase the operating cost. At the same time, the high content of ionic substances will quickly saturate the resin , increase the number of resin regeneration, resulting in a large amount of sewage discharge and other problems

Membrane separation technology has been used in the separation and purification of fermentation products. There have been successful precedents, but the use of membrane separation and electrodialysis combined technology to separate and extract L-valine from fermentation broth has not been reported.

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