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A kind of aerosolized live vaccine of swine mycoplasma pneumonia and its preparation and testing method

A technology for Mycoplasma swine pneumonia and Mycoplasma pneumoniae, which is applied in material testing products, testing pharmaceutical preparations, bacterial antigen components, etc., can solve problems such as the reduction of the vitality of aerosol vaccines, and achieve the effects of promoting immunity, improving convenience, and reducing damage.

Active Publication Date: 2014-10-22
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to cutting force, high pressure and other forces, the factors that affect the activity of aerosol microorganisms include environmental factors, such as rapid evaporation of water in aerosolized vaccines in low-humidity and high-temperature environments, salt metabolism disorders, and rapid drying and oxidation. The effect greatly reduces the viability of aerosolized vaccines

Method used

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  • A kind of aerosolized live vaccine of swine mycoplasma pneumonia and its preparation and testing method
  • A kind of aerosolized live vaccine of swine mycoplasma pneumonia and its preparation and testing method
  • A kind of aerosolized live vaccine of swine mycoplasma pneumonia and its preparation and testing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Get 1 bottle of freeze-dried bacterial classification (2ml outfit / bottle) of Mycoplasma hyopneumoniae attenuated strain (168 strains), dissolve into the live mycoplasma hyopneumoniae with 2ml of deionized aqueous solution (pH value 6.8) containing 0.1% polyvinylpyrrolidone (PVP). Vaccine solution, the vaccine solution is added in the medical lower respiratory tract deposition type sprayer (Germany Bairui), the sprayer is connected to the air compressor pump, and the pressure is adjusted to 0.2MPa before spraying, and the live vaccine of Mycoplasma suis pneumonia is prepared as aerosolized live vaccine.

[0031] Connect the nozzle of the sprayer to a closed aerosol cabinet, and connect the sample inlet of the TSI3321 aerosol particle size analyzer to the aerosol cabinet through a pipeline. After 5 minutes of aerosolized vaccine, turn on the power supply of the TSI3321 aerosol particle size analyzer, and measure the aerodynamic median diameter of the total particles of the...

Embodiment 2

[0035] Get 10 bottles of freeze-dried strains of mycoplasma hyopneumoniae attenuated strain (168 strains) (2ml pack / bottle), dissolve into mycoplasma hyopneumoniae with 2000ml deionized aqueous solution (pH value 7.2) containing 0.5‰ propylene glycol block polyether (F68) Live vaccine solution, the vaccine solution is added to an electric sprayer (1035BP, Husson, USA) for spraying, and the flow rate is set to 300g / min to prepare an aerosolized live vaccine for Mycoplasma suis pneumonia.

[0036] Connect the nozzle of the sprayer to a closed aerosol cabinet, and connect the sample inlet of the TSI3321 aerosol particle size analyzer to the aerosol cabinet through a pipeline. 5 seconds after the aerosolized vaccine occurs, turn on the power of the TSI3321 aerosol particle size analyzer to detect the aerodynamic diameter of the total particles of the aerosol vaccine. Then connect the self-made atomized vaccine quantitative collection device to the aerosol cabinet to collect the va...

Embodiment 3

[0040]Get 200ml of freshly cultivated bacterium solution of Mycoplasma hyopneumoniae attenuated strain (168 strains), dilute it with 20000ml dehydrated ionized solution (pH value 7.2) containing 8% glycerin to become Mycoplasma hyopneumoniae live vaccine solution, and this vaccine solution is added to intermittent In the water tank of the self-pressurized high-pressure spray system (patent authorization announcement number: CN202238430U, applicant: Jiangsu Academy of Agricultural Sciences), the hydraulic pump is turned on for spraying, and the pressure is set to 3MPa to prepare a live aerosolized vaccine for Mycoplasma suis pneumonia.

[0041] The particle size of the aerosol vaccine was detected by soft cushion method. The specific method is: Grind 2 parts of paraffin oil and 1 part of Vaseline evenly, apply it on a glass slide with grooves, heat to remove the air bubbles, and then it is as image 3 Upholstered as shown. Place the cushion 30cm below the nozzle of the high-pr...

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Abstract

The invention discloses a swine mycoplasmal pneumonia atomized live vaccine which comprises vaccine strains and vaccine diluent. The vaccine strains are freeze-dried strains with mycoplasma hyopneumoniae attenuated strains (168 strains) or freshly prepared bacterial liquid. The vaccine diluent is deionized water solution with the pH (potential of hydrogen) value of 6.8-7.5 and containing 5-10% of glycerol in final concentration, or is deionized water solution containing 0.01-0.1% of propylene glycol block polyether (F68), or is deionized water solution containing 0.1% of polyvinyl pyrrolidone (PVP), or is an optional combination of protective agents. Besides, the invention further discloses a preparation and inspection method of the vaccine. The swine mycoplasmal pneumonia live vaccine is atomized, convenience in vaccination can be improved, labor power and labor time are greatly saved, the vaccine can be more effectively popularized for immunity, and the vaccine is applicable to the technical field of medicinal preparations.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and relates to a vaccine and its preparation and testing methods, in particular to a swine mycoplasma pneumonia atomized live vaccine and its preparation and testing methods. Background technique [0002] Mycoplasma pneumonia is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae infection. The disease spreads all over the world, and it is easy to have mixed infection with other pathogens, causing more serious lung lesions and greater economic losses. Vaccination is the main way to prevent the disease. At present, the vaccines available on the market are mainly divided into imported inactivated vaccines and domestic attenuated vaccines. After immunization with inactivated Mycoplasma pneumoniae vaccine, it cannot prevent the colonization of bronchial cilia by Mycoplasma hyopneumoniae wild virus, so it cannot produce complete protection. Mycopl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61P31/04G01N15/02G01N33/15
Inventor 冯志新邵国青刘茂军熊祺琰华利忠甘源
Owner JIANGSU ACAD OF AGRI SCI
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